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The map label for this gene is dusC [H]

Identifier: 218690289

GI number: 218690289

Start: 2513166

End: 2514182

Strand: Reverse

Name: dusC [H]

Synonym: ECED1_2587

Alternate gene names: 218690289

Gene position: 2514182-2513166 (Counterclockwise)

Preceding gene: 218690297

Following gene: 218690286

Centisome position: 48.26

GC content: 53.0

Gene sequence:

>1017_bases
TTGCAGACCTTTAATCCGTTGACCGGAGGCGCTAACATAGCGCCTCATTTTTTTGCGGGTGATGATATGCGTGTGTTACT
GGCTCCGATGGAGGGTGTACTTGACTCTCTGGTGCGGGAATTACTGACCGAAGTTAACGACTACGATCTGTGCATCACCG
AGTTTGTCCGCGTGGTGGATCAACTGCTGCCAGTAAAAGTCTTTCATCGCATTTGCCCTGAGCTACAAAATGCCAGCCGG
ACACCATCCGGTACGCTGGTTCGCGTGCAGTTGTTAGGGCAGTTCCCGCAGTGGCTGGCAGAGAACGCCGCCCGTGCGGT
GGAGTTAGGTTCCTGGGGCGTGGATCTTAACTGCGGCTGCCCGTCGAAAACGGTTAACGGTAGCGGTGGTGGGGCAACGT
TACTGAAAGATCCTGAACTCATCTACCAGGGTGCAAAAGCGATGCGCGAAGCTGTACCGGCGCATTTGCCTGTCAGCGTA
AAAGTGCGTCTGGGCTGGGACAGCGGTGAGAAGAAATTTGAAATCGCCGATGCGGTTCAACAGGCTGGTGCTACGGAGCT
GGTAGTACATGGGCGGACAAAAGAGCAGGGTTACCGCGCGGAACATATTGACTGGCAGGCGATTGGCGAGATTCGCCAGC
GGCTTAATATTCCGGTGATTGCCAACGGTGAAATCTGGGACTGGCAGAGTGCGCAACAATGCATGGCGATCAGCGGCTGC
GACGCAGTGATGATTGGTCGCGGGGCGCTCAATATTCCCAACCTGAGCCGTGTGGTGAAATATAACGAACCGCGAATGCC
GTGGCCGGAGGTGGTTGCTTTGCTGCAAAAATATACCCGTCTGGAAAAGCAGGGCGATACCGGGTTATATCACGTTGCGC
GGATTAAACAGTGGTTGAGTTATTTGCGTAAAGAATACGATGAAGCAACGGAATTATTTCAGCATGTTCGGGTGTTGAAT
AATTCCCCTGATATTGCAAGGGCTATTCAGGCCATTGATATCGATAAGCTGCGATAA

Upstream 100 bases:

>100_bases
ATCAGCGCTTATTTTTTCGCTCCCCGGTATTACGCAATGCGCAGCGAACTCAGGCATACATCAAGCATAGAGAGGGATTG
CAGTTTGCAGCAATCCCTCT

Downstream 100 bases:

>100_bases
TAGTGGAATCGCAGCTTATCTGAGACAATGAATCGCTTATTCAGCTTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAG
CCACATATTGCCACTGGCGC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQTFNPLTGGANIAPHFFAGDDMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASR
TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSV
KVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLN
NSPDIARAIQAIDIDKLR

Sequences:

>Translated_338_residues
MQTFNPLTGGANIAPHFFAGDDMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASR
TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSV
KVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLN
NSPDIARAIQAIDIDKLR
>Mature_338_residues
MQTFNPLTGGANIAPHFFAGDDMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASR
TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSV
KVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLN
NSPDIARAIQAIDIDKLR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=28.6245353159851, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239788483, Length=227, Percent_Identity=32.5991189427313, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788462, Length=225, Percent_Identity=32, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=29.2887029288703, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=99.3650793650794, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=39.2857142857143, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.0649350649351, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=276, Percent_Identity=25.3623188405797, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=27.3972602739726, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585320, Length=217, Percent_Identity=30.8755760368664, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=29.608938547486, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=29.608938547486, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37660; Mature: 37660

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTFNPLTGGANIAPHFFAGDDMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVD
CCCCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGC
HHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCC
PSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQ
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
QAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCC
DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLS
CEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTFNPLTGGANIAPHFFAGDDMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVD
CCCCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGC
HHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCC
PSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQ
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
QAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCC
DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLS
CEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]