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The map label for this gene is prpR [H]

Identifier: 218688206

GI number: 218688206

Start: 378038

End: 379624

Strand: Reverse

Name: prpR [H]

Synonym: ECED1_0363

Alternate gene names: 218688206

Gene position: 379624-378038 (Counterclockwise)

Preceding gene: 218688213

Following gene: 218688204

Centisome position: 7.29

GC content: 55.14

Gene sequence:

>1587_bases
ATGGCACATCCACCACGGCTGAATGACGACAAACCGGTTATCTGGACGGTATCTGTAACGCGGCTGTTCGAGCTGTTTCG
CGATATCAGCCTCGAGTTTGATCATCTGGCGAACATCACCCCTATTCAGCTTGGCTTTGAAAAAGCGGTGGCCTACATCC
GCAAAAAACTGGCCAGCGAACGCTGCGACGCCATCATTGCCGCTGGCTCTAACGGTGCGTACCTGAAAAGCCGCCTGTCG
GTACCGGTGATTCTGATAAAACCGAGTGGCTACGATGTGTTACAGGCGCTGGCAAAAGCCGGAAAACTCACCTCTTCTAT
CGGCGTTGTCACCTATCAGGAAACCATTCCGGCACTGGTAGCGTTTCAAAAAACCTTTAATTTGCGCCTCGACCAACGTA
GCTACATTACCGAAGAAGACGCACGCGGGCAGATTAACGAGCTAAAAGCCAACGGCACCGAAGCGGTGGTCGGCGCGGGG
CTGATTACCGATCTGGCAGAAGAAGCCGGAATGACCGGAATTTTTATCTATTCTACCGCCACCGTGCGCCAGGCATTTAG
CGATGCGCTGGATATGACGCGCATGTCTCTACGGCATAACACTCACGATGCCACCCGCAACGCCCTGCGTACTCGTTACG
TGCTGGGCGATATGCTCGGTCAATCACCACAGATGGAACAGGTGCGGCAGACTATTTTGCTGTATGCCCGCTCCAGTGCG
GCGGTGTTGATTGAGGGGGAAACGGGGACGGGCAAAGAGCTGGCGGCCCAGGCGATTCATCGGGAATATTTTTCCCGCCA
CGATGCGCGACAGGGCAAAAAGTCACATCCGTTTGTTGCCGTTAACTGCGGAGCGATTGCCGAATCGCTGCTGGAAGCGG
AGCTGTTTGGCTATGAGGAAGGGGCGTTTACCGGCTCGCGACGCGGAGGACGCGCCGGGCTGTTTGAAATCGCCCACGGT
GGCACGCTGTTTCTTGACGAGATAGGCGAAATGCCGCTGCCGTTGCAGACTCGGCTGCTGCGGGTGCTGGAAGAAAAAGA
GGTCACCCGCGTCGGCGGGCATCAGCCTGTTCCGGTGGATGTGCGGGTCATTAGCGCCACTCACTGCAATCTGGAAGAAG
ATATGCAGCAAGGGCAGTTTCGCCGTGACCTGTTTTATCGGCTGAGTATTTTGCGTCTACAACTGCCGCCGCTACGCGAG
CGGGTGGCGGATATTCTGCCGCTGGCGGAAAGCTTTTTGAAAATGTCTCTGGCGGCACTCTCTGTCCCTTTTTCTGCCGC
ATTACGTCAGGGATTAGAGACCTGTCAGATTGTATTATTGCTCTACGACTGGCCGGGCAATATTCGTGAACTGCGCAATA
TGATGGAACGACTGGCGTTATTTTTAAGTGTGGAACCGACGCCGGATTTAACGCCGCAATTTTTGCAGCTGCTACTGCCG
GAACTGGCGCGCGAGTCGGCGAAGACTCCCATTCAAGGCTTGCTGACAGCACAACAGGCACTGGAGAAATTTAATGGCGA
TAAGACAGCAGCGGCGAATTATTTAGGTATCAGCCGGACGACGTTCTGGCGGCGGCTGAAAAGCTGA

Upstream 100 bases:

>100_bases
TTTCAGTTAACGTTTCAGGCAATGTTTCACCGCGTTTCATTGCAACAATTATGAAACAAGACTAAACCCAATATTCGGTT
TCTTAACTTTGCGGTGCGCT

Downstream 100 bases:

>100_bases
ACTGCCGAATGCAGCGCGAACGCTTTATCCGGCATAAATTTCGATAGATGATGATCACCGATAAACTTACGCATCGGGGA
TGTTTACATAGGATTCAGAA

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSTATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKMSLAALSVPFSAALRQGLETCQIVLLLYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAKTPIQGLLTAQQALEKFNGDKTAAANYLGISRTTFWRRLKS

Sequences:

>Translated_528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSTATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKMSLAALSVPFSAALRQGLETCQIVLLLYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAKTPIQGLLTAQQALEKFNGDKTAAANYLGISRTTFWRRLKS
>Mature_527_residues
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLSV
PVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGL
ITDLAEEAGMTGIFIYSTATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSAA
VLIEGETGTGKELAAQAIHREYFSRHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGG
TLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILRLQLPPLRER
VADILPLAESFLKMSLAALSVPFSAALRQGLETCQIVLLLYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLPE
LARESAKTPIQGLLTAQQALEKFNGDKTAAANYLGISRTTFWRRLKS

Specific function: Involved in the transcriptional regulation of the propionate catabolism operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=528, Percent_Identity=96.5909090909091, Blast_Score=1043, Evalue=0.0,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=43.952802359882, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788550, Length=339, Percent_Identity=42.7728613569322, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI87082117, Length=355, Percent_Identity=42.2535211267606, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790437, Length=326, Percent_Identity=43.558282208589, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=38.0664652567976, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1788905, Length=376, Percent_Identity=38.8297872340425, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=38.5542168674699, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87082152, Length=311, Percent_Identity=40.5144694533762, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=43.5684647302905, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787583, Length=236, Percent_Identity=44.4915254237288, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=333, Percent_Identity=30.03003003003, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=34.9794238683128, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR012704
- InterPro:   IPR010524 [H]

Pfam domain/function: PF02954 HTH_8; PF06506 PrpR_N; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58635; Mature: 58504

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASE
CCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSTA
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMQQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKMSLAAL
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVPFSAALRQGLETCQIVLLLYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
CCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKTPIQGLLTAQQALEKFNGDKTAAANYLGISRTTFWRRLKS
HHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASE
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSTA
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMQQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKMSLAAL
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVPFSAALRQGLETCQIVLLLYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
CCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKTPIQGLLTAQQALEKFNGDKTAAANYLGISRTTFWRRLKS
HHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]