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The map label for this gene is spoT [H]

Identifier: 218551177

GI number: 218551177

Start: 4039293

End: 4041401

Strand: Direct

Name: spoT [H]

Synonym: EFER_3942

Alternate gene names: 218551177

Gene position: 4039293-4041401 (Clockwise)

Preceding gene: 218551176

Following gene: 218551178

Centisome position: 88.03

GC content: 53.25

Gene sequence:

>2109_bases
TTGTATCTGTTTGAAAGCCTGAATCAGCTGATTCAAAACTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTA
TCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTGGCAGTTGCCT
GCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTAATGGCAGCACTGCTGCATGACGTGATTGAAGATACACCCGCC
ACCTACCAGGACATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAACTGGTAGAGGGGGTATCAAAGCTTGATAAACTCAA
GTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGACATCCGCGTCATTC
TCATCAAACTTGCTGACCGTACCCACAATATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGT
GAAACACTCGAAATTTACAGCCCGCTGGCACACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTT
TGAAGCGCTGTATCCCAACCGTTACCGCGTGATTAAAGAAGTGGTGAAAGCCGCGCGTGGCAACCGTAAAGAGATGATCC
AAAAAATCCTCTCGGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTT
TATTCGATCTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCCTTCCGCGTGATCGT
CAATGATTCCGACACCTGTTATCGCGTGCTCGGCCAAATGCACAGCCTGTATAAGCCGCGTCCGGGTCGCGTGAAAGACT
ACATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTC
CAGATCCGTACCGAAGATATGGATCAGATGGCAGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAC
CAGTACTACCGCACAAATTCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGTGCCGGTAGCTCATTTG
AATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTAGAGCTG
CCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCGGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCTCGCGTCGA
CCGTCAGCCTTACCCGCTGTCGCAGCCGCTCTCCAGTGGCCAAACCGTTGAAATCATTACCGCACCGGGCGCTCGCCCGA
ATGCCGCATGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGAT
GATTCTGTAAGCCTGGGCCGTCGTCTGCTCAATCACGCGTTGGGTGGCAGCCGTAAGCTCAATGAAATCCCGCCGGAAAA
TATTCAGCGCGAGCTGGACCGCATGAAGCTGGTAACGCTTGACGATCTGCTGGCGGAAATTGGTCTTGGCAACGCGATGA
GCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGTGACTCAAAGCCACGGGCATCTGCCGATT
AAAGGTGCTGATGGCGTGTTGATCACCTTTGCGAAATGCTGCCGCCCCATTCCTGGCGATCCGATTATCGCTCACGTCAG
CCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAACATCCGTGGCTACCAGAAAGAGCCAGAGAAATTTATGG
CTGTAGAATGGGATAAAGAGACGGCACAGGAGTTCATCACCGAGATCAAAGTGGAGATGTTCAATCATCAGGGGGCATTA
GCTAACCTGACGGCAGCGATCAATACGACCAACTCCAATATTCAAAGTTTGAATACAGAAGAAAAGGATGGTCGCGTCTA
CAGCGCCTTTATTCGTCTGACCGCCCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACG
TGATTAAAGTCACCCGAAACCGAAATTAA

Upstream 100 bases:

>100_bases
CGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTT
AATCACAAAGCGGGTCGCCC

Downstream 100 bases:

>100_bases
TGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGA
GCAGGTCCACAAACCACATA

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLNEIPPENIQRELDRMKLVTLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPVTQSHGHLPI
KGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGAL
ANLTAAINTTNSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Sequences:

>Translated_702_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLNEIPPENIQRELDRMKLVTLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPVTQSHGHLPI
KGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGAL
ANLTAAINTTNSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
>Mature_702_residues
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLNEIPPENIQRELDRMKLVTLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPVTQSHGHLPI
KGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGAL
ANLTAAINTTNSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=702, Percent_Identity=99.1452991452991, Blast_Score=1448, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=680, Percent_Identity=31.7647058823529, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650996, Length=118, Percent_Identity=40.6779661016949, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79381; Mature: 79381

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEHHCCCCHH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DSVSLGRRLLNHALGGSRKLNEIPPENIQRELDRMKLVTLDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LQHGDASIPPVTQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCR
CCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHHC
NIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTNSNIQSLNTE
CCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHCCCC
EKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEHHCCCCHH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DSVSLGRRLLNHALGGSRKLNEIPPENIQRELDRMKLVTLDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LQHGDASIPPVTQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCR
CCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHHC
NIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTNSNIQSLNTE
CCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHCCCC
EKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]