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The map label for this gene is hflK [H]

Identifier: 218551444

GI number: 218551444

Start: 4335370

End: 4336629

Strand: Direct

Name: hflK [H]

Synonym: EFER_4227

Alternate gene names: 218551444

Gene position: 4335370-4336629 (Clockwise)

Preceding gene: 218551443

Following gene: 218551445

Centisome position: 94.48

GC content: 55.71

Gene sequence:

>1260_bases
ATGGCGTGGAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGG
AAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTG
GTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGTAATTCATCGCAAGGCCCGCGCCCGCAGCTTGGCGGTCGTGTC
GTTACCATCGCAGCGGCAGCGATTGTCATTATTTGGGCGGCCAGTGGTTTCTACACCATTAAAGAAGCCGAACGCGGCGT
GGTAACACGCTTTGGTAAATTCAGCCATCTGGTTGAGCCGGGTCTGAACTGGAAACCGACGTTTATCGACGAAGTCAAAC
CGGTGAACGTGGAAGCCGTGCGTGAACTGGCTGCTTCCGGTGTGATGCTGACGTCGGACGAAAACGTGGTGCGCGTTGAG
ATGAACGTACAGTACCGCGTCACCAATCCGGAAAAATATCTATATAGCGTGACCAGCCCGGATGACAGCCTGCGTCAGGC
TACCGACAGCGCCCTGCGTGGGGTTATCGGTAAATACACCATGGACCGCATTCTGACGGAAGGTCGTACCGTGATTCGTA
GCGATACTCAGCGCGAACTGGAAGAGACGATTCGTCCGTATGACATGGGTATCACTCTGCTGGACGTCAACTTCCAGGCA
GCTCGTCCGCCGGAAGAAGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGA
AGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCTCGTGCGTACA
AGGCTCAGACTATCCTGGAAGCCCAGGGTGAAGTGGCACGCTTTGCTAAACTTCTGCCGGAATATAAAGCTGCGCCGGAA
ATTACCCGCGAGCGCCTGTATATCGAGACGATGGAAAAAGTGTTGGGCAACACCCGCAAAGTGCTGGTTAACGATAAAGG
TGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGCAATGCCCCTGCGGCGAAGAGCGATAACGGTGCCA
GCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAT
ATTATGGACCAACGCCGCGCTAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAA

Upstream 100 bases:

>100_bases
GATTACTTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCAT
AACAAATATGGAGCACAAAC

Downstream 100 bases:

>100_bases
CGATGCGTAAGTCAGTTATCGCGATTATCATCATCGTGCTGGTAGTGCTTTACATGTCTGTCTTTGTCGTCAAAGAAGGT
GAGCGCGGTATTACGCTGCG

Product: FtsH protease regulator HflK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGNSSQGPRPQLGGRV
VTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPE
ITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGD
IMDQRRANAQRNDYQRQGE

Sequences:

>Translated_419_residues
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGNSSQGPRPQLGGRV
VTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPE
ITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGD
IMDQRRANAQRNDYQRQGE
>Mature_418_residues
AWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGNSSQGPRPQLGGRVV
TIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEM
NVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQAA
RPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEI
TRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDI
MDQRRANAQRNDYQRQGE

Specific function: HflC and HflK govern the stability of phage lambda cII protein and have been proposed to encode or regulate a cII- specific protease [H]

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the band 7/mec-2 family. HflK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790616, Length=419, Percent_Identity=99.7613365155131, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1786697, Length=281, Percent_Identity=22.7758007117438, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001107
- InterPro:   IPR010201
- InterPro:   IPR020980
- InterPro:   IPR001972 [H]

Pfam domain/function: PF01145 Band_7; PF12221 HflK_N [H]

EC number: NA

Molecular weight: Translated: 45572; Mature: 45441

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TGSGGGNSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHCC
GLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSP
CCCCCCCHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEEECHHHEEEEECCC
DDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCHHHCCEEEEEECCCC
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLK
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHC
GGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
AWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TGSGGGNSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHCC
GLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSP
CCCCCCCHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEEECHHHEEEEECCC
DDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCHHHCCEEEEEECCCC
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLK
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHC
GGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]