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The map label for this gene is yiaO [H]

Identifier: 218550861

GI number: 218550861

Start: 3661396

End: 3662382

Strand: Direct

Name: yiaO [H]

Synonym: EFER_3577

Alternate gene names: 218550861

Gene position: 3661396-3662382 (Clockwise)

Preceding gene: 218550860

Following gene: 218550862

Centisome position: 79.79

GC content: 49.44

Gene sequence:

>987_bases
ATGAAATTACGTTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGTACATCTTCACTGGCGACACAATC
ATTACGTTTCGGTTATGAAACATCGCAAACCGACTCTCAACATATTGCGGCGAAAAAATTTAATGATTTATTGCAGGAGA
GAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGTACTCTCGGTAACGCACAAGCGATGATCAGTGGCGTGCGT
GGCGGCACTATTGATATGGAGATGTCTGGCTCGAATAACTTTGCCGGATTATCACCTGTGATGAACCTGCTTGATGTTCC
TTTCCTGTTCCGCGATACCGCCCACGCGCATAAAACGCTCGACGGCAAAGTCGGCGATGAACTGAAGGCCTCCCTTGAAG
GTAAGGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCACGCCAACCGGTTAAAACGCCT
GGCGACCTGAAAGGGCTGAAAATCCGCACCAACAACAGCCCGATGAATATCGCCGCATTTAAAGTATTTGGCGCTAACCC
TATCCCGATGCCGTTTGCCGAAGTCTATACCGGACTGGAAACACGCACTATCGACGCTCAGGAACATCCCATCAACGTCG
TCTGGTCGGCAAAATTTTATGAAGTGCAGAAGTACCTATCTCTGACTCACCATGCCTATTCCCCGCTTCTGGTAGTGATC
AACAAAGCGAAATTTGATGGCTTAAGCCCGGAGTTCCAGCAAGTGCTTATCTCATCAGCACAAGAAGCAGGTAACTACCA
GCGCAAACTGGTCGCCGAAAATCAGCAAAAAATCATCGACGGCATGAAAGACGCTGGCGTGGAAGTCATCACCGAACTTG
ATCGCAAAGCCTTTAGCGACGCTCTGGGTACTCAGGTCCGCGACATGTTTGTAAAAGATGTTCCGCAGGGCGCTGACCTG
CTGAAAGCCGTGGATGAGGTGCAATAA

Upstream 100 bases:

>100_bases
CCCTTACGTCCTGGTTTTATATTCCTTATTAGCCTTATTTGTTTTTATTCCCGATCTCATCATCCTCCCGTTGAAATGGA
TTAATTAAAAGGAAAATATT

Downstream 100 bases:

>100_bases
GCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGCCTGTATGACCGCGAAGGCCGGGA
GGCAGGCGTGCAGCGCCTGC

Product: transporter

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKLRSVTYALFIAGLAAFSTSSLATQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDELKASLEGKGLKVLAYWENGWRDVTNSRQPVKTP
GDLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQVLISSAQEAGNYQRKLVAENQQKIIDGMKDAGVEVITELDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ

Sequences:

>Translated_328_residues
MKLRSVTYALFIAGLAAFSTSSLATQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDELKASLEGKGLKVLAYWENGWRDVTNSRQPVKTP
GDLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQVLISSAQEAGNYQRKLVAENQQKIIDGMKDAGVEVITELDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ
>Mature_328_residues
MKLRSVTYALFIAGLAAFSTSSLATQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDELKASLEGKGLKVLAYWENGWRDVTNSRQPVKTP
GDLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQVLISSAQEAGNYQRKLVAENQQKIIDGMKDAGVEVITELDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=328, Percent_Identity=96.3414634146341, Blast_Score=656, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36118; Mature: 36118

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRSVTYALFIAGLAAFSTSSLATQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHHHHHHC
DGKVGDELKASLEGKGLKVLAYWENGWRDVTNSRQPVKTPGDLKGLKIRTNNSPMNIAAF
CCCCCHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLSPEFQQVLISSAQEAGNYQRKLVAENQQKIIDGMKDAGVEVITELDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGTQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLRSVTYALFIAGLAAFSTSSLATQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHHHHHHC
DGKVGDELKASLEGKGLKVLAYWENGWRDVTNSRQPVKTPGDLKGLKIRTNNSPMNIAAF
CCCCCHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLSPEFQQVLISSAQEAGNYQRKLVAENQQKIIDGMKDAGVEVITELDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGTQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]