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The map label for this gene is leuC1 [H]

Identifier: 218550939

GI number: 218550939

Start: 3752769

End: 3754715

Strand: Direct

Name: leuC1 [H]

Synonym: EFER_3665

Alternate gene names: 218550939

Gene position: 3752769-3754715 (Clockwise)

Preceding gene: 218550938

Following gene: 218550940

Centisome position: 81.78

GC content: 49.26

Gene sequence:

>1947_bases
ATGATTGAAATCAAAAAACAAAATATAACGAGAAAAATCATCTCTCAACATTTGCTTGGCGGGAGAATGGTTGCCGGGGA
AGAGATTAGTATCGAGATTGATCACACCTTAACTCATGATGTGACAGGTACACCTGCTTATCTGGCTTTCGAAACATTAG
GGATTGCACAAGTAAAAACTAAACGGTCGGTCAGTTATATCGACCACAATATGCTGTATACCGATAATAAAAATCCAGAT
GACCATATTTATTTACAGACGGTGGCAAAAAAGCACGGGATTTATTTATCTAAAGCAGGCAACGGCATTTGCCACACAGT
ACATTTTGAGCGTTTCAGTCGTCCGGGGATCACGTTGTTAGGTTCAGACAGCCATACGCCAACGGGTGGAGCTGTTGGTG
CGCTGGCACTGGGTGCCGGTGGTCTGGATGTCGCGCTGGCGATGGCAGGAGAACCAACCTGGTTAAAAATGCCACAGGTT
ATTAATGTGCGCCTGCATGGTCGTATGCAACCTGGTGTTTCGGCGAAGGACGTCATACTGGAAATGTTACGCCGTGAAAC
CGTGAAAGGCGGTCTTGGTAAAGTGTATGAATATACTGGCGATGGTGTTGCACATATTAGTATCCGTGACCGCTCAACTA
TCACCAATATGGGCGCAGAAATGGGTGCTACCACGTCCATTTTCCCCAGTGACGAAATCACCCGTGAATTCTTTATTAAA
GAAAATCGCGAACATGAGTGGATTGCTCTGCAACCCGATGATGGCTGCGAATATGACGGTTATATCGATATCTACCTCGA
TAAGCTCGAACCTCTGGTCGCCATGCCGGATATGCCAGACAACGTGACCGAAGCGCGTAATTGCCGCGATGTGAAGATTA
ACCAGGTTTTCCTCGGCAGTTGTACGAACGCGTCATATACCGATTTCGCCAAAGCTGCGGCAGTACTTGAGGGGCGATTT
GCCCATCCGGAAGTGAGCTTTGTTGTCGCACCAGGCAGTCGCCAGTTGTATATGATGCTGGTGCGCGACGGCATTATCGG
CAAACTGATCGCTGCTGGCGCACGCGTAATTGACTGTGGTTGCGGCGCTTGCTGTGGCATCGGTCAGGCCCCTTCCACTG
ACGGTGTTTCTCTGCGCACCTCTAACCGCAACTTTAAAGGTCGCGGCGGGCATCTGAATGCCAAACTTTATCTTTCCAGC
CCGGAAGTAGCTGCGGCATCAGCGGTGACAGGTTATATCACTGACCCACGTGATCTGGCTGATATGTTGCTATTGCAGGA
TATCGTTGAACCGCAGGACTACATCATTGACGACCGACTGATTCTTGCTCCAGCGGACAACGGTGAAGAGATAGAAGTCA
TTCGTGGGCCGAATATCAAACCGATGCCGATCAACGATCCACTGCCAGACTCGTTGACTATCCATGTTTCAGCCGTGCGT
GGCGACAACATCACTACCGATGATATTACGCCTGCTAATGCGCATTTCTCCGCCCTGCGATCCAACATTCCAGAGATCAG
CAAATACTGCTACGGGCGTATTGACCCGGAATTTCACGACCGCTGCAAGCAACTGGGTATCAGCATCATTATTGGTGGCG
AAAACTATGGTCAGGGTTCCAGCCGTGAACATGCAGCTATCGCCCCTATGTATCTCGGCGTGAAGGCGGTTATTGCCAAA
TCGATGGCGCGTATCCACAAAAACAACCTGGTTAACCACGGTGTTGTGCCACTGATATTCGCCGACCCGGCAGATTACAC
CTGCTTCGATATGTTTGATGAATTACGTATCGAAAACTTCCATCAGGGGCTTCGTCAGCGGCGCGTATTGTTAAATAACG
TCACCAAAAACAGGCAGATCGCGTTATCTGTTGATCTCACCGATCGAGAAATTGAGATCGTGATCGACGGCGGCCAGTTA
CGCCATGTCGCAAACAAAAATGCGTAA

Upstream 100 bases:

>100_bases
ATTTGGCTGGGCTTTTGCCGCCATGATTCTTTGCTTTGTAATGGCGATTGTCGATATCTACAGCTTATTTTGCTAACAAT
TTTAAATACTGGAACAGACC

Downstream 100 bases:

>100_bases
TAACGGTTAGCGAAATGCAGGCGCTATGCCTGCATTAACGCCAGTTGAGGCATCCCTTATCTCTTTATCCAGGAATACTC
ATGCGAAAATTCAAAACAGT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MIEIKKQNITRKIISQHLLGGRMVAGEEISIEIDHTLTHDVTGTPAYLAFETLGIAQVKTKRSVSYIDHNMLYTDNKNPD
DHIYLQTVAKKHGIYLSKAGNGICHTVHFERFSRPGITLLGSDSHTPTGGAVGALALGAGGLDVALAMAGEPTWLKMPQV
INVRLHGRMQPGVSAKDVILEMLRRETVKGGLGKVYEYTGDGVAHISIRDRSTITNMGAEMGATTSIFPSDEITREFFIK
ENREHEWIALQPDDGCEYDGYIDIYLDKLEPLVAMPDMPDNVTEARNCRDVKINQVFLGSCTNASYTDFAKAAAVLEGRF
AHPEVSFVVAPGSRQLYMMLVRDGIIGKLIAAGARVIDCGCGACCGIGQAPSTDGVSLRTSNRNFKGRGGHLNAKLYLSS
PEVAAASAVTGYITDPRDLADMLLLQDIVEPQDYIIDDRLILAPADNGEEIEVIRGPNIKPMPINDPLPDSLTIHVSAVR
GDNITTDDITPANAHFSALRSNIPEISKYCYGRIDPEFHDRCKQLGISIIIGGENYGQGSSREHAAIAPMYLGVKAVIAK
SMARIHKNNLVNHGVVPLIFADPADYTCFDMFDELRIENFHQGLRQRRVLLNNVTKNRQIALSVDLTDREIEIVIDGGQL
RHVANKNA

Sequences:

