Click here to switch to the map view.

The map label for this gene is hybF [H]

Identifier: 218550242

GI number: 218550242

Start: 3001793

End: 3002134

Strand: Reverse

Name: hybF [H]

Synonym: EFER_2934

Alternate gene names: 218550242

Gene position: 3002134-3001793 (Counterclockwise)

Preceding gene: 218550243

Following gene: 218550241

Centisome position: 65.42

GC content: 54.68

Gene sequence:

>342_bases
ATGCATGAGTTGTCGCTTTGCCAGAGCGCCGTTGAAATTATCCAACGGCAGGCGGAGCAGCATGATGTTAAGCGCGTCAC
CGCCGTGTGGCTGGAAATTGGCGCGCTCTCCTGCGTTGAGGAGAGCGCTGTCCGTTTTAGTTTTGAAATTGTCTGCCAGG
GAACGGTGGCCCAAGGGTGCGATTTACATATCGTCTATAAACCCGCCCAGGCCTGGTGCTGGGATTGCAGCCAGGTGGTG
GAGATTCATCAGCACGATGCGCAATGCCCGCTCTGTCATGGCGAGCGGTTGCGTGTCGATACCGGCGATTCGCTGATCGT
CAAAAGTATTGAAGTTGAATAA

Upstream 100 bases:

>100_bases
GATGACTGCGCGCGGATGATCCTTTCGCTGCCGGTCACCAATCCGGATGTACCACACGCAGGGCGTCGTGCCCTGCTATT
TGGCCGCAGGAGTGGCGAAA

Downstream 100 bases:

>100_bases
CCGGAGTTAACAATGTGTATTGGCGTTCCAGGCCAGGTGCTGGCTGTCGGTGAAGATATTCACCAGCTTGCGCAGGTTGA
AGTATGTGGTATCAAGCGCG

Product: hydrogenase nickel incorporation protein HybF

Products: NA

Alternate protein names: Hydrogenase-2 operon protein hybF [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCQGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Sequences:

>Translated_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCQGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
>Mature_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCQGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step [H]

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family [H]

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=99.1150442477876, Blast_Score=225, Evalue=6e-61,
Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=48.2456140350877, Blast_Score=118, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000688
- InterPro:   IPR020538 [H]

Pfam domain/function: PF01155 HypA [H]

EC number: NA

Molecular weight: Translated: 12689; Mature: 12689

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCQGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCQGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]