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The map label for this gene is mcrB [H]

Identifier: 218550413

GI number: 218550413

Start: 3196929

End: 3199004

Strand: Reverse

Name: mcrB [H]

Synonym: EFER_3105

Alternate gene names: 218550413

Gene position: 3199004-3196929 (Counterclockwise)

Preceding gene: 218550414

Following gene: 218550412

Centisome position: 69.71

GC content: 45.62

Gene sequence:

>2076_bases
ATGACTCTTGTAGATAGTGTCGAAGCAGGTAGGCTAACGATTAGTGAATTGATTGATGCCCTCGCGAAAGATAAAAATTA
CACAGCTTCCAAATGGTATCAGCGATACCGTGCATTTACGACTTTGCTACAGCAAACCTCAACTTTTACTGAGCCTGCAA
CAGATGGTCTGGTCAAACAGCTTTGGTATGAGCGTGACAACGGCATTGCAAGTATTCGCCAGGGCGTTCCATCCTTAGCA
GAATATCAGCAAAGCCTGCCACTGCTTAGAGAACTAACTGAACGAATTCGGCAACAGCCGGATGAAGAAACTTACCAATA
TGTTGGCAATGCACTACAACAAGCTAAAGAAAACGGACTTCTCAAGCGTATGTATTGGAGTTTGAGAAATCGCGTCTTTG
CCGCGTTCTCGCCAGAAAACTACACCAGTACTGTGGATGAGAATGCTTTTAATAAAGCAGCAGAATTCCTAAATCAGCAC
TTCCATCTCGGTTTGGCACTAACCGGAAATTGGTTACAGAAAAACTATGAATTGAAACAAGCCATACACGCCCAATCTCC
TGACACAGATCCTTATTACGTGAATATGGCCATCTGGCATCTCTATGAATTGCTCCGTGAACGCGATAATGAACAAAAGC
AGGAGAAAATAGCGAGCACTACATTCATAACCAGCAGTGAGCCCATCGAGAACAAGATCATCCTACATTCACCAACTAAC
GTGATCTTCTTTGGCCCCCCTGGCACTGGCAAAACCTTCAGGTTGCAGCAAAAAATGAAAGAGTACACTTCTCATGCTGT
TCCAGCTGATCGTGATGCCTGGCTGGATTCTCGCCTTGAATCGTTGAATTGGATGCAGGTTATAACGCTGGTGCTGCTCG
ATCTTGGGAAACGAGCCAAAGTTCGCCAAATTATTGAACATATGTGGTTTCAACGTAAGGCATTATTAAACGGTCGTAAT
GGCAACCTATCGAATACAGCCTGGGCAGCTTTGCAATCCTATACAGTTCCCGAGTCGTTAACCGTTGATTATAAGAATCG
GCGTGAGCCTGCCGTATTTAACAAAACAGATAACAGCGAATGGTTGCTAGTTGATTCACAGCTCGAGCAAGTGGAGGATT
TGGTAGAGCTCTACGCCGAACTTAAACGTGGCCCTAAATCTGCCGAAGCCATCCAGCGTTTTGCGGTGGTTACGTTCCAC
CAATCTTACGGCTATGAAGAATTTATTGAAGGTATACGCGCTCGCTCTGATGAGAGTGGCAATATCTCTTATCCCATTGA
ACCGGGTATCTTTATGCGCCTTTGCCAACGTGCGAATGCCGATCCAGGACATCGCTACGCCATTTTTATTGATGAGATCA
ATCGCGGCAACATATCCAAGATCTTTGGTGAACTGATCTCACTCATTGAAGTTGACAAGCGTGCAGGCATGCCCAATGCA
ATGAGCCTGCAACTGGCTTATAGCGGTGATCACTTCAGCGTACCCGCCAATGTCGATATCATCGGAGCCATGAATACAGC
GGACCGTTCTTTAGCTCTGATGGACACGGCTTTGCGCCGTCGCTTTGACTTTGTCGAAATGATGCCCGATCTCTCTTTAC
TGAGTGGAGCTAAGGTGAAAGGCATAGAGCTCGAGTCGTTGTTAGAGAAACTCAATAGCCGCATCGAGGCTCTTTACGAT
CGTGAACATACGCTGGGGCATGCGTTCTTTATGCCGGTAAAAAATGCACTCGATGCCGATGATGAAGAAGCTGCGTTTAA
ACAATTGAAGATCGCATTCCAGAAAAAGATCATTCCGCTTTTACAGGAATACTTTTTCGATGACTGGAACAAGATCCGGT
TGGTGCTGGCAGACAATCAAAAGCAAGACGACAACCTGCAATTCGTGATTGAGAAAACCGACGATCTCGATACGCTTTTT
GGTAACAACCATGGTTTACGACGCCATGATCAGCAATCAACAGCTTACGAGCTCAAAGATTTCGATCAAGAGATCTGGAA
TATTCCACAGGCTTATCGTTCAATTTATCAGCCCCAGCAGACTCCCCTTGATGAGCAGGCTGTAAATCATGGGTGA

Upstream 100 bases:

>100_bases
TATATATAATCGGCACTAGTAACAACGATTAACTCAATATAGTTAGAATGCCTCATATTAATTAAAGAATAAGTAATGCC
ACAACAGAGGAAAAGTCACG

Downstream 100 bases:

>100_bases
AGTTATCTCCGTTTTTGAATATGACCTACTGGGGAGTGACAAAGCGGCTTCAATTGGCGCAAAACCGGTGCCTCCACTGG
TCTTTAATTATTTAGAAGCG

Product: 5-methylcytosine-specific restriction enzyme with GTPase activity

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTLVDSVEAGRLTISELIDALAKDKNYTASKWYQRYRAFTTLLQQTSTFTEPATDGLVKQLWYERDNGIASIRQGVPSLA
EYQQSLPLLRELTERIRQQPDEETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIASTTFITSSEPIENKIILHSPTN
VIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLDSRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRN
GNLSNTAWAALQSYTVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQRFAVVTFH
QSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNA
MSLQLAYSGDHFSVPANVDIIGAMNTADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYD
REHTLGHAFFMPVKNALDADDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNLQFVIEKTDDLDTLF
GNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTPLDEQAVNHG

Sequences:

>Translated_691_residues
MTLVDSVEAGRLTISELIDALAKDKNYTASKWYQRYRAFTTLLQQTSTFTEPATDGLVKQLWYERDNGIASIRQGVPSLA
EYQQSLPLLRELTERIRQQPDEETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIASTTFITSSEPIENKIILHSPTN
VIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLDSRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRN
GNLSNTAWAALQSYTVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQRFAVVTFH
QSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNA
MSLQLAYSGDHFSVPANVDIIGAMNTADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYD
REHTLGHAFFMPVKNALDADDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNLQFVIEKTDDLDTLF
GNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTPLDEQAVNHG
>Mature_690_residues
TLVDSVEAGRLTISELIDALAKDKNYTASKWYQRYRAFTTLLQQTSTFTEPATDGLVKQLWYERDNGIASIRQGVPSLAE
YQQSLPLLRELTERIRQQPDEETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQHF
HLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIASTTFITSSEPIENKIILHSPTNV
IFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLDSRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNG
NLSNTAWAALQSYTVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQRFAVVTFHQ
SYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAM
SLQLAYSGDHFSVPANVDIIGAMNTADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDR
EHTLGHAFFMPVKNALDADDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNLQFVIEKTDDLDTLFG
NNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTPLDEQAVNHG

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=236, Percent_Identity=39.8305084745763, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 79570; Mature: 79438

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVDSVEAGRLTISELIDALAKDKNYTASKWYQRYRAFTTLLQQTSTFTEPATDGLVKQ
CCCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDEETYQYVGNALQQAKENGL
HHHHCCCCHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
LKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQHFHLGLALTGNWLQKNYELKQ
HHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHH
AIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIASTTFITSSEPIENKIILHSPTN
HHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEECCCC
VIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLDSRLESLNWMQVITLVLLDLGKRAK
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSYTVPESLTVDYKNRREPAVFNKTDNSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEECCCCCC
WLLVDSQLEQVEDLVELYAELKRGPKSAEAIQRFAVVTFHQSYGYEEFIEGIRARSDESG
EEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
NISYPIEPGIFMRLCQRANADPGHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNA
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
MSLQLAYSGDHFSVPANVDIIGAMNTADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVK
EEEEEEECCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
GIELESLLEKLNSRIEALYDREHTLGHAFFMPVKNALDADDEEAAFKQLKIAFQKKIIPL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LQEYFFDDWNKIRLVLADNQKQDDNLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKD
HHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHH
FDQEIWNIPQAYRSIYQPQQTPLDEQAVNHG
HHHHHHCCHHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TLVDSVEAGRLTISELIDALAKDKNYTASKWYQRYRAFTTLLQQTSTFTEPATDGLVKQ
CCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDEETYQYVGNALQQAKENGL
HHHHCCCCHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
LKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQHFHLGLALTGNWLQKNYELKQ
HHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHH
AIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIASTTFITSSEPIENKIILHSPTN
HHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEECCCC
VIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLDSRLESLNWMQVITLVLLDLGKRAK
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSYTVPESLTVDYKNRREPAVFNKTDNSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEECCCCCC
WLLVDSQLEQVEDLVELYAELKRGPKSAEAIQRFAVVTFHQSYGYEEFIEGIRARSDESG
EEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
NISYPIEPGIFMRLCQRANADPGHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNA
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
MSLQLAYSGDHFSVPANVDIIGAMNTADRSLALMDTALRRRFDFVEMMPDLSLLSGAKVK
EEEEEEECCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
GIELESLLEKLNSRIEALYDREHTLGHAFFMPVKNALDADDEEAAFKQLKIAFQKKIIPL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LQEYFFDDWNKIRLVLADNQKQDDNLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKD
HHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHH
FDQEIWNIPQAYRSIYQPQQTPLDEQAVNHG
HHHHHHCCHHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]