>Translated_648_residues
MIEIKKQNITRKIISQHLLGGRMVAGEEISIEIDHTLTHDVTGTPAYLAFETLGIAQVKTKRSVSYIDHNMLYTDNKNPD
DHIYLQTVAKKHGIYLSKAGNGICHTVHFERFSRPGITLLGSDSHTPTGGAVGALALGAGGLDVALAMAGEPTWLKMPQV
INVRLHGRMQPGVSAKDVILEMLRRETVKGGLGKVYEYTGDGVAHISIRDRSTITNMGAEMGATTSIFPSDEITREFFIK
ENREHEWIALQPDDGCEYDGYIDIYLDKLEPLVAMPDMPDNVTEARNCRDVKINQVFLGSCTNASYTDFAKAAAVLEGRF
AHPEVSFVVAPGSRQLYMMLVRDGIIGKLIAAGARVIDCGCGACCGIGQAPSTDGVSLRTSNRNFKGRGGHLNAKLYLSS
PEVAAASAVTGYITDPRDLADMLLLQDIVEPQDYIIDDRLILAPADNGEEIEVIRGPNIKPMPINDPLPDSLTIHVSAVR
GDNITTDDITPANAHFSALRSNIPEISKYCYGRIDPEFHDRCKQLGISIIIGGENYGQGSSREHAAIAPMYLGVKAVIAK
SMARIHKNNLVNHGVVPLIFADPADYTCFDMFDELRIENFHQGLRQRRVLLNNVTKNRQIALSVDLTDREIEIVIDGGQL
RHVANKNA
>Mature_648_residues
MIEIKKQNITRKIISQHLLGGRMVAGEEISIEIDHTLTHDVTGTPAYLAFETLGIAQVKTKRSVSYIDHNMLYTDNKNPD
DHIYLQTVAKKHGIYLSKAGNGICHTVHFERFSRPGITLLGSDSHTPTGGAVGALALGAGGLDVALAMAGEPTWLKMPQV
INVRLHGRMQPGVSAKDVILEMLRRETVKGGLGKVYEYTGDGVAHISIRDRSTITNMGAEMGATTSIFPSDEITREFFIK
ENREHEWIALQPDDGCEYDGYIDIYLDKLEPLVAMPDMPDNVTEARNCRDVKINQVFLGSCTNASYTDFAKAAAVLEGRF
AHPEVSFVVAPGSRQLYMMLVRDGIIGKLIAAGARVIDCGCGACCGIGQAPSTDGVSLRTSNRNFKGRGGHLNAKLYLSS
PEVAAASAVTGYITDPRDLADMLLLQDIVEPQDYIIDDRLILAPADNGEEIEVIRGPNIKPMPINDPLPDSLTIHVSAVR
GDNITTDDITPANAHFSALRSNIPEISKYCYGRIDPEFHDRCKQLGISIIIGGENYGQGSSREHAAIAPMYLGVKAVIAK
SMARIHKNNLVNHGVVPLIFADPADYTCFDMFDELRIENFHQGLRQRRVLLNNVTKNRQIALSVDLTDREIEIVIDGGQL
RHVANKNA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=689, Percent_Identity=32.656023222061, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI8659555, Length=845, Percent_Identity=24.8520710059172, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI41352693, Length=725, Percent_Identity=25.6551724137931, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI87081781, Length=677, Percent_Identity=26.4401772525849, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786259, Length=443, Percent_Identity=29.1196388261851, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1787531, Length=776, Percent_Identity=24.6134020618557, Blast_Score=149, Evalue=8e-37,
Organism=Escherichia coli, GI2367097, Length=352, Percent_Identity=27.8409090909091, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=691, Percent_Identity=31.548480463097, Blast_Score=261, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI32564738, Length=686, Percent_Identity=31.6326530612245, Blast_Score=261, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25149342, Length=572, Percent_Identity=31.8181818181818, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17568399, Length=651, Percent_Identity=26.8817204301075, Blast_Score=151, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=696, Percent_Identity=31.0344827586207, Blast_Score=277, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6322261, Length=421, Percent_Identity=33.4916864608076, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6320440, Length=613, Percent_Identity=30.6688417618271, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321429, Length=640, Percent_Identity=27.34375, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI281365315, Length=716, Percent_Identity=31.0055865921788, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17864292, Length=716, Percent_Identity=31.0055865921788, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI161076999, Length=682, Percent_Identity=31.6715542521994, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI28571643, Length=631, Percent_Identity=32.8050713153724, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI17137564, Length=501, Percent_Identity=25.3493013972056, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645686, Length=261, Percent_Identity=27.9693486590038, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 70933; Mature: 70933

