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The map label for this gene is hsdS [H]

Identifier: 218550389

GI number: 218550389

Start: 3158047

End: 3159555

Strand: Direct

Name: hsdS [H]

Synonym: EFER_3081

Alternate gene names: 218550389

Gene position: 3158047-3159555 (Clockwise)

Preceding gene: 218550388

Following gene: 218550390

Centisome position: 68.82

GC content: 41.82

Gene sequence:

>1509_bases
ATGAGTGCGGGGAAATTGCCGGAGGGGTGGGTTGAAACTAATCTGCAAAACGTCGCATCATGGGGCTCAGGTGGAACACC
CTCCCGAAATCATGATGAGTATTACAATGGCAATATACCCTGGATTAAAACAGGTGATTTAGGGCCTAAAATTATTACTA
ATGCCAGTGAGTATATAACCGATGCTGGTGTACAAAACTCAAGTGCAAAATTCTTTCCTAAGGGTTCTGTCGCTATTGCA
ATGTATGGAGCAACTATTGGTAAAACATCCATATTAGGCATTGACGCAACCACAAACCAGGCATGTGCAGTGGGAACCCC
TCTTGAAGGCATCACATCCACCCTTTTTCTATATTATTTTTTACTTAATGAAAAAAATGCATTTATAAAAAAAGGAAAAG
GTGGTGCACAACCTAATATTTCTCAAACAGTGATAAAAGAGCACATAATTTACTTGCCACCACTTGCCGAGCAAAAAATC
ATCACTGAAAAACTCGATACGCTGCTGGCGCAGGTAGACAGCACCAAAGCACGTCTTGAGCAAATCCCACAAATCCTGAA
ACGTTTTCGTCAGGCAGTGCTGGAACGAGCTGTTAATGGGAAACTTACAGAATGTTGGAGAGATTGCGTAGGTGAACTAA
CCTCAGCAGAAGAAATCATTACTGAAATTAAAAAGTATAGAAAAGCATCACTTTCCACTGAAGGCTCATCCGCATCAACT
GAATCCAAGCGGCAAATAGCAAAAATTGAAAAACATTGCTTTAAAGTACCTAAAATAAATCTACCAAAAGGATGGGTTTG
GACAACATTTCTTCAGTCAATGGAAAAAGTTGTTGATTGCCATAACAAAACAGCCCCATATGTTGATCAGGGCATTCACT
TGATAAGAACTCCAGATATTCGCAATGGTGTTATATCATTAGATAATACAAAATATATTGACAATGACACATATCTTTAC
TGGTCTAAAAGATGTCCTCCGAGAAGTGGAGATATTATATTTACACGAGAAGCCCCAATGGGGGAAGCAGGTATCGTTCC
TGAAAATACCATAATATGCATGGGGCAAAGAATGATGCTACTTAGGCCCATACCAGAATATATTCACAACAAATATGTGT
TGTTAAACATATTATCCAGTAGTTTCCAAACTAGAATGATATCTCAGGCTATTGGGACTGGAGTAAAACATTTGCGTGTG
GCTGATGTTGAGTCATTAACATATCCTCTTCCACCTATAGAAGAACAACACGAAATTGTTCGCCGCGTCGAGCAACTCTT
CGCCTACGCCGACACCATAGAAAAACAGGTCAACAACGCCTTAGCCCGCGTCAACAACCTGACGCAATCCATCCTGGCAA
AAGCGTTCCGTGGTGAACTTACCGCCCAGTGGCGGGCCGAAAACCCGGATTTGATCAGCGGAGAAAACAGCGCCGCCGCG
TTGCTGGAAAAAATCAAAGCTGAACGCGCAGCTAGCGGGGGTAAAAAAGCCTCACGTAAAAAATCCTGA

Upstream 100 bases:

>100_bases
CGCTGGGTGAACTGGATGCGCTGATGCGTGAACTGGGGGCGAGCGATGAGGCGGATGCGCAGCGTCAGTTGCTGGAAGAA
GCGTTTGGTGGGGTGAAGGA

Downstream 100 bases:

>100_bases
ACATTATTTTCTGGCGCACCTTTCCGGTGCGCTTTTTATTATTTCACGCCAATCATAACCCACATAAATATATTTAAATC
ACTCCAGAAATTGCCCATTT

Product: Specificity determinant for hsdM and hsdR

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSAGKLPEGWVETNLQNVASWGSGGTPSRNHDEYYNGNIPWIKTGDLGPKIITNASEYITDAGVQNSSAKFFPKGSVAIA
MYGATIGKTSILGIDATTNQACAVGTPLEGITSTLFLYYFLLNEKNAFIKKGKGGAQPNISQTVIKEHIIYLPPLAEQKI
ITEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLERAVNGKLTECWRDCVGELTSAEEIITEIKKYRKASLSTEGSSAST
ESKRQIAKIEKHCFKVPKINLPKGWVWTTFLQSMEKVVDCHNKTAPYVDQGIHLIRTPDIRNGVISLDNTKYIDNDTYLY
WSKRCPPRSGDIIFTREAPMGEAGIVPENTIICMGQRMMLLRPIPEYIHNKYVLLNILSSSFQTRMISQAIGTGVKHLRV
ADVESLTYPLPPIEEQHEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAA
LLEKIKAERAASGGKKASRKKS

