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The map label for this gene is yiaK [H]

Identifier: 218550857

GI number: 218550857

Start: 3658035

End: 3659033

Strand: Direct

Name: yiaK [H]

Synonym: EFER_3573

Alternate gene names: 218550857

Gene position: 3658035-3659033 (Clockwise)

Preceding gene: 218550854

Following gene: 218550858

Centisome position: 79.72

GC content: 52.85

Gene sequence:

>999_bases
ATGAAAGTGTCATTTGAACAGTTAAAAGCAGCCTTCAATCGGGTATTACTTTCCCGGGGAGTGGCTGTTGAAACCGCCGA
TGCCTGTGCTGAAATGTTTGCCCGCACCACCGAATCAGGCGTTTATTCTCACGGTGTTAACCGCTTCCCACGCTTCATCC
AACAACTGGATAATGGCGATATCATTCCCGATGCCGAGGCCAAACGTATAACCACTCTTGGCGCTATCGAGCAATGGGAT
GCTCAGCGTTCAATCGGCAATCTGACGGCGAAAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGG
TCTGGTGGCGTTGCGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCTATATTG
GCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGCATCGGCACTAACCCGCTGATC
GTCGCCATTCCTTCTACCCCAATCACCATGGTCGATATGTCGATGTCGATGTTTTCCTACGGCATGTTAGAAGTTAACCG
CCTTGCAGGTCGTCAGCTCCCGGTCGATGGCGGCTTTGACGATGAAGGCAACCTCACCAAAGAACCTGGTGTAATCGAGA
AAAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGTATGTCGATCGTGCTGGATATGATCGCTACTCTCCTT
TCCGACGGCGCATCCGTTGCCGAAGTCACCCAGGATAACAGCGACGAATATGGCGTTTCGCAAATCTTTATCGCCATCGA
AGTGGATAAATTGATCGACGGCCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTACCGCTGAACGTG
CCGATGAAAACCAGGCCATTCGCTTACCCGGCCACGAATTTACCACCCTGCTGGCAGAAAACCGCCGTAACGGTATTACC
GTTGATGACAGCGTATGGGCTAAAATCCAGGCGTTATAA

Upstream 100 bases:

>100_bases
GATTGCTTTGTTAAATAGCGATCGATATATTTGAAATCAAATTTCGCATAGTGAAATTTAGCGATAAAAAAGCGATCACA
CAAAAATAAGGAATCCAGGG

Downstream 100 bases:

>100_bases
GGAGATAAGCCATGATTTTTGGACATATCGCGCAGCCTAACCCGTGCCGTTTTCCCGCCGCCATTGAAAAGGCGCTCGAT
TTTCTGCGCGCTACCGATTT

Product: 2,3-diketo-L-gulonate reductase

Products: NA

Alternate protein names: 2,3-DKG reductase; 3-dehydro-L-gulonate 2-dehydrogenase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKVSFEQLKAAFNRVLLSRGVAVETADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIPDAEAKRITTLGAIEQWD
AQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
SDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGIT
VDDSVWAKIQAL

Sequences:

>Translated_332_residues
MKVSFEQLKAAFNRVLLSRGVAVETADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIPDAEAKRITTLGAIEQWD
AQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
SDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGIT
VDDSVWAKIQAL
>Mature_332_residues
MKVSFEQLKAAFNRVLLSRGVAVETADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIPDAEAKRITTLGAIEQWD
AQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
SDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGIT
VDDSVWAKIQAL

Specific function: Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790000, Length=332, Percent_Identity=96.9879518072289, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1787020, Length=331, Percent_Identity=24.773413897281, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786727, Length=331, Percent_Identity=23.8670694864048, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17507179, Length=313, Percent_Identity=24.6006389776358, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536633, Length=261, Percent_Identity=24.5210727969349, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24667908, Length=293, Percent_Identity=25.2559726962457, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24667904, Length=291, Percent_Identity=25.4295532646048, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45553211, Length=293, Percent_Identity=25.2559726962457, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24667912, Length=293, Percent_Identity=25.2559726962457, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24658010, Length=291, Percent_Identity=24.3986254295533, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648360, Length=332, Percent_Identity=24.3975903614458, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.130 [H]

Molecular weight: Translated: 36502; Mature: 36502

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSFEQLKAAFNRVLLSRGVAVETADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGD
CCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
IIPDAEAKRITTLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCC
GSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPITMVDMSMSMFSY
CCCCCHHCCCCEEEEEEECCEEEECCCCCCCCEECCCCEEEEECCCCEEEEEHHHHHHHH
GMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
SDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAERADENQAI
HCCCCEEHHHCCCCCCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
RLPGHEFTTLLAENRRNGITVDDSVWAKIQAL
EECCHHHHHHHHHCCCCCCEECCHHHEEEECC
>Mature Secondary Structure
MKVSFEQLKAAFNRVLLSRGVAVETADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGD
CCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
IIPDAEAKRITTLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCC
GSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPITMVDMSMSMFSY
CCCCCHHCCCCEEEEEEECCEEEECCCCCCCCEECCCCEEEEECCCCEEEEEHHHHHHHH
GMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
SDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAERADENQAI
HCCCCEEHHHCCCCCCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
RLPGHEFTTLLAENRRNGITVDDSVWAKIQAL
EECCHHHHHHHHHCCCCCCEECCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA