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The map label for this gene is folK [H]

Identifier: 218688019

GI number: 218688019

Start: 163254

End: 163733

Strand: Reverse

Name: folK [H]

Synonym: ECED1_0147

Alternate gene names: 218688019

Gene position: 163733-163254 (Counterclockwise)

Preceding gene: 218688020

Following gene: 218688018

Centisome position: 3.14

GC content: 49.17

Gene sequence:

>480_bases
ATGACAGTGGCGTATATTGCCATAGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCTGCCCTGAAAGCATTAGG
CGATATTCCAGAAAGCCGCATTCTTGCTGTTTCTTCGTTTTACCGCACCCCACCGCTGGGGCCGCAAGATCAACCCGATT
ACTTAAATGCCGCCGTCGCGCTGAAAACCACTCTTGCCCCTGAAGAGCTACTCAATCACACACAACGTATCGAATTGCAG
CAAGGTCGCGTCCGTAAAGCTGAACGCTGGGGACCGCGCACGCTGGATCTCGACATCATGCTGTTTGGTAATGAAGTAAT
AAATACCGAACGTCTGACCGTTCCGCACTACGATATGAAAAATCGCGGCTTTATGCTGTGGCCACTGTTTGAAATTGCGC
CGGAATTGGTATTTCCTGATGGCTTATCATTAGTTGAAGCTCTACAGGCAAAAGGCTTTAATGAATTAGATAAGTGGTAA

Upstream 100 bases:

>100_bases
CAGACCAAAAAGGGATGCTTAACGAACTGGATGAAGAACCGTCACCGCGTCGTCGTACTCGTCGTCCACGCAAACGCGCA
CCGCGTCGTGAGGGTACCGC

Downstream 100 bases:

>100_bases
TTATTTTTCGTGTGAATATAAATATATTCCGAAAAATAACCTTAAGCTATTGCAAATAATAATATATTTAAGATAAATAA
AAACACAGAAAATATAATAT

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTPPLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW

Sequences:

>Translated_159_residues
MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTPPLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW
>Mature_158_residues
TVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTPPLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=89.937106918239, Blast_Score=296, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6324073, Length=142, Percent_Identity=33.8028169014084, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17843; Mature: 17712

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTPPLGPQDQPDYLNAAVA
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHCCCCCCCCCCCCHHHHHHH
LKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW
CCCEEEEEHHHHCHHHCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTPPLGPQDQPDYLNAAVA
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHCCCCCCCCCCCCHHHHHHH
LKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW
CCCEEEEEHHHHCHHHCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]