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The map label for this gene is rusA [H]

Identifier: 218689484

GI number: 218689484

Start: 1705422

End: 1705781

Strand: Reverse

Name: rusA [H]

Synonym: ECED1_1729

Alternate gene names: 218689484

Gene position: 1705781-1705422 (Counterclockwise)

Preceding gene: 218689485

Following gene: 218689483

Centisome position: 32.74

GC content: 52.5

Gene sequence:

>360_bases
ATGCTCATAGATTTGGTTTTACCTTACCCGCCGACGGTGAACACTTACTGGCGACGCCGTGGCAGCACATATTTTGTATC
AAAAGCCGGGGAGCGTTATCGCCGGGCAGTGTCGCTTATTGTTCGCCAGCAGCGACTGAAATTAAGCCTGTCCGGACGGC
TGGCAATAAAAATTATTGCAGAGCCACCGGATAAGCGTCGTCGTGACCTGGACAATATTCTGAAAGCACCGCTGGATGCG
CTGACGCACGCGGGGTTGTTAATGGACGATGAGCAGTTTGATGAAATCAATATTGTGCGTGGCCTGCCTGTTCCTGGCGG
GCGGCTGGGGATAAAAATCACAGAACTGGAGTGCGCATGA

Upstream 100 bases:

>100_bases
GCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACTGGCGTGC
TTGGGTAAAAGAGGTGACTG

Downstream 100 bases:

>100_bases
ATAACCAGTATTTACAGTTTGTTCGTGAGCAACTCATGATTGCCACTGCGGATCTCAGTGGGGCGACAAAAGGCCAGCTG
GAAGCCTGGCAGGAGAATGC

Product: putative crossover junction endodeoxyribonuclease RusA

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVSLIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGLPVPGGRLGIKITELECA

Sequences:

>Translated_119_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVSLIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGLPVPGGRLGIKITELECA
>Mature_119_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVSLIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGLPVPGGRLGIKITELECA

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=112, Percent_Identity=41.0714285714286, Blast_Score=90, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 13524; Mature: 13524

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVSLIVRQQRLKLSLSGRLAIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGLPVPGGRLGIKITELECA
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVSLIVRQQRLKLSLSGRLAIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGLPVPGGRLGIKITELECA
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA