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The map label for this gene is rfaL [H]

Identifier: 218551150

GI number: 218551150

Start: 4012443

End: 4013660

Strand: Direct

Name: rfaL [H]

Synonym: EFER_3911

Alternate gene names: 218551150

Gene position: 4012443-4013660 (Clockwise)

Preceding gene: 218551149

Following gene: 218551156

Centisome position: 87.44

GC content: 35.8

Gene sequence:

>1218_bases
ATGTTAACAGCCTCGCTGGCGCTACGTAATAAAGAAAACTGGAAACCGCACTGGAATAAAGCGTTGGTATTCCTCTTTAT
TGCAACCTTTTTCCTTGATGGTATAACCCGTTATAAACACATCATATCTATACTAATGATTATTACGGTAATCTATCAAG
TTTCCCGTGCTCCAGGAACATTTAAAGTACTCTATAAAAATAATCTTTTTTATAGTGTCCTGGTACTATCACTCATTCTT
TTATATGCTACTTTCATATCACCTGATCTGAAAATTAGTTTTAAAGAATTCAGTAATACGGTGCTTAAAGGATTTTTGGC
ATATAGTTTACTTATACCCGCATTATTAAAAGATGAAGATAATGAAAACATCGGTAAAATTGTACTATACTCATTAGTTA
CCGGACTTGGGTTACGTTGTCTTGTTGAACTTATTCTTTATATTCAGGATTATAATAAAGGAATAATGCCATTTTCCACC
TATGAACACCGTAGTATTTCCGATTCAATGGTGTTTCTGTTTCCAGCATTATTAAACCTTTGGCTTATCAAGAAAACCTC
TTATAAAATAGCATTCGTAATTTTTAGTGGAGTTTTCTTATTTCTGCTATTAGGAACGCTGTCTCGTGGTGCATGGCTGG
CAGTATTCATAGTGACTCTGCTATGGTTAATCTTAAATCGTCAGTGGAAATTATTGATGCTGACTTCCATCGTTATTTCT
GTTGCCGCAGCAGGTGTATTTACCTATAAAGGTGGTCATGCCGGTAAAGACAGGCTTATTTATAAACTTCAACAGACCGA
CAGTTCTTATCGCTATACCAATGGTACACAAGGTACTGCGTGGACATTAATTATGGAAAATCCGCTTAAGGGATATGGCT
ACGGCGATGATATTTACCATGCAATATATAATAAGCGTGTCGTAGATTTTCCGTCATGGACGTTCAGGCAATCCATTGGA
CCACACAACGTTGTTTTGTCAATTTGGTTTGCTGCAGGTTTAGCAGGACTACTCGCTTTACTTTATCTGTATGGCTCTAT
TATCAAAGAAACAGCTAATGCAACATTTAAAAAAGTTGTGGTCACTCCTTATAATGGTCAATTGCTACTATTCTTAACGC
TCATCGGTTTTTATATTATTCGAGGTAACTTCGAAGAAGTTGATCTTAAACCAATTGGCTTGATTGTTGGTTTACTGTTA
GCAATGCGGAATAAATAA

Upstream 100 bases:

>100_bases
CGCCAGATAAAGATCTTGCTTATCTGACTGCAGAAACGGTATTTAATAAAATCAATTCCTGATTAATACGTTATATATTA
TTTAAGATGGTGGAGAAGCA

Downstream 100 bases:

>100_bases
AAAAAGCCTGCCTGACATTAAATTCAGGCAGGCTCTTCAAATCCAATAATTTTTAGCCAAACCAGTCTTGTATTTGTGCT
TCAAAACGCTGAGTAACATG

Product: O-antigen ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLTASLALRNKENWKPHWNKALVFLFIATFFLDGITRYKHIISILMIITVIYQVSRAPGTFKVLYKNNLFYSVLVLSLIL
LYATFISPDLKISFKEFSNTVLKGFLAYSLLIPALLKDEDNENIGKIVLYSLVTGLGLRCLVELILYIQDYNKGIMPFST
YEHRSISDSMVFLFPALLNLWLIKKTSYKIAFVIFSGVFLFLLLGTLSRGAWLAVFIVTLLWLILNRQWKLLMLTSIVIS
VAAAGVFTYKGGHAGKDRLIYKLQQTDSSYRYTNGTQGTAWTLIMENPLKGYGYGDDIYHAIYNKRVVDFPSWTFRQSIG
PHNVVLSIWFAAGLAGLLALLYLYGSIIKETANATFKKVVVTPYNGQLLLFLTLIGFYIIRGNFEEVDLKPIGLIVGLLL
AMRNK

