Click here to switch to the map view.

The map label for this gene is yagI [H]

Identifier: 218550923

GI number: 218550923

Start: 3737685

End: 3738452

Strand: Direct

Name: yagI [H]

Synonym: EFER_3649

Alternate gene names: 218550923

Gene position: 3737685-3738452 (Clockwise)

Preceding gene: 218550914

Following gene: 218550924

Centisome position: 81.45

GC content: 53.91

Gene sequence:

>768_bases
ATGGCAGAAACCTCTTCAACCACCGCAGGCGCACAAACGTTATTACGCGGACTGGCGGTTCTTAATGCTGTTTATAACGG
CTGCCATGACCTGAAAAGTATCGGCGAGTTCACCGGCACGACCCGGAGCACAACCCATCGTTTAGTCACCGTTCTGGTGG
AACAACGCTACCTGCGCCATGTCCCAACGCAGGGCTACCAACTGGGTGCCAAACTGATCGAATTCGGTGCCAGAGCACTG
GAAAGCACCTCGCTGTACGAAGTTGCCCAGCCCGTTTTGCAACGCCTTGCCCGTTACACCCTGGACACCGTGCATCTGGG
GATCGTTGAAGGCGACGAAGTGCTGTATCTGGAGAAGATCAACAGCCAGCGCGGGCTGGAAATGCGCTCACGCCCAGGCC
ATCGCATGCCACTGGCAATCACCGGCATCGGCAAGGCATTGATCCTCAACCGAACAGAAGAAGAGTGGCGCACGCTGTTT
AAGACCTGTGGTGATGAAACAAAACTGGGCACATTTATTCAGAACATGCGTCGTTATGCCGCCAGTGGTTTTGCTTTTGA
TCTGGAAGAGAACGAGCCGACTATCCGTTGCGTTGCAGCGCCGGTATACAACGCCCGGGATGAAATTGTGGCGGCTATTT
CTGTCGCCAGTACCACTACGTATATGTCACTGGCGCGTCTGGAAGAACTGGCACCTTACGTCAAATCCTGTGCAGAAGAG
ATATCCGCTGAACTGGGCTGGGGTAAGCACGTCCACAAAAATAAATAA

Upstream 100 bases:

>100_bases
TTTATCCCACAATATGAGATGAAGTTTATATTGTGGGATATATCACCTTTTCCCCACTTCATTATGTGGAACACTTCAGC
AATATCATCAGGAGAAACGT

Downstream 100 bases:

>100_bases
ACCATGACTAAAGGAAACGGCTAATGTCGGGAAACACCTCATTTATTGCCCTGGACTGGGGAACGTCATCGCTTCGGGCA
TGGCGTTTTGGTGACTCACC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARAL
ESTSLYEVAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
KTCGDETKLGTFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVASTTTYMSLARLEELAPYVKSCAEE
ISAELGWGKHVHKNK

Sequences:

>Translated_255_residues
MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARAL
ESTSLYEVAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
KTCGDETKLGTFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVASTTTYMSLARLEELAPYVKSCAEE
ISAELGWGKHVHKNK
>Mature_254_residues
AETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALE
STSLYEVAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLFK
TCGDETKLGTFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVASTTTYMSLARLEELAPYVKSCAEEI
SAELGWGKHVHKNK

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=249, Percent_Identity=32.1285140562249, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788131, Length=218, Percent_Identity=32.1100917431193, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788581, Length=255, Percent_Identity=30.5882352941176, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=29.1187739463602, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082359, Length=255, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=26.2948207171315, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790752, Length=214, Percent_Identity=28.5046728971963, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28293; Mature: 28161

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTVLVEQRYLRH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VPTQGYQLGAKLIEFGARALESTSLYEVAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHH
NSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLFKTCGDETKLGTFIQNMRRYA
CCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVASTTTYMSLARLEELAPYVKSCAEE
HCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAELGWGKHVHKNK
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
AETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTVLVEQRYLRH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VPTQGYQLGAKLIEFGARALESTSLYEVAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHH
NSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLFKTCGDETKLGTFIQNMRRYA
CCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVASTTTYMSLARLEELAPYVKSCAEE
HCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAELGWGKHVHKNK
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]