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The map label for this gene is hpaI [H]

Identifier: 218550375

GI number: 218550375

Start: 3141797

End: 3142726

Strand: Direct

Name: hpaI [H]

Synonym: EFER_3067

Alternate gene names: 218550375

Gene position: 3141797-3142726 (Clockwise)

Preceding gene: 218550374

Following gene: 218550376

Centisome position: 68.47

GC content: 58.6

Gene sequence:

>930_bases
ATGACGTACAACTGGAAGCCGGACAAATCATTCTCGGCGGCTCGTTCACCCGACCGGTTCCGGCGCGTAAAGGCGACACC
TTTCACGTCGATTACGGCAACATGGGTTCCATTAGCTGCCGCTTTGTTTAAGGAGAGAACGATGGAAAACAGCTTTAAAG
CGGCGCTGAAAGCAGGCCGACCACAGATCGGATTATGGCTGGGGCTGAGTAGTAGCTACAGTGCAGAGTTACTGGCCGGA
GCAGGATTCGACTGGTTGTTGATAGACGGTGAACACGCGCCGAATAACGTGCAAACAGTGCTCACCCAGCTACAGGCGAT
TGCGCCCTACCCCAGCCAGCCGGTAGTACGTCCGTCGTGGAACGATCCGGTGCAAATCAAACAACTGCTGGACGTCGGCA
CACAAACCTTACTGGTGCCGATGGTACAAAACGCCGACGAAGCCCGTGAAGCGGTACGCGCCACCCGTTATCCCCCCGCC
GGTATTCGTGGTGTGGGCAGTGCGCTGGCACGCGCCTCACGCTGGAATCGCATTCCTGATTACCTGCAAAAAGCCAATGA
TCAAATGTGCGTGCTGGTGCAGATCGAAACGCGTGAGGCAATGAAGAACCTACCGCAGATTCTGGACGTGGAAGGTGTCG
ACGGCGTGTTTATCGGCCCGGCGGATCTGAGCGCCGATATGGGTTATGCCGGTAATCCGCAGCACCCGGAAGTGCAGGCC
GCGATTGAACAGGCGATCGTGCAGATCCGTGAAGCGGGCAAAGCGCCGGGGATCCTGATCGCCAATGAGCAACTGGCAAA
ACGCTATCTGGAACTGGGCGCGCTGTTTGTCGCCGTCGGCGTTGACACCACCCTGCTCGCCCGCGCCGCCGAAGCACTGG
CAGCACGGTTTGGCGCGCAGGCCACGACCGTGAAGCCCGGCGTGTATTAA

Upstream 100 bases:

>100_bases
ATGTATCGCAATGGCGTGATTGAAGAAACCGGCGTCGCTGCTGGTGTGCTAAATCATCCGGCAAACGGCGTGGCCTGGCT
GGCGAACAAACTCGCACCGT

Downstream 100 bases:

>100_bases
TGCCGGATGCGCTGCGCTTATCCAGCCTACACGCGCACCAAACCGTAGGCCGGATAAGGCGTTTGTGCTGCATCTGCCAT
AAACCTTGTCGTACCCTACA

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTYNWKPDKSFSAARSPDRFRRVKATPFTSITATWVPLAAALFKERTMENSFKAALKAGRPQIGLWLGLSSSYSAELLAG
AGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPA
GIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQA
AIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY

Sequences:

>Translated_309_residues
MTYNWKPDKSFSAARSPDRFRRVKATPFTSITATWVPLAAALFKERTMENSFKAALKAGRPQIGLWLGLSSSYSAELLAG
AGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPA
GIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQA
AIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY
>Mature_308_residues
TYNWKPDKSFSAARSPDRFRRVKATPFTSITATWVPLAAALFKERTMENSFKAALKAGRPQIGLWLGLSSSYSAELLAGA
GFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAG
IRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAA
IEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=56.4, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=48.3870967741936, Blast_Score=233, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 33489; Mature: 33358

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNWKPDKSFSAARSPDRFRRVKATPFTSITATWVPLAAALFKERTMENSFKAALKAGR
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSW
CCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPD
CCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHH
YLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQA
HHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHH
AIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGAQ
HHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ATTVKPGVY
CCCCCCCCC
>Mature Secondary Structure 
TYNWKPDKSFSAARSPDRFRRVKATPFTSITATWVPLAAALFKERTMENSFKAALKAGR
CCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSW
CCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPD
CCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHH
YLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQA
HHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHH
AIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGAQ
HHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ATTVKPGVY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]