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The map label for this gene is galS [H]

Identifier: 218690300

GI number: 218690300

Start: 2524359

End: 2525399

Strand: Reverse

Name: galS [H]

Synonym: ECED1_2598

Alternate gene names: 218690300

Gene position: 2525399-2524359 (Counterclockwise)

Preceding gene: 218690302

Following gene: 218690299

Centisome position: 48.48

GC content: 51.78

Gene sequence:

>1041_bases
ATGATCACCATTCGTGATGTAGCGCGTCAGGCTGGCGTCTCTGTGGCAACGGTTTCCCGGGTGCTCAATAACAGCACGCT
GGTCAGTGCCGATACGCGTGAAGCAGTAATGAAAGCCGTGAGTGAGCTGGATTATCGGCCAAACGCCAATGCCCAGGCGC
TGGCAACTCAGGTTAGCGATACCATTGGCGTGGTGGTGATGGACGTTTCTGATGCGTTTTTCGGCGCGCTGGTAAAAGCG
GTGGATCTGGTCGCCCAGCAGCATCAGAAATACGTGCTAATCGGCAATAGCTATCATGAAGCGGAAAAAGAGCGTCACGC
CATTGAGGTGTTAATTCGCCAGCGTTGTAATGCGTTGATTGTTCACTCAAAAGCATTGAGTGACGATGAACTGGCACAAT
TTATGGATAACATTCCCGGTATGGTGTTAATCAACCGCGTTGTGCCGGGGTACGCCCATCGTTGCGTTTGCCTGGATAAT
CTCAGCGGTGCCCGAATGGCGACGCGCATGTTGCTGAATAACGGTCATCAACGTATTGGTTATCTTTCTTCCAGCCACGG
CATTGAAGATGACGCCATGCGTAAAGCAGGCTGGATGAGTGCGTTGAAAGAGCAGGATATTATTCCGCCGGAAAGCTGGA
TTGGCACTGGCACGCCGGACATGCCGGGCGGTGAGGCGGCGATGGTTGAACTGCTGGGGCGCAATCTACAACTAACCGCT
GTATTTGCTTATAACGACAATATGGCCGCTGGCGCACTGACAGCATTAAAAGATAATGGCATTGCGATTCCGTTACATCT
CTCAATCATTGGTTTCGATGATATTCCCATCGCCCGTTACACCGACCCGCAATTAACGACTGTGCGTTATCCCATTGCTT
CAATGGCTAAATTAGCTACCGAACTGGCCTTGCAGGGGGCAGCAGACAATATTGATCCTCGTGCCAGCCACTGTTTTATG
CCGACGTTAGTGCGTCGCCATTCTGTCGCAACACGCCAGAATGCGGCGGCGATCACTAACTCAACAAATCAGGCGATGTA
A

Upstream 100 bases:

>100_bases
TCCATTGCTGTGACTCGATTCACGAAGTCCTGTATTCAGTGCTGACAAAATAGCCGCCAGCAAGCAGTCATTTACTGCAA
TCTCATAACAGGTAGTGAAT

Downstream 100 bases:

>100_bases
CCGCTTTCAATCTGTGAGTGATTTCACAGTATCTTAACAATGTGATGGCTATGATTGCACCGTTTTAACGTTGTAACCCG
TATGTAACAGTGAATAATCA

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM

Sequences:

>Translated_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM
>Mature_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=99.4219653179191, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=55.8912386706949, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=35.2238805970149, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=35.1791530944625, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=33.6336336336336, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=29.8360655737705, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=30.2547770700637, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=28.4375, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.8850574712644, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=25.7328990228013, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=25.9615384615385, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=26.6272189349112, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.7731958762887, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786268, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37445; Mature: 37445

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIG
EECCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEE
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAADNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIG
EECCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEE
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAADNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]