Click here to switch to the map view.

The map label for this gene is poxA [H]

Identifier: 218551427

GI number: 218551427

Start: 4315385

End: 4316362

Strand: Direct

Name: poxA [H]

Synonym: EFER_4209

Alternate gene names: 218551427

Gene position: 4315385-4316362 (Clockwise)

Preceding gene: 218551420

Following gene: 218551428

Centisome position: 94.04

GC content: 50.72

Gene sequence:

>978_bases
ATGAGCGAAACGGCAACCTGGCAGCCGAGCGCATCCATCCCAAACCTTTTAAAACGTGCAGCGATTATGACGGAAATTCG
GCGTTTCTTTGCCGATCGCGGCGTACTGGAGGTGGAAACGCCTTGTATGAGCCAGGCAACGGTTACAGATATTCATCTCG
TCCCGTTTGAAACGCGTTTCGTAGGCCCTGGGCACTCTCAGGGGATGAATCTTTGGTTAATGACCAGCCCGGAATACCAC
ATGAAACGCTTGCTGGTAGCCGGTTGCGGGCCAATCTTTCAGTTGTGTCGTAGTTTCCGCAATGAAGAGATGGGGCGACA
TCACAATCCTGAATTCACCATGCTGGAGTGGTATCGTCCCCACTATGACATGTATCGACTGATGAATGAAGTGGATGATC
TACTGCAACAAGTTCTTGATTGTGCCCCTGCGGAAAGTCTTTCATATCAGCAGGCCTTCCAGCGTTATCTGGAGATTGAT
CCTCTCTCTGCCGACAAAGCGCAGCTTCGGGAAGCGGCAGCAAAACTCGATTTGAGTAACGTGGCTGACGAAGAAGAAGA
TCGTGACACTTTGCTACAACTGCTCTTTACCTTTGGTGTAGAACCAAATATTGGTAAAGAAAAACCGACATTTGTGTATC
ATTTTCCGGCCAGTCAGGCATCACTGGCGCAAATCAGTACCGAAGATCATCGGGTCGCTGAACGCTTTGAGGTTTACTAT
AAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTAACGGATGCTCGTGAACAAGAGCAACGATTCGAGCAGGATAACCG
CAAACGTGCCGCGCGCGGGTTACCGCAGCATCCTATTGATCATAACCTGATTGAGGCGCTGAAAGTTGGGATGCCTGACT
GCTCTGGCGTGGCGCTGGGGGTGGATCGCCTGGTCATGCTGGCGTTGGGGGCTGAGCGTTTGTCTGACGTTATTGCCTTT
AGTGTCGATCGGGCGTAA

Upstream 100 bases:

>100_bases
TTAATCTTTCTTTTTCATCTGAATAATAACGTGCAAAATTTGTCTCCCCGACGCGATACGAGTAGACTTCCTGCCCTTGT
CATAAACAGGAGAAAGCATC

Downstream 100 bases:

>100_bases
TTCCGAAAATCACTTCATTAAGAGACAAAATGGCGTTTTGAACGCCATTTTGTAGCATATATTTTCATTTTCGCGCGAAA
AGCCACAATTTCAGGCTGGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETATWQPSASIPNLLKRAAIMTEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYH
MKRLLVAGCGPIFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCAPAESLSYQQAFQRYLEID
PLSADKAQLREAAAKLDLSNVADEEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQEQRFEQDNRKRAARGLPQHPIDHNLIEALKVGMPDCSGVALGVDRLVMLALGAERLSDVIAF
SVDRA

Sequences:

>Translated_325_residues
MSETATWQPSASIPNLLKRAAIMTEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYH
MKRLLVAGCGPIFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCAPAESLSYQQAFQRYLEID
PLSADKAQLREAAAKLDLSNVADEEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQEQRFEQDNRKRAARGLPQHPIDHNLIEALKVGMPDCSGVALGVDRLVMLALGAERLSDVIAF
SVDRA
>Mature_324_residues
SETATWQPSASIPNLLKRAAIMTEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHM
KRLLVAGCGPIFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCAPAESLSYQQAFQRYLEIDP
LSADKAQLREAAAKLDLSNVADEEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYYK
GIELANGFHELTDAREQEQRFEQDNRKRAARGLPQHPIDHNLIEALKVGMPDCSGVALGVDRLVMLALGAERLSDVIAFS
VDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=338, Percent_Identity=26.3313609467456, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI5031815, Length=338, Percent_Identity=26.3313609467456, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=95.3846153846154, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=324, Percent_Identity=30.2469135802469, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789256, Length=324, Percent_Identity=30.2469135802469, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994340, Length=340, Percent_Identity=29.1176470588235, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17535925, Length=340, Percent_Identity=29.1176470588235, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17535927, Length=340, Percent_Identity=29.1176470588235, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320242, Length=332, Percent_Identity=27.1084337349398, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324256, Length=348, Percent_Identity=27.0114942528736, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640849, Length=335, Percent_Identity=27.1641791044776, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640851, Length=335, Percent_Identity=27.1641791044776, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37061; Mature: 36929

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETATWQPSASIPNLLKRAAIMTEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCEE
VGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPIFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCAPAESLSYQQAFQRYLEIDPLSADKAQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHH
VADEEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQEQRFEQDNRKRAARGLPQHPIDHNLIEALKVGMPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHH
VDRLVMLALGAERLSDVIAFSVDRA
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SETATWQPSASIPNLLKRAAIMTEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCEE
VGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPIFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCAPAESLSYQQAFQRYLEIDPLSADKAQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHH
VADEEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQEQRFEQDNRKRAARGLPQHPIDHNLIEALKVGMPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHH
VDRLVMLALGAERLSDVIAFSVDRA
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA