Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 218550279

GI number: 218550279

Start: 3039733

End: 3041625

Strand: Reverse

Name: parE [H]

Synonym: EFER_2971

Alternate gene names: 218550279

Gene position: 3041625-3039733 (Counterclockwise)

Preceding gene: 218550280

Following gene: 218550274

Centisome position: 66.28

GC content: 52.51

Gene sequence:

>1893_bases
ATGACGCAAACTTATAACGCTGATGCCATTGAGGTACTCACCGGGCTTGAGCCGGTACGCCGCCGCCCGGGGATGTATAC
CGATACCACTCGCCCAAACCATTTAGGGCAAGAAGTTATTGATAACAGTGTGGATGAAGCACTGGCGGGTCACGCAAAAC
GCGTGGACGTAATTTTACATGCTGACCAGTCGTTAGAAGTTATTGACGATGGTCGTGGGATGCCGGTGGATATTCACCCG
GAAGAGGGTGTGCCGGCGGTCGAATTGATTCTCTGCCGTCTGCACGCGGGCGGTAAGTTTTCTAACAAAAACTACCAGTT
CTCCGGTGGTCTTCACGGCGTGGGGATCTCGGTGGTTAACGCCCTGTCGAAACGCGTAGAAGTTAACGTGCGACGCGATG
GTCAGGTCTACAACATCGCGTTTGAGAACGGCGAAAAGGTGCAGGATCTGCAAGTTGTTGGCAACTGTGGTAAACGCAAT
ACCGGCACCAGCGTGCACTTCTGGCCGGATGAAACCTTCTTTGACAGCCCACGCTTTTCTGTTTCGCGCCTGACACATGT
CCTGAAAGCCAAAGCGGTACTGTGCCCTGGTGTTGAGATCACCTTTAAAGATGAAATCAACAACACCGAACAACGCTGGT
GCTATCAGGACGGCCTGAATGACTACCTGGCGGAAGCGGTAAATGGCTTACCGACACTGCCGGAAAAACCGTTTATTGGT
AATTTTTCGGGTGAAACGGAAGCCGTGGACTGGGCATTGTTATGGTTGCCGGAAGGCGGAGAACTGCTGACAGAAAGCTA
CGTCAACCTGATCCCCACCATGCAGGGCGGTACCCATGTTAATGGTTTGCGTCAGGGCCTTCTGGATGCGATGCGTGAGT
TTTGTGAATACCGCAATATCCTGCCGCGTGGTGTGAAGTTGTCGGCGGAAGACATCTGGGATCGCTGCGCTTACGTGCTA
TCAGTGAAAATGCAGGACCCGCAGTTTGCCGGGCAAACCAAAGAACGTCTCTCGTCACGTCAATGTGCAGCGTTTGTTTC
TGGCGTGGTGAAAGATGCCTTCAGTTTGTGGCTAAACCAGAACGTGCAGGCGGCTGAGCTGTTGGCAGAAATGGCGATCT
CCAGCGCCCAGCGTCGTATGCGTGCGGCGAAGAAGGTTGTGCGTAAAAAGCTGACCAGCGGCCCGGCACTGCCTGGCAAA
CTGGCTGACTGTACTGCGCAGGATCTTAATCGTACCGAACTGTTCCTCGTCGAAGGTGACTCCGCAGGCGGATCTGCCAA
GCAGGCGCGCGATCGTGAATATCAGGCGATCATGCCGTTGAAAGGTAAGATCCTTAACACCTGGGAAGTCTCTTCTGATG
AAGTGCTTGCTTCGCAGGAAGTGCACGATATTTCGGTAGCGATCGGTATCGATCCTGATAGTGACGATCTCAGCCAGCTG
CGTTACGGTAAGATCTGTATCCTCGCGGATGCGGACTCCGATGGTCTACACATTGCCACGCTGCTTTGCGCACTGTTTGT
AAAACACTTCCGCACGTTGGTGAAACACGGTCACGTTTACGTTGCTCTGCCACCGCTCTACCGTATTGACCTTGGAAAAG
AGGTTTATTACGCGCTGACAGAAGAAGAGAAAGAGGGCGTGCTCGAGCAATTAAAACGCAAGAAAGGCAAGCCGAACGTT
CAGCGTTTTAAAGGTCTGGGGGAAATGAACCCGATGCAATTGCGCGAAACCACGCTTGATCCGAATACTCGCCGTCTGGT
GCAGTTGACTATCGATGATGAAGATGATCAGCGTACTGATGCCGTCATGGATATGCTTCTGGCGAAAAAACGTTCAGAAG
ATCGTCGCAACTGGTTACAGGAAAAAGGCGACATGGCGGAGATTGAAGTTTAA

Upstream 100 bases:

>100_bases
AACCACGCATTCACGGGCTTCGAAGATTATTTCAACCCGATCGTCGATTTTCTTGGACTGCATAATTTCTGACGATCAAC
TCGAATTGCTAACTTAAACC

Downstream 100 bases:

>100_bases
AGAAAAGATCATTGCCCGGTCAGAGTGGCCGGGCAAAGCGATCAGGATTAAATCCCGGACTGCAAAATACGAATATTGGT
TTCCAGTACGTCACCTTTTA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRTLVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAVMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Sequences:

>Translated_630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRTLVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAVMDMLLAKKRSEDRRNWLQEKGDMAEIEV
>Mature_629_residues
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPE
EGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNT
GTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGN
FSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLS
VKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKL
ADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLR
YGKICILADADSDGLHIATLLCALFVKHFRTLVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQ
RFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAVMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=577, Percent_Identity=24.9566724436742, Blast_Score=130, Evalue=6e-30,
Organism=Homo sapiens, GI19913406, Length=581, Percent_Identity=24.0963855421687, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=99.0476190476191, Blast_Score=1295, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=41.0810810810811, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI212645845, Length=605, Percent_Identity=24.4628099173554, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17535065, Length=605, Percent_Identity=24.297520661157, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=647, Percent_Identity=24.2658423493045, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI17136538, Length=584, Percent_Identity=22.9452054794521, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70260; Mature: 70128

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRTLVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AVMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRTLVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AVMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]