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The map label for this gene is yhjW [H]

Identifier: 218550827

GI number: 218550827

Start: 3623827

End: 3625683

Strand: Reverse

Name: yhjW [H]

Synonym: EFER_3543

Alternate gene names: 218550827

Gene position: 3625683-3623827 (Counterclockwise)

Preceding gene: 218550828

Following gene: 218550826

Centisome position: 79.01

GC content: 48.52

Gene sequence:

>1857_bases
TTGTGCTTCAGACACACGCTTTCCAGACACTTTTTCTTTAACCTGTGGTCTACTAGCCTGCGCTGTGGATTTCATTACTA
TCGGCAACCTCCGCGTATTTCACCTGCCAGGTCACATAGATCATCACGCAGGGATTTTTCACCTGTTTTTCCAGGGTTTG
TTTGCATGAGATATATCAAAACGATAACGCAGCAAAAGCTAAGCTTTTTGCTCGCTGTCTATATCGGTCTGTTTATGAAT
TGCGCAGTCTATTTCCGTCGGTTTGACGGTTACGCGCATAACTTCACTTTTCTTAATGGCGTCTCGGCGGTTATCGAACT
GGCAGGGACGGTTCTGGCAACCTTCTTCTTTTTACGTTTAATTTCACTCTTTGGCAGAACGGCCTGGAAAGTGCTGGCTT
CATTGCTGGTGCTGATTTCTGCTGGAGCCAGCTACTATATGACATTTATGAATGTCATGATTGGCTACGGGATTGTTGCT
TCGGTGATGACCACCGACATTGACCTGTCGAAAGAAGTGGTTGGCTGGACGCTTATCGCATGGGTTGTACTGGTGAGTAT
TATCCCGTTGCTATTTATCTGGATGAACCGCTCACAAGACACCTTCTGGCGTCAGCTGTGTACCCCGAAAACACGCTGGC
GTAGTGCGCTTACTGTACTGCTGGCGGGTTTACTGGTATGGGGCCCAATCCGTCTTATGGATATAGCACAAAAGCGTGCA
GACCGCATGGCTGGGGTGGATATGCCAAGTTATGGCGGGGTGCTGGCGAACTCTTATTTACCCTCGAACTGGCTTTCTGC
GCTGGGTCTGTACGCTTGGGCGCAAGTGGATGAATCGTCGGACAATCGGTCGCTGATGAATCCGGCAAAAAAATTCACCT
ATACTCCGACACAAAATCTCGACGATACCTATGTGGTTTTCATTATTGGTGAAACCACGCGTTGGGATCATATGGGGATG
CTGGGCTACGAGCGTGATACCACGCCGAAGCTGGCGAAAGAGAAGAACCTGGTGATGTACCGTGGTTATTCTTGCGATAC
TGCGACTAAATTATCACTACGCTGTATGTTTGTGCGTGAAGGTGGAACGGAAGATAACCCACAACGTACGCTTAAAGAGC
AGAACGTATTTGCGGTTCTCAAACAGTTGGGCTTTAGCTCTGATTTGTACGCCATGCAGAGTGAGATGTGGTTCTACAGC
AACACCATGGCTGACAACATTGCCTATCGTGAGCAAATCGCTGCTGAGCCACGTAACCGTGGGAAAGCGGTGGACGACAT
GTTGCTGGTCAATGAAATCGAGCGTTCTCTGGAGAAAAAGCCAGAAGGTAAGCATCTGATTGTCTTACATACCAAAGGGT
CGCACTACAACTATACGCAGCGTTATCCGCGTGAGTTTGCCCAGTGGCAGCCAGAGTGCTATGCAATTGATCGCAAATGC
CCACGAGCGCCGATGATTAACTCATACGATAACTCGATCACCTACGTTGATCATTTTATCGATACAGTGATCGACAAATT
CCGCGATAAGAAGGCCATTATTTTCTATGCAGCGGACCACGGTGAGTCAATTAACGAGCGTGAGCACTTGCATGGTACAC
CACGTGAATATGCGCCACCGGAGCAGTTCCGCGTACCGATGATGGTCTGGATGTCGGATAAATATCTGGAGAATCCGGCC
AACGCGCAGGCATTTGCGCAACTGAAAAAAGAGGCGGATATCAAAGTGCCACGCCGTCATGTGGAACTGTACGACACCAT
TTTAGGTTGCCTTGGTTATACTTCACCGGACGGTGGTATCAACGAAAACAACAACTGGTGTCGAATCCCTCAGGCGAAGA
AAGCAGAAGCTAACTAA

Upstream 100 bases:

>100_bases
CACTTGAATATTTACCATCAATTGCAAGTCAGGTGACCCCCACATTTTTAACGGCCTCACTAAACCATAAGTCTCAATTC
ATACTTGCGCTTTTGTAAAT

Downstream 100 bases:

