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The map label for this gene is potI

Identifier: 218688639

GI number: 218688639

Start: 854082

End: 854927

Strand: Direct

Name: potI

Synonym: ECED1_0822

Alternate gene names: 218688639

Gene position: 854082-854927 (Clockwise)

Preceding gene: 218688638

Following gene: 218688640

Centisome position: 16.39

GC content: 54.96

Gene sequence:

>846_bases
ATGAATAATTTACCGGTGGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGTTGGGCTTCACCTTTCTCTACGCGCCAAT
GCTGATGCTTGTTATCTATTCGTTTAACAGCTCGAAGCTAGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGCG
AATTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCG
ATCCTCGGGACTATTGCGGCGGTCGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGTTTTGCCTTCATGATCAC
CGCACCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTGTTATTCGTCGCGCTTGCTCATGCTATTGGCT
GGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTCTGTACGGCTTATGTGGCAGTCGTTATTTCG
TCGCGTCTGCGGGAACTGGATCGCTCGATAGAAGAAGCCGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTTGT
CATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGA
TCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACTTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCGCGCGC
AGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGGCCTCAGCGGTAGCGATCATCATGTTGCTGCTGCTAATTATGCCGATAATGTGGTTCCACAAACATCAGCAA
AAAAGCGTGGGAGAACACGG

Downstream 100 bases:

>100_bases
AGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGA
TTTTTTAAGAAAACATCTTC

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG

Sequences:

>Translated_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG
>Mature_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=100, Blast_Score=552, Evalue=1e-159,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=40.5857740585774, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=31.25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=27.3584905660377, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POTI_ECOL6 (P0AFL2)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752920.1
- ProteinModelPortal:   P0AFL2
- SMR:   P0AFL2
- EnsemblBacteria:   EBESCT00000042990
- GeneID:   1040158
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0990
- GeneTree:   EBGT00050000008926
- HOGENOM:   HBG685300
- OMA:   LVRYTRF
- ProtClustDB:   PRK10592
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 30541; Mature: 30541

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1df4c86c)-; HASH(0x1df4a8dc)-; HASH(0x1eafa504)-; HASH(0x1f6617d0)-; HASH(0x1f0478d0)-; HASH(0x1f292f74)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157