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The map label for this gene is ecpD [H]

Identifier: 218688017

GI number: 218688017

Start: 161451

End: 162191

Strand: Reverse

Name: ecpD [H]

Synonym: ECED1_0145

Alternate gene names: 218688017

Gene position: 162191-161451 (Counterclockwise)

Preceding gene: 218688018

Following gene: 218688016

Centisome position: 3.11

GC content: 44.26

Gene sequence:

>741_bases
ATGTTTTTTAATACTAAACATACCGCAGCATTGTGCTTAGCGAGCTGTATGGCTTTTAGCTCATCAGCTATTGCAGACAT
CGTAATTTCAGGTACACGCGTAGTTTATAAAAGCGAACAAAAAAGCGTCAATGTACGTCTGGAAAATAAAGGCAATAACC
CATTGCTGGTTCAGAGTTGGTTAGATACTGGAGATGATAACGCTGAACCAGGCAGTATTACTGTCCCCTTTACCGCTACG
CCACCAGTATCACGTATTGATGCTAAACGTGGCCAAACAATCAAACTGATGTACACCGCCAGTAGTGCTCTGCCAAAAGA
CAGAGAAAGTGTGTTCTGGTTTAACGTACTGGAAGTTCCACCAAAACCCGATGCAGCAAAAGCAGCAAATCAAAGCCTGC
TACAACTGGCATTTCGCACACGTATAAAACTGTTCTATCGCCCGGAAGGATTGAGTGGAATTTCATCTGATGCACCATTG
GCTCTGAAGTGGTCCTGGGCAACATCTGAAGGCAAAGCAGCATTACGCGTTGATAACCCTACGCCCTACTATGTCTCTTT
TAGCAGTGGTGATTTAGAAGCCAACGGCAAACGCTATCCCCTGGATATGACAATGATTGCGCCGTTCAGTAACGACGTCA
TCAAGGTAAATGGGATGAGTGGCAGAACAAGCTCTGCAAAAGTGCATTTTTTCGCCATTAATGATTTTGGTGGATCTATT
GAAGGCAACGCCAACCTTTAA

Upstream 100 bases:

>100_bases
CGCTTAAATTGATCGGGTGAATATATACAGGGAATAACATCAAATATTTTTACTTTTGATAATTATTCCCTGTTTTAATT
AATTCTGTAAGGAATGGTTT

Downstream 100 bases:

>100_bases
TCTGGCAAGGATATAACACAGCAGGAAGCAGAACGTGACTATTAAATCTACTAATCACCTTACCCACATCGCCACGTTTT
GCGCTCTGCTGTATAGCAAT

Product: putative chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSI
EGNANL

Sequences:

>Translated_246_residues
MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSI
EGNANL
>Mature_246_residues
MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSI
EGNANL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=85.3658536585366, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1788428, Length=240, Percent_Identity=34.1666666666667, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789532, Length=241, Percent_Identity=34.4398340248963, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1788677, Length=167, Percent_Identity=40.1197604790419, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1786936, Length=209, Percent_Identity=29.6650717703349, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790771, Length=231, Percent_Identity=29.4372294372294, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=30.1255230125523, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI87082203, Length=243, Percent_Identity=30.8641975308642, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787171, Length=227, Percent_Identity=29.5154185022026, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081806, Length=239, Percent_Identity=29.7071129707113, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26709; Mature: 26709

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVNVRLENKGNNPLLVQSW
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSALPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPLALKWSWATSEGKAALRVDNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
TPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCC
EGNANL
CCCCCC
>Mature Secondary Structure
MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVNVRLENKGNNPLLVQSW
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSALPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPLALKWSWATSEGKAALRVDNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
TPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCC
EGNANL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]