>100_bases
ATGCACTGCTGAGTTTCTCGCCGATCAAAAGGCATTTTGCTATTAAGGGATTGACGAGGGCGTATCTGCGCAGTAAGATG
CGCCCCGCATTCGGTGATTG

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MCFRHTLSRHFFFNLWSTSLRCGFHYYRQPPRISPARSHRSSRRDFSPVFPGFVCMRYIKTITQQKLSFLLAVYIGLFMN
CAVYFRRFDGYAHNFTFLNGVSAVIELAGTVLATFFFLRLISLFGRTAWKVLASLLVLISAGASYYMTFMNVMIGYGIVA
SVMTTDIDLSKEVVGWTLIAWVVLVSIIPLLFIWMNRSQDTFWRQLCTPKTRWRSALTVLLAGLLVWGPIRLMDIAQKRA
DRMAGVDMPSYGGVLANSYLPSNWLSALGLYAWAQVDESSDNRSLMNPAKKFTYTPTQNLDDTYVVFIIGETTRWDHMGM
LGYERDTTPKLAKEKNLVMYRGYSCDTATKLSLRCMFVREGGTEDNPQRTLKEQNVFAVLKQLGFSSDLYAMQSEMWFYS
NTMADNIAYREQIAAEPRNRGKAVDDMLLVNEIERSLEKKPEGKHLIVLHTKGSHYNYTQRYPREFAQWQPECYAIDRKC
PRAPMINSYDNSITYVDHFIDTVIDKFRDKKAIIFYAADHGESINEREHLHGTPREYAPPEQFRVPMMVWMSDKYLENPA
NAQAFAQLKKEADIKVPRRHVELYDTILGCLGYTSPDGGINENNNWCRIPQAKKAEAN

Sequences:

>Translated_618_residues
MCFRHTLSRHFFFNLWSTSLRCGFHYYRQPPRISPARSHRSSRRDFSPVFPGFVCMRYIKTITQQKLSFLLAVYIGLFMN
CAVYFRRFDGYAHNFTFLNGVSAVIELAGTVLATFFFLRLISLFGRTAWKVLASLLVLISAGASYYMTFMNVMIGYGIVA
SVMTTDIDLSKEVVGWTLIAWVVLVSIIPLLFIWMNRSQDTFWRQLCTPKTRWRSALTVLLAGLLVWGPIRLMDIAQKRA
DRMAGVDMPSYGGVLANSYLPSNWLSALGLYAWAQVDESSDNRSLMNPAKKFTYTPTQNLDDTYVVFIIGETTRWDHMGM
LGYERDTTPKLAKEKNLVMYRGYSCDTATKLSLRCMFVREGGTEDNPQRTLKEQNVFAVLKQLGFSSDLYAMQSEMWFYS
NTMADNIAYREQIAAEPRNRGKAVDDMLLVNEIERSLEKKPEGKHLIVLHTKGSHYNYTQRYPREFAQWQPECYAIDRKC
PRAPMINSYDNSITYVDHFIDTVIDKFRDKKAIIFYAADHGESINEREHLHGTPREYAPPEQFRVPMMVWMSDKYLENPA
NAQAFAQLKKEADIKVPRRHVELYDTILGCLGYTSPDGGINENNNWCRIPQAKKAEAN
>Mature_618_residues
MCFRHTLSRHFFFNLWSTSLRCGFHYYRQPPRISPARSHRSSRRDFSPVFPGFVCMRYIKTITQQKLSFLLAVYIGLFMN
CAVYFRRFDGYAHNFTFLNGVSAVIELAGTVLATFFFLRLISLFGRTAWKVLASLLVLISAGASYYMTFMNVMIGYGIVA
SVMTTDIDLSKEVVGWTLIAWVVLVSIIPLLFIWMNRSQDTFWRQLCTPKTRWRSALTVLLAGLLVWGPIRLMDIAQKRA
DRMAGVDMPSYGGVLANSYLPSNWLSALGLYAWAQVDESSDNRSLMNPAKKFTYTPTQNLDDTYVVFIIGETTRWDHMGM
LGYERDTTPKLAKEKNLVMYRGYSCDTATKLSLRCMFVREGGTEDNPQRTLKEQNVFAVLKQLGFSSDLYAMQSEMWFYS
NTMADNIAYREQIAAEPRNRGKAVDDMLLVNEIERSLEKKPEGKHLIVLHTKGSHYNYTQRYPREFAQWQPECYAIDRKC
PRAPMINSYDNSITYVDHFIDTVIDKFRDKKAIIFYAADHGESINEREHLHGTPREYAPPEQFRVPMMVWMSDKYLENPA
NAQAFAQLKKEADIKVPRRHVELYDTILGCLGYTSPDGGINENNNWCRIPQAKKAEAN

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=563, Percent_Identity=79.7513321492007, Blast_Score=947, Evalue=0.0,
Organism=Escherichia coli, GI87082372, Length=542, Percent_Identity=23.9852398523985, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI87082223, Length=402, Percent_Identity=26.865671641791, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790392, Length=507, Percent_Identity=21.301775147929, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 71249; Mature: 71249

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEECCCCCCCCCC
>Mature Secondary Structure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CCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]