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The map label for this gene is yafC [H]

Identifier: 218688078

GI number: 218688078

Start: 234693

End: 235607

Strand: Reverse

Name: yafC [H]

Synonym: ECED1_0213

Alternate gene names: 218688078

Gene position: 235607-234693 (Counterclockwise)

Preceding gene: 218688081

Following gene: 218688075

Centisome position: 4.52

GC content: 49.07

Gene sequence:

>915_bases
ATGAAAGCCACGTCGGAAGAACTCGCCATTTTTGTTTCAGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCGGTGAAAAAGCTGGAGATGAAACTTGGCGTTAGCCTGCTTAATCGGACCA
CGCGGCAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCATCGCGTACAGTCAATTTTGCAGGAGATGGCAGCGGCA
GAATCAGAAATTATGGAGACGCGTAATACACCGCGTGGACTGTTACGGATCGATGCCGCAACTCCAGTGGTGCTGCACTT
TCTGATGCCGTTGATTAAGCCTTTTCGTGAACGCTATCCGGAAGTCACTTTATCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATCGAAAGAAAAGTGGATGTCGCGATACGCGCCGGTACGTTAACGGATTCCAGCTTACGTGCCAGGCCGTTATTT
AACAGTTATCGAAAAATTATCGCCTCCCCCGATTATATTTCCTGCTACGGGAAGCCAGAAACGATCGACGATTTAAAGCA
ACATATTTGCCTGAGATTCACTGAACCCGCTTCCCTCAATACCTGGCCGATAGCCTGTAGCGATGGACAATTACATGAGG
TGAAGTACGGTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTACATGATCGACAAAGAAATCGCTCGCGGAGAATTGGTGGAGTTAATGGCAGATAAAGTGTTGCCAGTGGAAATGCC
ATTCAGTGCAGTCTATTACAGCGACCGTGCGGTAAGTACGCGCATCCGGGCTTTTATCGATTTCCTTAGCGGACATGTAA
AAACAGCTCCCGGAGGAGCTGTCAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTATTGAGAACATTAAATCGGATGGCATATCTCGCATCCTGATGGTTTAATGACAAATAAATTCAAAATATCTCTTCT
ATTTTGCAACAGGAGCAAAA

Downstream 100 bases:

>100_bases
TCCCATTCAGGAGCCAGACCTTCCGGGCTAACCAGGCGGTCGTTGCAATCCAGTGCGGCGATCGCTTTTTTATCTTCGGC
ATCAAGCTGTAAATTTTGTG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSGHVKTAPGGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSGHVKTAPGGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSGHVKTAPGGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=98.3552631578947, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=33.9160839160839, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.6928327645051, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.3783783783784, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=31.4878892733564, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=28.1879194630872, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.125, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=28.169014084507, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=35, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=25.503355704698, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=24.3150684931507, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=28.0936454849498, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=29.0909090909091, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=266, Percent_Identity=25.9398496240602, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=81, Percent_Identity=39.5061728395062, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789506, Length=193, Percent_Identity=27.4611398963731, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=201, Percent_Identity=24.3781094527363, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=24.9158249158249, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33678; Mature: 33678

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECH
EEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISCYGKPE
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
TIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHEEECCCCCCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSGHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECH
EEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISCYGKPE
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
TIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHEEECCCCCCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSGHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]