Click here to switch to the map view.

The map label for this gene is rfaE [H]

Identifier: 218550304

GI number: 218550304

Start: 3064510

End: 3065943

Strand: Reverse

Name: rfaE [H]

Synonym: EFER_2996

Alternate gene names: 218550304

Gene position: 3065943-3064510 (Counterclockwise)

Preceding gene: 218550305

Following gene: 218550300

Centisome position: 66.81

GC content: 56.07

Gene sequence:

>1434_bases
ATGAAAGTAACGCTGCCAGAGTTTGAACGTGCAGGAGTGATGGTGGTTGGTGATGTGATGCTGGATCGTTACTGGTACGG
TCCCACCAGCCGTATCTCACCGGAAGCGCCAGTACCAGTGGTTAAAGTGAATACCATCGAAGAACGTCCGGGCGGCGCGG
CCAACGTGGCGATGAACATCGCTTCTCTCGGTGCAAACGCACGCCTGGTCGGGTTGACGGGCATTGACGATGCGGCGCGC
GCGCTGAGTAAGTCTCTGGCCGACGTCAACGTCAAATGCGACTTCGTTTCTGTACCGACGCATCCGACCATCACCAAACT
GCGGGTGCTTTCCCGCAACCAACAGCTGATCCGCCTGGATTTCGAAGAAGGTTTTGAAGGCGTTGATCCGCAACCGCTCC
ATGAGCGGATTAATCAGGCGCTGAGTTCGATTGGTGCGCTGGTACTTTCGGACTACGCGAAAGGTGCGCTGGCAAGCGTA
CAGCAGATGATCCAACTGGCGCGTAAAGCGGGCGTTCCGGTGCTGATTGATCCAAAAGGGACCGATTTTGAGCGCTACCG
CGGCGCTACGCTGTTAACGCCGAATCTCTCGGAATTTGAAGCTGTTGTTGGTAAATGTAAAACAGAAGAAGAGATTGTTG
AACGCGGCATGAAACTGATTGCCGACTACGAACTCTCTGCGCTGCTGGTAACCCGTTCCGAGCAGGGGATGTCGCTGCTG
CAACCGGGTAAAGAGCCGCTGCATATGCCAACCCAGGCGCAAGAAGTGTATGACGTCACCGGTGCGGGCGATACGGTGAT
TGGTGTGCTGGCAGCAACGCTGGCGGCGGGTAATTCGCTGGAAGAAGCCTGCTTCTTTGCCAACGCGGCGGCTGGCGTGG
TGGTTGGCAAACTGGGAACATCCACGGTTTCACCGATCGAGCTGGAAAACGCAGTACGTGGACGTGCAGATACCGGCTTT
GGTGTGATGACCGAAGAGGAGCTGAAGCTGGCGGTAGCGGCGGCGCGTAAACGCGGTGAAAAAGTGGTGATGACCAACGG
CGTCTTCGACATTCTGCACGCCGGGCATGTCTCCTATCTGGCAAACGCCCGTAAACTGGGTGACCGTTTGATTGTCGCCG
TCAACAGCGACGCCTCCACCAAACGGCTGAAAGGGGATTCCCGCCCCGTTAACCCGCTCGAACAGCGTATGATTGTGCTG
GGCGCACTGGAAGCGGTCGACTGGGTGGTGTCGTTTGAAGAAGACACGCCGCAGCGCCTGATCGCGGGGATCTTGCCAGA
TCTGCTGGTGAAAGGCGGCGACTATAAACCAGAAGAGATTGCCGGAAGTAAAGAAGTCTGGGCCAATGGTGGCGAAGTGC
TGGTGCTCAACTTCGAAGATGGTTGTTCGACCACTAACATTATCAAGAAGATCCAACAGGATAAAAAAGGCTAA

Upstream 100 bases:

>100_bases
CAGAGCGTGAACTGGTGAGGGCAAGCTGGCAGAAGTGGCTGGTGGAAGAATGAAGTATGGTATTATCGCGCGCAAATTTT
GAATCTCTCAGGAGACAGGA

Downstream 100 bases:

>100_bases
CCGGAAAGCGGTTCACAGTTCTCGCTTCGTACCTGGCGGGCCTATTGACGCTGTTGTTTTTTCAACCTAGAGTAAAGGAA
CAAGGGTAAGGGAGGATTTC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKEPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG

Sequences:

>Translated_477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKEPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
>Mature_477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKEPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=477, Percent_Identity=99.79035639413, Blast_Score=964, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 51109; Mature: 51109

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
ASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
QPGKEPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
HHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
ASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
QPGKEPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
HHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA