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The map label for this gene is erfK

Identifier: 218690048

GI number: 218690048

Start: 2269432

End: 2270364

Strand: Reverse

Name: erfK

Synonym: ECED1_2326

Alternate gene names: 218690048

Gene position: 2270364-2269432 (Counterclockwise)

Preceding gene: 218690049

Following gene: 218690047

Centisome position: 43.58

GC content: 49.73

Gene sequence:

>933_bases
ATGCGTCGTGTAAATATTCTTTGCTCATTTGCTCTGCTTTTTGCCAGCCATACTAGCCTGGCGGTAACTTATCCATTACC
TCCAGAGGGTAGCCGTTTAGTGGGGCAGTCGTTTACTGTAACTGTTCCTGATCACAATACCCAGCCGCTGGAGACTTTTG
CCGCACAATACGGGCAAGGGTTAAGTAACATGCTGGAAGCGAACCCGGGCGCTGATGTTTTTTTGCCGAAGTCTGGCTCG
CAACTCACCATTCCGCAGCAACTGATTTTGCCCGACACTGTTCGTAAAGGGATTGTTGTTAACGTCGCTGAGATGCGTCT
TTATTACTACCCACCAGACAGTAATACTGTGGAAGTCTTTCCTATTGGTATCGGCCAGGCTGGGCGAGAAACCCCGCGTA
ACTGGGTGACTACCGTTGAACGTAAACAAGAAGCTCCAACCTGGACGCCAACGCCGAACACTCGGCGCGAATATGCGAAA
CGAGGGGAGAGTTTGCCCGCATTTGTTCCTGCGGGCCCCGATAATCCCATGGGGCTGTACGCGATTTATATTGGCAGGTT
GTATGCCATCCATGGTACCAATGCCAATTTTGGTATTGGGCTCCGGGTAAGTCAGGGCTGTATTCGTCTGCGCAATGACG
ATATCAAATATCTGTTTGATAATGTTCCTGTTGGGACGCGTGTGCAAATTATCGACCAGCCAGTAAAATACACCACTGAA
CCAGATGGCTCGAACTGGCTGGAAGTTCATGAGCCGTTGTCGCGCAATCGTGCAGAATATGAGTCTGACCGAAAAGTGCC
ATTGCCGGTAACCCCATCTTTGCGGGCGTTTATCAACGGGCAAGAAGTTGATGTAAATCGCGCAAATGCTGCGTTGCAAC
GTCGATCGGGAATGCCTGTGCAAATTAGTTCTGGTTCAAGACAGATGTTTTAA

Upstream 100 bases:

>100_bases
CTACCGGGGAATACGACTTCTGATTTGAACAGTTAAAAACGAAAAGTCTGATAAAAGCTATACTTAACGGATAGCTTTCG
CGACATAGGAAAGGGACATG

Downstream 100 bases:

>100_bases
GAGCGTTGGTAATTAGAAAGCAAAAAGCCTGCTAGAAAGCAGGCTTTTTTGAATTTGGCTCCTCTGACTGGACTCGAACC
AGTGGCATACGGATTAACAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVPDHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGS
QLTIPQQLILPDTVRKGIVVNVAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYAK
RGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKYTTE
PDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF

Sequences:

>Translated_310_residues
MRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVPDHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGS
QLTIPQQLILPDTVRKGIVVNVAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYAK
RGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKYTTE
PDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF
>Mature_310_residues
MRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVPDHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGS
QLTIPQQLILPDTVRKGIVVNVAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYAK
RGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKYTTE
PDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family

Homologues:

Organism=Escherichia coli, GI1788299, Length=310, Percent_Identity=100, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787040, Length=296, Percent_Identity=64.1891891891892, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787356, Length=285, Percent_Identity=49.4736842105263, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1787968, Length=311, Percent_Identity=45.3376205787781, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ERFK_ECOLI (P39176)

Other databases:

- EMBL:   U33333
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64963
- RefSeq:   AP_002591.1
- RefSeq:   NP_416494.1
- ProteinModelPortal:   P39176
- SMR:   P39176
- DIP:   DIP-9522N
- IntAct:   P39176
- MINT:   MINT-1229758
- STRING:   P39176
- EnsemblBacteria:   EBESCT00000001745
- EnsemblBacteria:   EBESCT00000014631
- GeneID:   945273
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1968
- KEGG:   eco:b1990
- EchoBASE:   EB2546
- EcoGene:   EG12682
- eggNOG:   COG1376
- GeneTree:   EBGT00050000008974
- HOGENOM:   HBG295778
- OMA:   SVEVLPI
- ProtClustDB:   PRK10190
- BioCyc:   EcoCyc:G7073-MONOMER
- Genevestigator:   P39176
- InterPro:   IPR005490

Pfam domain/function: PF03734 YkuD

EC number: NA

Molecular weight: Translated: 34412; Mature: 34412

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVPDHNTQPLETFAAQYGQG
CCEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHCCC
LSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVNVAEMRLYYYPPDSNTVEVF
HHHHHHCCCCCCEEEECCCCEEECCHHHCCCHHHHCCEEEEEEEEEEEEECCCCCEEEEE
PIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYAKRGESLPAFVPAGPDNPMGLY
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCEEE
AIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKYTTE
EEEEEEEEEEECCCCCEEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEEECCCEECCC
PDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNRANAALQRRSGMPV
CCCCCCEEECCCHHCCCHHHHCCCCCCCCCCCCHHHEECCCEEECHHHHHHHHHCCCCCE
QISSGSRQMF
EECCCCCCCC
>Mature Secondary Structure
MRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVPDHNTQPLETFAAQYGQG
CCEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHCCC
LSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVNVAEMRLYYYPPDSNTVEVF
HHHHHHCCCCCCEEEECCCCEEECCHHHCCCHHHHCCEEEEEEEEEEEEECCCCCEEEEE
PIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYAKRGESLPAFVPAGPDNPMGLY
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCEEE
AIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKYTTE
EEEEEEEEEEECCCCCEEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEEECCCEECCC
PDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNRANAALQRRSGMPV
CCCCCCEEECCCHHCCCHHHHCCCCCCCCCCCCHHHEECCCEEECHHHHHHHHHCCCCCE
QISSGSRQMF
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592411; 9097040; 9278503; 9298646