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The map label for this gene is rng [H]

Identifier: 218550530

GI number: 218550530

Start: 3311420

End: 3312889

Strand: Reverse

Name: rng [H]

Synonym: EFER_3224

Alternate gene names: 218550530

Gene position: 3312889-3311420 (Counterclockwise)

Preceding gene: 218550531

Following gene: 218550529

Centisome position: 72.2

GC content: 49.86

Gene sequence:

>1470_bases
ATGACGGCTGAATTGTTGGTAAACGTAACGCCATCGGAAACACGCGTGGCGTATATTGATGGCGGTATTCTGCAGGAAAT
TCATATTGAACGTGAGGCGCGACGCGGAATCGTAGGCAATATCTACAAAGGTCGTGTGAGTCGTGTACTTCCGGGTATGC
AGGCGGCTTTTGTAGATATTGGCCTGGAAAAAGCCGCATTCCTCCATGCTTCCGATATTATGCCGCATACCGAATGTGTG
GCAGGTGATGAACAAAAGCAGTTTACCGTCCGGGATATCTCTGAACTGGTGCGTCAGGGGCAGGATCTGATGGTGCAGGT
GGTCAAAGATCCCCTTGGCACTAAAGGGGCGCGCCTGACCACTGATATCACTTTGCCTTCTCGTTATCTGGTATTTATGC
CAGGAGCTTCACATGTTGGAGTTTCTCAACGCATTGAAAGTGAGTCTGAACGCGAACGCCTGAAAAAGGTGGTGGCAGAC
TATTGTGATGAGCAGGGCGGATTTATTATCCGAACAGCGGCAGAAGGCGTAGGAGAGGCGGAACTGGCTTCTGACGCGGC
TTATCTCAAGCGCGTGTGGACCAAAGTGATGGAGCGCAAAAAGCGCCCACAAACCCGCTATCAGCTTTATGGTGAACTGG
CCCTGGCACAGCGTGTATTAAGAGATTTTGCTGATGCTGAGCTGGATCGGATCCGCGTGGACTCTCGTTTAACCTATGAA
GCATTGCTGGAATTTACCGCTGAGTACATTCCCGAAATGACCAGCAAACTGGAACATTACAGCGGGCGCCAGCCGATTTT
CGATCTCTTTGATGTGGAAAATGAGATCCAGCGGGCGCTGGAGCGTAAAGTTGAGTTGAAATCTGGCGGCTACCTGATCA
TTGATCAGACGGAAGCGATGACCACAGTAGATATCAACACCGGCGCATTTGTCGGCCATCGCAACCTGGATGACACCATA
TTCAACACCAACATCGAAGCAACACAAGCGATTGCTCGCCAACTGCGTTTACGTAACCTTGGTGGCATAATCATTATTGA
TTTCATTGATATGAATAATGAAGATCACCGTCGTCGGGTACTCCATTCGCTGGAGCAGGCGCTGAGTAAAGATCGGGTAA
AAACCAGCGTTAACGGATTTTCAGCTCTGGGGCTGGTGGAGATGACACGCAAGCGCACCCGCGAAAGTATTGAGCATGTG
TTGTGTAACGAATGCCCAACGTGCCATGGCCGTGGAACGGTGAAAACGGTAGAAACCGTCTGCTATGAGATCATGCGTGA
AATCGTGCGCGTGCATCATGCTTATGACTCTGATCGCTTCCTCGTCTATGCTTCTCCGGCAGTGGCAGAAGCGCTGAAGG
GAGAAGAGTCACACGCGCTGGCAGAAGTGGAAATTTTTGTTGGTAAACAAGTTAAAGTACAAATCGAACCGCTCTATAAC
CAGGAGCAGTTTGACGTTGTAATGATGTAA

Upstream 100 bases:

>100_bases
AAGATAAATGGCAGCTATCACGCCGTAGTCGGCTTACCGCTGGTTGAAACGTATGAATTATTAAGTAATTTTAACGCACT
GCGTGAAGAAAGGGATAAAC

Downstream 100 bases:

>100_bases
ACCCGCACCGGGCCGCCAGTCAGACGGCCCTTTTTAGGCAGACAAGGAGAGACGCGTGAGGCGATTGCCGGGGATTTTAC
TGCTCACTGGAGCCACGCTC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLEKAAFLHASDIMPHTECV
AGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAD
YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
ALLEFTAEYIPEMTSKLEHYSGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM

Sequences:

>Translated_489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLEKAAFLHASDIMPHTECV
AGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAD
YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
ALLEFTAEYIPEMTSKLEHYSGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM
>Mature_488_residues
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLEKAAFLHASDIMPHTECVA
GDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVADY
CDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYEA
LLEFTAEYIPEMTSKLEHYSGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIF
NTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVL
CNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQIEPLYNQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=98.7730061349693, Blast_Score=996, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=436, Percent_Identity=35.7798165137615, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55305; Mature: 55174

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
GLEKAAFLHASDIMPHTECVAGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVADYCDEQGGFIIRTAAEGVGEA
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
ELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEECCCCHHHH
ALLEFTAEYIPEMTSKLEHYSGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCEEEEC
>Mature Secondary Structure 
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
GLEKAAFLHASDIMPHTECVAGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVADYCDEQGGFIIRTAAEGVGEA
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
ELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEECCCCHHHH
ALLEFTAEYIPEMTSKLEHYSGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]