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The map label for this gene is iaaA [H]

Identifier: 218688611

GI number: 218688611

Start: 823590

End: 824555

Strand: Direct

Name: iaaA [H]

Synonym: ECED1_0792

Alternate gene names: 218688611

Gene position: 823590-824555 (Clockwise)

Preceding gene: 218688608

Following gene: 218688612

Centisome position: 15.81

GC content: 56.31

Gene sequence:

>966_bases
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACG
CTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGTAGCGGGCGAAAGTGCGCTGGATGTGGCGA
CGGAAGCGGTGCGTCTGCTGGAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCTGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTTAGCCATCTGCGTAATCCGGT
TCTTGCCGCCCGACTGGTGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTC
ATGGCATGGAGTGCGTCTCGCCGGAGATTTTCTCCACACCTTTGCGTTATGAACAACTAATGGCAGCGCGCGAGGAAGGG
GCAACAGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAAAAAATGGGCACCGTAGGGGCCGTGGCGTTAGATTT
AGACGGCAATCTGGCGGCAGCCACGTCCACGGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAG
TGGGGGCCGGATGCTATGCCAATAACGCCAGTGTGGCGGTTTCCTGTACCGGCACGGGCGAAGTCTTCATCCGCGCGCTG
GCGGCATATGATATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAA
ACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAG
GAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAGAGGGGACACCGTTGCCACA
CAGTGA

Upstream 100 bases:

>100_bases
GCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTCATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAGCC
ACCCTTAATGGACGAATACT

Downstream 100 bases:

>100_bases
TGAACTTGATGCCGGTGATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGAGCAGCAGAAAATAGCTGCGG
TCCGCAATCTCTCTTTTAGC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLVAGESALDVATEAVRLLEECPLFNAGIGAVFTRDETH
ELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYRERGDTVAT
Q

Sequences:

>Translated_321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLVAGESALDVATEAVRLLEECPLFNAGIGAVFTRDETH
ELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYRERGDTVAT
Q
>Mature_320_residues
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLVAGESALDVATEAVRLLEECPLFNAGIGAVFTRDETHE
LDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEGA
TVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRALA
AYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYRERGDTVATQ

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=301, Percent_Identity=37.2093023255814, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI145275200, Length=301, Percent_Identity=37.2093023255814, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=32.7137546468402, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI285002251, Length=257, Percent_Identity=28.7937743190661, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=97.5077881619938, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569239, Length=252, Percent_Identity=28.968253968254, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32564649, Length=311, Percent_Identity=25.4019292604502, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18921183, Length=318, Percent_Identity=35.5345911949685, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=30.9701492537313, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI221330102, Length=233, Percent_Identity=33.0472103004292, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24652135, Length=233, Percent_Identity=33.0472103004292, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33321; Mature: 33190

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLVAGESALDVATEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEGATVLDHSGAPLDEKQKMGTV
EEEECCCCHHHHHCCCHHCCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYRERGDTVATQ
CCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure 
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLVAGESALDVATEAVRLL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEGATVLDHSGAPLDEKQKMGTV
EEEECCCCHHHHHCCCHHCCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYRERGDTVATQ
CCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]