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The map label for this gene is arsB [H]

Identifier: 218550787

GI number: 218550787

Start: 3574326

End: 3575615

Strand: Direct

Name: arsB [H]

Synonym: EFER_3498

Alternate gene names: 218550787

Gene position: 3574326-3575615 (Clockwise)

Preceding gene: 218550786

Following gene: 218550788

Centisome position: 77.89

GC content: 54.5

Gene sequence:

>1290_bases
ATGTTACTGGCGGGCGCTATCTTTGTCCTGACCATCGTATTGGTTATCTGGCAGCCGAAAGGTTTAGGCATCGGCTGGAG
TGCGACACTGGGCGCCGTACTGGCGTTAGTTACTGGCGTGGTCCATCCGGGCGATATTCCGGTGGTGTGGAATATCGTCT
GGAACGCGACGGCTGCGTTTATTGCCGTCATTATCATCAGCCTGCTGCTGGATGAGTCCGGCTTTTTTGAATGGGCGGCG
CTGCACGTCTCACGCTGGGGCAATGGGCGCGGTCGCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTACCGTTGCGGC
CCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACGCCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGCAAAG
GCACTACGCTGGCGTTTGTTATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAATCTGGTG
AATATCGTTTCAGCAGATTTCTTTGACCTCGGCTTTCGCGAATATGCCTCGGTGATGGTGCCGGTGGATATCGCCGCGAT
TGTTGCCACGCTGGTGATGCTGCATCTCTTTTTTCGCAAAGATATTCCGCAGAACTACGACATGGCGCTGCTAAAATCTC
CCGCAGATGCGATTAAAGATCCTGCGACGTTCAAAACTGGCTGGGTTGTTTTACTGCTTCTGCTGGTGGGATTTTTCGTC
CTGGAACCGCTCGGCATTCCGGTAAGCGCTATTGCGGCAGTAGGCGCGCTGATATTATTTGCCGTCGCTAAACGCGGTCA
TGCGATTAATACGGGTAAAGTGCTGCGCGGTGCCCCCTGGCAGATTGTCATCTTCTCGCTCGGCATGTATCTGGTGGTTT
ACGGCCTGCGCAATGCCGGACTAACGGAATATCTTTCTGACGTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCG
ACGCTCGGTACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTC
CATTGATGGCAGCACGGCGTCTGGTGTCATCAAAGAGGCGATGGTTTATGCCAACGTGATTGGCTGCGATTTGGGGCCGA
AAATTACACCGATTGGTAGCCTGGCAACGCTGCTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTATCAGCTGGGGA
TATTACTTCCGTACAGGGATTATCATGACCCTACCGGTGCTGTTTGTGACGCTCGCCGCGCTGGCGCTTCGTCTCTCTTT
CACATCGTAA

Upstream 100 bases:

>100_bases
CGTCAGACAAAATTGTTCCGCAGACAGTAAGAACATTTGTAGTTAAAAAATTTATCTAAACACATATGAATATTCGAATG
TGTTTTATCCAGGAGGCATG

Downstream 100 bases:

>100_bases
TGAGATACTGATATGAGCAACATTACTATTTATCACAACCCGGCCTGCGGCACGTCGCGTAATACGCTGGAGATGATTCG
CAACAGCGGTACAGAACCGA

Product: arsenite/antimonite transporter

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGATVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFDLGFREYASVMVPVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPADAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTS

Sequences:

>Translated_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGATVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFDLGFREYASVMVPVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPADAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTS
>Mature_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGATVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFDLGFREYASVMVPVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPADAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTS

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=428, Percent_Identity=98.5981308411215, Blast_Score=818, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45560; Mature: 45560

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGATVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFDLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
PVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPADAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSDVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTS
HHHHHHCCC
>Mature Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGATVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFDLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
PVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPADAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSDVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]