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The map label for this gene is gss [H]

Identifier: 218550238

GI number: 218550238

Start: 2997489

End: 2999348

Strand: Reverse

Name: gss [H]

Synonym: EFER_2930

Alternate gene names: 218550238

Gene position: 2999348-2997489 (Counterclockwise)

Preceding gene: 218550241

Following gene: 218550237

Centisome position: 65.36

GC content: 48.55

Gene sequence:

>1860_bases
ATGAGCAAAGGAACGACCAGTCAGGATGCCCCCTTTGGGACATTATTGGGCTACGCCCCCGGTGGTGTAGCTATCTACTC
ATCAGATTACAGCTCCCTTGATCCACGGGAATATGAAGATGATTCCGCTTTTCGCAGCTACATCGATGATGAATATATGG
GGCACAAATGGCAGTGCGTTGAGTTCGCCCGCCGTTTTCTCTTTCTTAACTATGGGGTAGTGTTCACTGACGTAGGGATG
GCGTGGGAGATTTTCTCGCTGCGTTTTCTGCGTGAAGTGGTGAATGACAACATCCTGCCATTGCAGGCATTTCCGAACGG
TTCACCGCGTGCGCCAGTCGCTGGTGCGTTACTTATCTGGGATAAAGGCGGTGAATTTAAAGATACCGGTCATGTTGCCA
TTATTACCCAATTGCATGGCAATAAAGTCCGCATCGCCGAACAGAATGTGATCCACACGCCGCTTCCTCCTGGGCAGCAA
TGGACACGTGAACTGGAGATGGTTGTGGAGAATGGGCGTTATACTCTGCGCGACACCTTCGATGACACGACGATTTTGGG
CTGGATGATCCAGACTGATGATACGCAACACAGCCTGCCACAACCGGAGATTGCCAGTGATGCGCTGAAGATTCGTGGTG
CTCGTCTGGAAGATAAAGGTCAGTTTGACGGGAAATGGCTGGATGAGCAGGATCCACTACAAAACGCTTACGTTCAGGCG
AACGGTCAGGTGATTAACCAGGACCCATGTCAGTATTTCACCATTACCGAAAGCGCCGAGCAGGAGTTAATCAAAGCAAC
CAACGAGTTGCATTTAATGTATCTTCACGCCACTGACAAAGTGCTGAAAGATGATAACTTATTAGCGCTATTTGATATTC
CCAAAATTCTTTGGCCACGTTTACGTCTTTCCTGGCAACGTCGTCGTCACCATATGATCACCGGGCGCATGGACTTTTGT
ATGGATGAGCGCGGGCTAAAGGTCTATGAATACAACGCTGATTCTGCTTCCTGCCATACCGAAGCTGGATTGATTCTTGA
GAAGTGGGCGGAGCAGGGCTACAAAGGCAACGGTCATAACCCGGCTGAGGGCTTATTGAACGAGCTGGCAGGGGCATGGA
AACATAGCCGTGCACGACCTTTTGTGCATATTATGCAAGATGATGATATCGAAGAGAATTATCACGCGCAGTTTATGCAG
CAGGCACTTCATCAGGCAGGATTTGAAACGAAAATAGTGCGCGGACTGGCAGAATTACGCTGGGATGATGCCGGGCAGCT
GATTGACGGCGAAGGCCGGTTGGTCAATTGCGTCTGGAAAACCTGGGCGTGGGAAACCGCCTTCGACCAAATCCGGGAAG
TGAGTGACAAAGAGTTTGCTGCGGTTCCAATTCGTACAGGTCATCCGCAAAATGAGGTGCGCCTGATCGATGTTCTGCTG
CGTCCAGAAGTGCTGGTATTTGAGCCTTTATGGACAGTAATTCCAGGAAATAAAGCGATATTACCTATCTTATGGTCGCT
TTTCCCGCATCATCGTTACTTACTGGACACTGATTTTATGGTAAACGACGAACTGATCCGTACGGGCTATGCTGTGAAGC
CAATTGCCGGTCGGTGCGGGAGCAATATTGATCTGGTGAGCCACCAGGAAGAGTTGCTTGATAAGACCAGCGGTAAGTTT
GCAGAGCAAAAAAATATTTACCAGCAACTATGGTGCTTACCAAACGTAGCCGGAAAATATATTCAGGTCTGTACATTTAC
GGTTGGTGGTAATTATGGTGGCACATGCTTACGCGGTGATGATTCTCTGGTTATTAAAAAAGAGAGTGATATTGAACCGT
TAATTGTGTTGAAAGAGTAA

Upstream 100 bases:

>100_bases
ATGATAAATGGACCTCTGTCCGGTAATCGTCGCTGAGCAGTAAAACAGAGCACAACAGTCACGCCAGACTGCTCAGGTTG
TAATGTTAAGGTTAAATTAC

Downstream 100 bases:

>100_bases
TTACGGAAATGAATAGCCCGCCGGTAAGGCGGGCTGAGACTAATGATAAATAAAGGATTGCCTGATGCACTGCGCTTACC
AGACCAATAAACAGGCTATT

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPREYEDDSAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPPGQQ
WTRELEMVVENGRYTLRDTFDDTTILGWMIQTDDTQHSLPQPEIASDALKIRGARLEDKGQFDGKWLDEQDPLQNAYVQA
NGQVINQDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILEKWAEQGYKGNGHNPAEGLLNELAGAWKHSRARPFVHIMQDDDIEENYHAQFMQ
QALHQAGFETKIVRGLAELRWDDAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDKEFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFMVNDELIRTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKF
AEQKNIYQQLWCLPNVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVLKE

Sequences:

>Translated_619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPREYEDDSAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPPGQQ
WTRELEMVVENGRYTLRDTFDDTTILGWMIQTDDTQHSLPQPEIASDALKIRGARLEDKGQFDGKWLDEQDPLQNAYVQA
NGQVINQDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILEKWAEQGYKGNGHNPAEGLLNELAGAWKHSRARPFVHIMQDDDIEENYHAQFMQ
QALHQAGFETKIVRGLAELRWDDAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDKEFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFMVNDELIRTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKF
AEQKNIYQQLWCLPNVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVLKE
>Mature_618_residues
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPREYEDDSAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
WEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPPGQQW
TRELEMVVENGRYTLRDTFDDTTILGWMIQTDDTQHSLPQPEIASDALKIRGARLEDKGQFDGKWLDEQDPLQNAYVQAN
GQVINQDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILEKWAEQGYKGNGHNPAEGLLNELAGAWKHSRARPFVHIMQDDDIEENYHAQFMQQ
ALHQAGFETKIVRGLAELRWDDAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDKEFAAVPIRTGHPQNEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFMVNDELIRTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKFA
EQKNIYQQLWCLPNVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVLKE

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=619, Percent_Identity=93.5379644588045, Blast_Score=1215, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=376, Percent_Identity=26.063829787234, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790629, Length=370, Percent_Identity=26.7567567567568, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 70528; Mature: 70396

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPREYEDDSAFRSYIDDEYMGHKWQCV
CCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPPGQQWTRELEMVVENGRYTLRDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCEEEEECC
DDTTILGWMIQTDDTQHSLPQPEIASDALKIRGARLEDKGQFDGKWLDEQDPLQNAYVQA
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILEKWAEQGYKGNGHN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PAEGLLNELAGAWKHSRARPFVHIMQDDDIEENYHAQFMQQALHQAGFETKIVRGLAELR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDDAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDKEFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHHCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFMVNDELIRTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCEECHHHHHCCCEECCCHHCCC
SNIDLVSHQEELLDKTSGKFAEQKNIYQQLWCLPNVAGKYIQVCTFTVGGNYGGTCLRGD
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECC
DSLVIKKESDIEPLIVLKE
CCEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPREYEDDSAFRSYIDDEYMGHKWQCV
CCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPPGQQWTRELEMVVENGRYTLRDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCEEEEECC
DDTTILGWMIQTDDTQHSLPQPEIASDALKIRGARLEDKGQFDGKWLDEQDPLQNAYVQA
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILEKWAEQGYKGNGHN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PAEGLLNELAGAWKHSRARPFVHIMQDDDIEENYHAQFMQQALHQAGFETKIVRGLAELR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDDAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDKEFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHHCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFMVNDELIRTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCEECHHHHHCCCEECCCHHCCC
SNIDLVSHQEELLDKTSGKFAEQKNIYQQLWCLPNVAGKYIQVCTFTVGGNYGGTCLRGD
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECC
DSLVIKKESDIEPLIVLKE
CCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]