Sequences:

>Translated_405_residues
MLTASLALRNKENWKPHWNKALVFLFIATFFLDGITRYKHIISILMIITVIYQVSRAPGTFKVLYKNNLFYSVLVLSLIL
LYATFISPDLKISFKEFSNTVLKGFLAYSLLIPALLKDEDNENIGKIVLYSLVTGLGLRCLVELILYIQDYNKGIMPFST
YEHRSISDSMVFLFPALLNLWLIKKTSYKIAFVIFSGVFLFLLLGTLSRGAWLAVFIVTLLWLILNRQWKLLMLTSIVIS
VAAAGVFTYKGGHAGKDRLIYKLQQTDSSYRYTNGTQGTAWTLIMENPLKGYGYGDDIYHAIYNKRVVDFPSWTFRQSIG
PHNVVLSIWFAAGLAGLLALLYLYGSIIKETANATFKKVVVTPYNGQLLLFLTLIGFYIIRGNFEEVDLKPIGLIVGLLL
AMRNK
>Mature_405_residues
MLTASLALRNKENWKPHWNKALVFLFIATFFLDGITRYKHIISILMIITVIYQVSRAPGTFKVLYKNNLFYSVLVLSLIL
LYATFISPDLKISFKEFSNTVLKGFLAYSLLIPALLKDEDNENIGKIVLYSLVTGLGLRCLVELILYIQDYNKGIMPFST
YEHRSISDSMVFLFPALLNLWLIKKTSYKIAFVIFSGVFLFLLLGTLSRGAWLAVFIVTLLWLILNRQWKLLMLTSIVIS
VAAAGVFTYKGGHAGKDRLIYKLQQTDSSYRYTNGTQGTAWTLIMENPLKGYGYGDDIYHAIYNKRVVDFPSWTFRQSIG
PHNVVLSIWFAAGLAGLLALLYLYGSIIKETANATFKKVVVTPYNGQLLLFLTLIGFYIIRGNFEEVDLKPIGLIVGLLL
AMRNK

Specific function: Adds the O-antigen on the glucose(II) group of LPS [H]

COG id: COG3307

COG function: function code M; Lipid A core - O-antigen ligase and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the O-antigen polymerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007016 [H]

Pfam domain/function: PF04932 Wzy_C [H]

EC number: NA

Molecular weight: Translated: 45951; Mature: 45951

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTASLALRNKENWKPHWNKALVFLFIATFFLDGITRYKHIISILMIITVIYQVSRAPGT
CCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKVLYKNNLFYSVLVLSLILLYATFISPDLKISFKEFSNTVLKGFLAYSLLIPALLKDED
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
NENIGKIVLYSLVTGLGLRCLVELILYIQDYNKGIMPFSTYEHRSISDSMVFLFPALLNL
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WLIKKTSYKIAFVIFSGVFLFLLLGTLSRGAWLAVFIVTLLWLILNRQWKLLMLTSIVIS
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAAGVFTYKGGHAGKDRLIYKLQQTDSSYRYTNGTQGTAWTLIMENPLKGYGYGDDIYH
HHHHHHEEECCCCCCCHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHH
AIYNKRVVDFPSWTFRQSIGPHNVVLSIWFAAGLAGLLALLYLYGSIIKETANATFKKVV
HHHCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VTPYNGQLLLFLTLIGFYIIRGNFEEVDLKPIGLIVGLLLAMRNK
EECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTASLALRNKENWKPHWNKALVFLFIATFFLDGITRYKHIISILMIITVIYQVSRAPGT
CCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKVLYKNNLFYSVLVLSLILLYATFISPDLKISFKEFSNTVLKGFLAYSLLIPALLKDED
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
NENIGKIVLYSLVTGLGLRCLVELILYIQDYNKGIMPFSTYEHRSISDSMVFLFPALLNL
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WLIKKTSYKIAFVIFSGVFLFLLLGTLSRGAWLAVFIVTLLWLILNRQWKLLMLTSIVIS
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAAGVFTYKGGHAGKDRLIYKLQQTDSSYRYTNGTQGTAWTLIMENPLKGYGYGDDIYH
HHHHHHEEECCCCCCCHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHH
AIYNKRVVDFPSWTFRQSIGPHNVVLSIWFAAGLAGLLALLYLYGSIIKETANATFKKVV
HHHCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VTPYNGQLLLFLTLIGFYIIRGNFEEVDLKPIGLIVGLLLAMRNK
EECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1657881; 11677609 [H]