Sequences:

>Translated_502_residues
MSAGKLPEGWVETNLQNVASWGSGGTPSRNHDEYYNGNIPWIKTGDLGPKIITNASEYITDAGVQNSSAKFFPKGSVAIA
MYGATIGKTSILGIDATTNQACAVGTPLEGITSTLFLYYFLLNEKNAFIKKGKGGAQPNISQTVIKEHIIYLPPLAEQKI
ITEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLERAVNGKLTECWRDCVGELTSAEEIITEIKKYRKASLSTEGSSAST
ESKRQIAKIEKHCFKVPKINLPKGWVWTTFLQSMEKVVDCHNKTAPYVDQGIHLIRTPDIRNGVISLDNTKYIDNDTYLY
WSKRCPPRSGDIIFTREAPMGEAGIVPENTIICMGQRMMLLRPIPEYIHNKYVLLNILSSSFQTRMISQAIGTGVKHLRV
ADVESLTYPLPPIEEQHEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAA
LLEKIKAERAASGGKKASRKKS
>Mature_501_residues
SAGKLPEGWVETNLQNVASWGSGGTPSRNHDEYYNGNIPWIKTGDLGPKIITNASEYITDAGVQNSSAKFFPKGSVAIAM
YGATIGKTSILGIDATTNQACAVGTPLEGITSTLFLYYFLLNEKNAFIKKGKGGAQPNISQTVIKEHIIYLPPLAEQKII
TEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLERAVNGKLTECWRDCVGELTSAEEIITEIKKYRKASLSTEGSSASTE
SKRQIAKIEKHCFKVPKINLPKGWVWTTFLQSMEKVVDCHNKTAPYVDQGIHLIRTPDIRNGVISLDNTKYIDNDTYLYW
SKRCPPRSGDIIFTREAPMGEAGIVPENTIICMGQRMMLLRPIPEYIHNKYVLLNILSSSFQTRMISQAIGTGVKHLRVA
DVESLTYPLPPIEEQHEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAAL
LEKIKAERAASGGKKASRKKS

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=527, Percent_Identity=37.7609108159393, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 55713; Mature: 55581

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGKLPEGWVETNLQNVASWGSGGTPSRNHDEYYNGNIPWIKTGDLGPKIITNASEYIT
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHH
DAGVQNSSAKFFPKGSVAIAMYGATIGKTSILGIDATTNQACAVGTPLEGITSTLFLYYF
HCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHH
LLNEKNAFIKKGKGGAQPNISQTVIKEHIIYLPPLAEQKIITEKLDTLLAQVDSTKARLE
HHCCCCHHHCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIPQILKRFRQAVLERAVNGKLTECWRDCVGELTSAEEIITEIKKYRKASLSTEGSSAST
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCH
ESKRQIAKIEKHCFKVPKINLPKGWVWTTFLQSMEKVVDCHNKTAPYVDQGIHLIRTPDI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCC
RNGVISLDNTKYIDNDTYLYWSKRCPPRSGDIIFTREAPMGEAGIVPENTIICMGQRMML
CCCEEEECCCEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCHHHH
LRPIPEYIHNKYVLLNILSSSFQTRMISQAIGTGVKHLRVADVESLTYPLPPIEEQHEIV
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECHHHCCCCCCCCCHHHHHH
RRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCHHHH
LLEKIKAERAASGGKKASRKKS
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAGKLPEGWVETNLQNVASWGSGGTPSRNHDEYYNGNIPWIKTGDLGPKIITNASEYIT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHH
DAGVQNSSAKFFPKGSVAIAMYGATIGKTSILGIDATTNQACAVGTPLEGITSTLFLYYF
HCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHH
LLNEKNAFIKKGKGGAQPNISQTVIKEHIIYLPPLAEQKIITEKLDTLLAQVDSTKARLE
HHCCCCHHHCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIPQILKRFRQAVLERAVNGKLTECWRDCVGELTSAEEIITEIKKYRKASLSTEGSSAST
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCH
ESKRQIAKIEKHCFKVPKINLPKGWVWTTFLQSMEKVVDCHNKTAPYVDQGIHLIRTPDI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCC
RNGVISLDNTKYIDNDTYLYWSKRCPPRSGDIIFTREAPMGEAGIVPENTIICMGQRMML
CCCEEEECCCEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCHHHH
LRPIPEYIHNKYVLLNILSSSFQTRMISQAIGTGVKHLRVADVESLTYPLPPIEEQHEIV
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECHHHCCCCCCCCCHHHHHH
RRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCHHHH
LLEKIKAERAASGGKKASRKKS
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]