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The map label for this gene is nac

Identifier: 218690047

GI number: 218690047

Start: 2268057

End: 2268974

Strand: Reverse

Name: nac

Synonym: ECED1_2325

Alternate gene names: 218690047

Gene position: 2268974-2268057 (Counterclockwise)

Preceding gene: 218690048

Following gene: 218690046

Centisome position: 43.55

GC content: 48.15

Gene sequence:

>918_bases
ATGAACTTCAGACGCCTGAAATACTTCGTAAAAATTGTAGATATTGGTAGCCTGACCCAGGCTGCTGAAGTATTGCATAT
CGCACAACCAGCGCTCAGCCAGCAGGTTGCCACACTGGAAGGTGAGTTAAATCAACAACTTTTGATCCGTACAAAGCGGG
GCGTTACACCAACAGACGCCGGAAAAATTCTCTATACCCATGCGCGGGCCATTTTACGTCAGTGTGAACAGGCCCAACTG
GCGGTGCATAACGTTGGTCAGGCATTATCGGGGCAAGTCTCGATTGGCTTTGCACCAGGAACCGCTGCGTCATCCATCAC
CATGCCCTTATTACAGGCGGTTCGCGCTGAATTTCCGGAGATCGTTATCTATCTTCATGAAAATAGTGGTGCAGTGCTTA
ACGAAAAATTGATAAATCACCAACTCGATATGGCGGTGATTTATGAGCATTCCCCTGTGGCTGGTGTATCCAGTCAGGCT
TTGCTGAAAGAAGATCTTTTTCTGGTAGGAACTCAAGATTGCCCGGGGCAAAGCGTTGATGTGAATGCTATTGCGCAAAT
GAACCTCTTTCTCCCCAGTGATTACAGTGCTATTAGACTTCGTGTTGATGAGGCTTTTTCCCTACGGCGACTCACGGCAA
AAGTTATTGGTGAAATTGAGTCTATTGCCACGCTTACCGCAGCGATTGCCAGCGGCATGGGCGTTGCAGTATTACCCGAA
TCGGCCGCGCGTTCGTTATGTGGCGCAGTAAATGGGTGGATGTCACGCATTACCACTCCTTCCATGAGTCTCTCTTTGTC
ATTAAATTTACCCGCCAGAGCGAACTTATCGCCACAGGCACAGGCAGTGAAAGAGTTGTTAATGTCAGTGATTAGTTCTC
CAGTGATGGAAAAAAGGCAGTGGCAATTGGTGAGCTAA

Upstream 100 bases:

>100_bases
TAGCTTGTACATCAACACCAAAATAAAACTGGCAAGCATCTTGCAATCTGGTTGTAAGTAATGGCGGCACTTGGGCCGAT
TCTTAAAAACCGGAGGCAAC

Downstream 100 bases:

>100_bases
GTGTTATATCGGGATGGAATAAGATGCGGGTTTTTATTATTTGTTATGCCGGGCATTAGACTTTAACAATAACGGGAAAT
CTGAACTGCCCGGAGTTTAC

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Sequences:

>Translated_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS
>Mature_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=100, Blast_Score=619, Evalue=1e-179,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=28.2786885245902, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=29.6747967479675, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=33.1428571428571, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=257, Percent_Identity=26.0700389105058, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=23.109243697479, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=278, Percent_Identity=24.4604316546763, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786202, Length=248, Percent_Identity=24.5967741935484, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=258, Percent_Identity=24.4186046511628, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=250, Percent_Identity=26.8, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAC_ECOLI (Q47005)

Other databases:

- EMBL:   U56736
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   L31639
- PIR:   D64963
- RefSeq:   AP_002590.1
- RefSeq:   NP_416493.1
- ProteinModelPortal:   Q47005
- SMR:   Q47005
- IntAct:   Q47005
- STRING:   Q47005
- EnsemblBacteria:   EBESCT00000000273
- EnsemblBacteria:   EBESCT00000015611
- GeneID:   946501
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1967
- KEGG:   eco:b1988
- EchoBASE:   EB4013
- EcoGene:   EG14265
- eggNOG:   COG0583
- GeneTree:   EBGT00070000031692
- HOGENOM:   HBG685425
- OMA:   HAQVILR
- ProtClustDB:   PRK11233
- BioCyc:   EcoCyc:G7072-MONOMER
- Genevestigator:   Q47005
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32836; Mature: 32836

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHHEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHHEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872