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKKQNITRKIISQHLLGGRMVAGEEISIEIDHTLTHDVTGTPAYLAFETLGIAQVKT
CEEEEHHHHHHHHHHHHHCCCEEEECCEEEEEECCEEECCCCCCCCEEEEECCCCEEHHH
KRSVSYIDHNMLYTDNKNPDDHIYLQTVAKKHGIYLSKAGNGICHTVHFERFSRPGITLL
CCCHHHHCCCEEEECCCCCCCEEEEEEEHHHCCEEEECCCCCEEEEEEHHHCCCCCEEEE
GSDSHTPTGGAVGALALGAGGLDVALAMAGEPTWLKMPQVINVRLHGRMQPGVSAKDVIL
ECCCCCCCCCCHHHHEECCCCCEEEEEECCCCCEEECCEEEEEEEECCCCCCCCHHHHHH
EMLRRETVKGGLGKVYEYTGDGVAHISIRDRSTITNMGAEMGATTSIFPSDEITREFFIK
HHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHCCHHCCCCEEECCCHHHHHHHHHC
ENREHEWIALQPDDGCEYDGYIDIYLDKLEPLVAMPDMPDNVTEARNCRDVKINQVFLGS
CCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCHHHHCCCCCEEEEEEEEEC
CTNASYTDFAKAAAVLEGRFAHPEVSFVVAPGSRQLYMMLVRDGIIGKLIAAGARVIDCG
CCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHCCCEEEECC
CGACCGIGQAPSTDGVSLRTSNRNFKGRGGHLNAKLYLSSPEVAAASAVTGYITDPRDLA
CCHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHHEEEECCCHHHHH
DMLLLQDIVEPQDYIIDDRLILAPADNGEEIEVIRGPNIKPMPINDPLPDSLTIHVSAVR
HHHHHHHHCCCCCEEECCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
GDNITTDDITPANAHFSALRSNIPEISKYCYGRIDPEFHDRCKQLGISIIIGGENYGQGS
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCC
SREHAAIAPMYLGVKAVIAKSMARIHKNNLVNHGVVPLIFADPADYTCFDMFDELRIENF
CCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
HQGLRQRRVLLNNVTKNRQIALSVDLTDREIEIVIDGGQLRHVANKNA
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCEEECCCCCC
>Mature Secondary Structure
MIEIKKQNITRKIISQHLLGGRMVAGEEISIEIDHTLTHDVTGTPAYLAFETLGIAQVKT
CEEEEHHHHHHHHHHHHHCCCEEEECCEEEEEECCEEECCCCCCCCEEEEECCCCEEHHH
KRSVSYIDHNMLYTDNKNPDDHIYLQTVAKKHGIYLSKAGNGICHTVHFERFSRPGITLL
CCCHHHHCCCEEEECCCCCCCEEEEEEEHHHCCEEEECCCCCEEEEEEHHHCCCCCEEEE
GSDSHTPTGGAVGALALGAGGLDVALAMAGEPTWLKMPQVINVRLHGRMQPGVSAKDVIL
ECCCCCCCCCCHHHHEECCCCCEEEEEECCCCCEEECCEEEEEEEECCCCCCCCHHHHHH
EMLRRETVKGGLGKVYEYTGDGVAHISIRDRSTITNMGAEMGATTSIFPSDEITREFFIK
HHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHCCHHCCCCEEECCCHHHHHHHHHC
ENREHEWIALQPDDGCEYDGYIDIYLDKLEPLVAMPDMPDNVTEARNCRDVKINQVFLGS
CCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCHHHHCCCCCEEEEEEEEEC
CTNASYTDFAKAAAVLEGRFAHPEVSFVVAPGSRQLYMMLVRDGIIGKLIAAGARVIDCG
CCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHCCCEEEECC
CGACCGIGQAPSTDGVSLRTSNRNFKGRGGHLNAKLYLSSPEVAAASAVTGYITDPRDLA
CCHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHHEEEECCCHHHHH
DMLLLQDIVEPQDYIIDDRLILAPADNGEEIEVIRGPNIKPMPINDPLPDSLTIHVSAVR
HHHHHHHHCCCCCEEECCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
GDNITTDDITPANAHFSALRSNIPEISKYCYGRIDPEFHDRCKQLGISIIIGGENYGQGS
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCC
SREHAAIAPMYLGVKAVIAKSMARIHKNNLVNHGVVPLIFADPADYTCFDMFDELRIENF
CCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
HQGLRQRRVLLNNVTKNRQIALSVDLTDREIEIVIDGGQLRHVANKNA
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]