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The map label for this gene is garR [H]

Identifier: 218551582

GI number: 218551582

Start: 4475683

End: 4476573

Strand: Direct

Name: garR [H]

Synonym: EFER_4367

Alternate gene names: 218551582

Gene position: 4475683-4476573 (Clockwise)

Preceding gene: 218551581

Following gene: 218551585

Centisome position: 97.54

GC content: 50.39

Gene sequence:

>891_bases
ATGACGATGAAAGTTGGTTTTATTGGCCTTGGGATTATGGGTAAACCAATGAGTAAAAACCTCATTAAAGCAGGTTACTC
ACTGGTGGTTGCTGATCGTAACCCGCAAGCCATTGCCGAAGTTATAGAAGCTGGAGCAGAAACGGCGGCTACTGCCAAAG
CAATCGCTGAACAGTGTGATGTGATTATTACCATGCTGCCTAACTCCCCACATGTGAAAGAAGTTGCGCTCGGGGAAGGC
GGCATAATTGAAGGTGCAAAACCGGGTACAGTCCTGATCGATATGAGTTCTATTGCACCGCTGGCAAGCCGTGAAATTAG
CGAAGCACTTAAAGCGAAAGGTATCGAAATGCTGGATGCGCCGGTTAGTGGCGGAGAACCAAAAGCCATCGACGGAACGC
TTTCGGTGATGGTGGGGGGCGACAAGGCGATTTTCGATAAATATTACGATCTGATGAAAGCGATGGCGGGATCTGTGGTG
CATACCGGGGAAATCGGCGCGGGTAATGTCACCAAACTGGCAAATCAGGTGATTGTGGCACTGAACATTGCCGCGATGTC
AGAAGCATTAACACTGGCGACCAAAGCGGGTGTTAATCCTGATTTGGTCTATCAGGCAATCCGGGGTGGTCTGGCAGGTA
GCACCGTACTGGATGCGAAAGCGCCAATGGTCATGGATCGCAACTTCAAGCCAGGTTTCCGTATTGATCTGCACATTAAA
GATCTGGCGAATGCTCTGGATACTTCTCATGGTGTGGGGGCACAGCTGCCATTAACGGCAGCGGTGATGGAAATGATGCA
AGCGCTGCGCGCAGATGGTCTTGGAACTGCGGATCACAGTGCTCTGGCCTGCTATTACGAAAAAATGGCGAAAGTGGAAG
TGGCTCGCTAA

Upstream 100 bases:

>100_bases
ATTTGTTGCTGTAGGTAGCGACCTTGGGGTTTTCCGCACGGGTACTCAACGTTTGGCTGATTCCTTTAAAAAATAACCAC
GAGTGAAGAGAGAAAACGAT

Downstream 100 bases:

>100_bases
TGATGTTACGCCTGGCGGCTCGCAAAAGCGGCTAAGTAAAACGCCATCGGCATTGGGTGGTAACATATTTACCGGACTTT
GTGCAATATTGCCGTCACGT

Product: tartronate semialdehyde reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTMKVGFIGLGIMGKPMSKNLIKAGYSLVVADRNPQAIAEVIEAGAETAATAKAIAEQCDVIITMLPNSPHVKEVALGEG
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIEMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKMAKVEVAR

Sequences:

>Translated_296_residues
MTMKVGFIGLGIMGKPMSKNLIKAGYSLVVADRNPQAIAEVIEAGAETAATAKAIAEQCDVIITMLPNSPHVKEVALGEG
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIEMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKMAKVEVAR
>Mature_295_residues
TMKVGFIGLGIMGKPMSKNLIKAGYSLVVADRNPQAIAEVIEAGAETAATAKAIAEQCDVIITMLPNSPHVKEVALGEGG
IIEGAKPGTVLIDMSSIAPLASREISEALKAKGIEMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVH
TGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKD
LANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKMAKVEVAR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=35.0877192982456, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=28.7234042553192, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=96.9387755102041, Blast_Score=572, Evalue=1e-165,
Organism=Escherichia coli, GI1786719, Length=293, Percent_Identity=43.6860068259386, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=36.0714285714286, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=34.7517730496454, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=33.8181818181818, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=32.5423728813559, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=32.5423728813559, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=26.241134751773, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30660; Mature: 30529

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKVGFIGLGIMGKPMSKNLIKAGYSLVVADRNPQAIAEVIEAGAETAATAKAIAEQCD
CEEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGEGGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIEMLDA
EEEEECCCCCCCEEEECCCCCEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCEEEEC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKMAKVEVAR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMKVGFIGLGIMGKPMSKNLIKAGYSLVVADRNPQAIAEVIEAGAETAATAKAIAEQCD
EEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGEGGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIEMLDA
EEEEECCCCCCCEEEECCCCCEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCEEEEC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKMAKVEVAR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]