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The map label for this gene is thiM [H]

Identifier: 218690169

GI number: 218690169

Start: 2403731

End: 2404519

Strand: Reverse

Name: thiM [H]

Synonym: ECED1_2460

Alternate gene names: 218690169

Gene position: 2404519-2403731 (Counterclockwise)

Preceding gene: 218690170

Following gene: 218690168

Centisome position: 46.16

GC content: 54.5

Gene sequence:

>789_bases
ATGCAAGTCGACCTGCTGAGTTCAGCGCAATCTGCGCACGCGTTACACCTTTTTCACCAACATTCCCCTCTTGTGCACTG
CATGACCAACGATGTGGTGCAAACTTTTACTGCCAATACCTTGCTGGCGCTCGGCGCATCGCCAGCGATGGTTATCGAAA
CCGAAGAGGCCAGTCAGTTTGCGGCTATCGCCAGTGCCTTGTTGATTAACGTTGGCACGCTGACGCAGCCACGAGTGCAA
GCGATGAGTGCTGCCGTTGAGCAAGCAACACGATCACAAACGCCCTGGACGCTTGATCCGGTTGCGGTTGGTGCGCTCGA
TTATCGCCGCCGTTTTTGTGTGGAACTTCTGTCCCATAAGCCAACCGCCATACGTGGTAATGCTTCGGAAATCATGGCAT
TAGCTGGCGTTGCTAATGGTGGACGGGGAGTGGATACCACTGATGCCGCAGCTAACGCGATACCTGCTGCACAAACACTG
GCACGGGAAACTGGCGCAATCGTTGTGGTCACTGGCGAGGTGGATTATGTTACCGATGGACATCGCATTGTTGGTATTCA
CGGTGGCAATCCATTAATGACCAAAGTGGTAGGAACTGGCTGTGCACTATCGGCGGTTGTCGCTGCCTGTTGTGCGCTAC
CAGGCGATACGCTGGAAAATATCGCATCTGCCTGTCACTGGATGAAACAAGCCGGAGAACGCGCAGTCGCCAGAAGCGAG
GGGCCAGGTAGTTTTGTTCCACATTTCCTTGATGCGCTCTGGCAATTGGCGCAGGAGGTGCAGGCATGA

Upstream 100 bases:

>100_bases
GGCTGAGAAATACCCGTATCACCTGATCTGGATAATGCCAGCGTAGGGAAGTCACGGACCACCAGGTCATTGCTTCTTCA
CGTTATGGCAGGAGCAAACT

Downstream 100 bases:

>100_bases
AACGAATTAACGCTCTGACGATTGCCGGTACTGATCCGAGTGGTGGTGCGGGGATTCAGGCCGATCTTAAAACCTTCTCG
GCACTTGGCGCTTATGGTTG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAIASALLINVGTLTQPRVQ
AMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEVDYVTDGHRIVGIHGGNPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLAQEVQA

Sequences:

>Translated_262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAIASALLINVGTLTQPRVQ
AMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEVDYVTDGHRIVGIHGGNPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLAQEVQA
>Mature_262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAIASALLINVGTLTQPRVQ
AMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEVDYVTDGHRIVGIHGGNPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLAQEVQA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=262, Percent_Identity=94.2748091603053, Blast_Score=486, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325042, Length=269, Percent_Identity=32.7137546468402, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27294; Mature: 27294

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
CCCCCHHCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHH
AAIASALLINVGTLTQPRVQAMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
PTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEVDYVTDG
CCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEECCEEEEECC
HRIVGIHGGNPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
CEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPGSFVPHFLDALWQLAQEVQA
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
CCCCCHHCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHH
AAIASALLINVGTLTQPRVQAMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
PTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEVDYVTDG
CCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEECCEEEEECC
HRIVGIHGGNPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
CEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPGSFVPHFLDALWQLAQEVQA
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA