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The map label for this gene is aas [H]

Identifier: 218550085

GI number: 218550085

Start: 2835813

End: 2837972

Strand: Reverse

Name: aas [H]

Synonym: EFER_2770

Alternate gene names: 218550085

Gene position: 2837972-2835813 (Counterclockwise)

Preceding gene: 218550086

Following gene: 218550084

Centisome position: 61.85

GC content: 51.16

Gene sequence:

>2160_bases
ATGCTTTTCGGCTTTTTTCGTAAATTATGTCAGGTGTTATACCGTGTTCAGGTCACGGGAGACCCAGCGGCACTAAAAGG
TGAGCGTGTGCTCATCACACCAAACCATGTTTCCTTTATTGATGGCATCTTGCTGGCGCTGTTTTTACCTGTGCGCCCGG
TGTTTGCCGTGTACACATCCATCAGCCAGCAGTGGTATATGCGCTGGTTACAATCCCTTATCGACTTTGTACCGCTGGAC
CCCACCAAACCGATGGCGATTAAACATCTGGTGCGTCTGGTAGAACAGGGGCGCCCGGTGGTAATTTTTCCGGAAGGGAG
AATCACAGTAACCGGTTCGCTGATGAAGATATACGATGGTGCGGGTTTTGTGGCAGCAAAATCAGGCGCGACGGTCGTTC
CAGTTCGTATTGAAGGGGCTGAATTAACTCATTTCAGCCGTCTGAAAGGTCTGGTCAAACGTCGATTGTTCCCGAAGATT
CATCTGCATATTTTGCCACCAACTCATGTGCCGATGCCGGATGCGCCACGAGCGCGCGATCGCCGTAAAATCGCGGGCGA
AATGCTGCACCAGATCATGATGGAAGCGCGGATGGCCGTGCGTCCACGTGAAACGCTGTATGAATCGTTATTGGGAGCTA
TGCACCGCTACGGTGGCGGAAAAAATTGCGTTGAAGATGTCAATTTTACCCCGGACAGCTATCGAAAATTACTCACCAAG
ACGCTGTTTGTCGGGCGTATTCTTGAGAAGTACAGTGCAGAAGGGGAAAGAATCGGCTTAATGCTACCCAATGCAGGTAT
TAGCGCAGCGGTGATTTTCGGGGCCATTGCCCGTCGCCGTATCCCGGCAATGATGAACTACACCGCCGGCGTAAAAGGGC
TAACCAGTGCTATCACTGCCGCTGAAATCAAAACTATTTTCACCTCTCGCCAGTTCCTTGATAAAGGCAAACTCTGGCAT
CTGCCGGAACAACTCACACAGGTTCGTTGGATATATCTGGAAGACCTGAAAGCCGACGTTACCTTGACAGACAAAGTCTG
GATCTTTGCTCATTTATTAATGCCGCATCTGGCACAGGTAAAACAGCAACCCGAGGAAGAGGCGTTAATTCTCTTTACTT
CTGGTTCTGAAGGTCATCCTAAAGGGGTGGTTCATAGCCATAAAAGCATTCTGGCAAACGTTGAGCAGATTAAAACTATC
GCCGACTTCACCACCAACGACCGCTTTATGTCGGCGTTACCGCTGTTTCACTCTTTTGGTCTGACGGTGGGGCTTTTTAC
TCCTTTACTGACAGGTGCCGAAGTCTTCCTTTACCCAAGCCCGCTACACTATCGCATTGTGCCGGAGCTGGTGTATGACC
GTAATTGCACAGTGCTTTTCGGCACATCAACTTTCCTCGGTCATTACGCGCGCTTTGCCAACCCGTATGATTTCTATCGC
CTACGCTATGTGGTGGCTGGTGCAGAAAAACTGCAAGAAAGTACCAAACAGCTTTGGCAGGATAAATTTGGCCTGCGAAT
TCTCGAAGGTTATGGCGTTACCGAATGTGCGCCGGTGGTCTCTATCAACGTGCCGATGGCAGCCAAACCGGGCACTGTCG
GGCGTATTCTGCCAGGAATGGATGCCCGCTTGTTGGCAGTGCCAGGTATTGAAGACGGTGGGCGTTTGCAACTGAAAGGG
CCAAATATCATGAATGGCTACTTGCGGGTGGAAAAACCTGGCGTGCTGGAAGTGCCAACCGCAGAGAATATCCAGGGTGA
GCTGGAACGCGGCTGGTATGACACTGGCGATATCGTGCGTTTTGACGAGCAAGGGTTTGTGCAGATTCAGGGGCGGGCGA
AACGCTTTGCTAAAATTGCCGGGGAGATGGTGTCACTGGAAATGGTTGAACAACTGGCACTGGGGGTTTCGCCTGATAAA
GTTCACGCCACCGCAATTAAGAGCGATGCCAGCAAAGGTGAGGCACTGGTGCTGTTCACCACCGACAGCGAGCTGACGCG
TGACAAACTGCAACAATACGCCCGCGAGCACGGTGTACCAGAACTTGCTGTACCACGCGATATTCGCTATCTGAAACAGA
TGCCGCTGTTGGGCAGTGGTAAGCCAGACTTTGTGACACTAAAAAGCTGGGTCGACGAGCCGGAAAAACATAATGAGTGA

Upstream 100 bases:

>100_bases
TTTTTTTCGCCTCATTTTTTCTCTTGCTCGCATTCCAATCCTCTTCTCGTGTAATCTGCCTTCATTCGTTTTTTCTGAAT
ATTCTGCAAAGGGAGTTGAT

Downstream 100 bases:

>100_bases
GTCAGTGCACACTAACACTTCGCTATGGTCGAAGGGGATGAAGGCCGTTATCGTCGCGCAGTTTCTTTCTGCATTTGGTG
ATAACGCGCTACTGTTTGCC

Product: bifunctional acyl-[acyl carrier protein] synthetase/2-acylglycerophosphoethanolamine acyltransferase

Products: NA

Alternate protein names: 2-acylglycerophosphoethanolamine acyltransferase; 2-acyl-GPE acyltransferase; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase; Acyl-[acyl-carrier-protein] synthetase; Acyl-ACP synthetase; Long-chain-fatty-acid--[acyl-carrier-protein] ligase [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MLFGFFRKLCQVLYRVQVTGDPAALKGERVLITPNHVSFIDGILLALFLPVRPVFAVYTSISQQWYMRWLQSLIDFVPLD
PTKPMAIKHLVRLVEQGRPVVIFPEGRITVTGSLMKIYDGAGFVAAKSGATVVPVRIEGAELTHFSRLKGLVKRRLFPKI
HLHILPPTHVPMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLGAMHRYGGGKNCVEDVNFTPDSYRKLLTK
TLFVGRILEKYSAEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWH
LPEQLTQVRWIYLEDLKADVTLTDKVWIFAHLLMPHLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTI
ADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGHYARFANPYDFYR
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLAVPGIEDGGRLQLKG
PNIMNGYLRVEKPGVLEVPTAENIQGELERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDK
VHATAIKSDASKGEALVLFTTDSELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEPEKHNE

Sequences:

>Translated_719_residues
MLFGFFRKLCQVLYRVQVTGDPAALKGERVLITPNHVSFIDGILLALFLPVRPVFAVYTSISQQWYMRWLQSLIDFVPLD
PTKPMAIKHLVRLVEQGRPVVIFPEGRITVTGSLMKIYDGAGFVAAKSGATVVPVRIEGAELTHFSRLKGLVKRRLFPKI
HLHILPPTHVPMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLGAMHRYGGGKNCVEDVNFTPDSYRKLLTK
TLFVGRILEKYSAEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWH
LPEQLTQVRWIYLEDLKADVTLTDKVWIFAHLLMPHLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTI
ADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGHYARFANPYDFYR
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLAVPGIEDGGRLQLKG
PNIMNGYLRVEKPGVLEVPTAENIQGELERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDK
VHATAIKSDASKGEALVLFTTDSELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEPEKHNE
>Mature_719_residues
MLFGFFRKLCQVLYRVQVTGDPAALKGERVLITPNHVSFIDGILLALFLPVRPVFAVYTSISQQWYMRWLQSLIDFVPLD
PTKPMAIKHLVRLVEQGRPVVIFPEGRITVTGSLMKIYDGAGFVAAKSGATVVPVRIEGAELTHFSRLKGLVKRRLFPKI
HLHILPPTHVPMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLGAMHRYGGGKNCVEDVNFTPDSYRKLLTK
TLFVGRILEKYSAEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWH
LPEQLTQVRWIYLEDLKADVTLTDKVWIFAHLLMPHLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTI
ADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGHYARFANPYDFYR
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLAVPGIEDGGRLQLKG
PNIMNGYLRVEKPGVLEVPTAENIQGELERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDK
VHATAIKSDASKGEALVLFTTDSELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEPEKHNE

Specific function: Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ATP- dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=385, Percent_Identity=27.7922077922078, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI187761343, Length=385, Percent_Identity=27.7922077922078, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI40807491, Length=408, Percent_Identity=26.2254901960784, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI156151445, Length=369, Percent_Identity=25.4742547425474, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI42544132, Length=376, Percent_Identity=26.063829787234, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI57165410, Length=411, Percent_Identity=25.5474452554745, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI57165412, Length=411, Percent_Identity=25.3041362530414, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI157311624, Length=383, Percent_Identity=26.3707571801567, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI157311622, Length=383, Percent_Identity=26.3707571801567, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI42544134, Length=207, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1789201, Length=719, Percent_Identity=94.8539638386648, Blast_Score=1410, Evalue=0.0,
Organism=Escherichia coli, GI1788107, Length=489, Percent_Identity=27.19836400818, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI145693145, Length=358, Percent_Identity=29.8882681564246, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1786810, Length=360, Percent_Identity=22.5, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=340, Percent_Identity=27.3529411764706, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=340, Percent_Identity=27.9411764705882, Blast_Score=112, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=444, Percent_Identity=25, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=444, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=444, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510401, Length=350, Percent_Identity=25.4285714285714, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=365, Percent_Identity=24.3835616438356, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=310, Percent_Identity=27.741935483871, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17541856, Length=409, Percent_Identity=24.2053789731051, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=274, Percent_Identity=25.5474452554745, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=375, Percent_Identity=24.2666666666667, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=358, Percent_Identity=25.9776536312849, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320852, Length=163, Percent_Identity=26.3803680981595, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355181, Length=522, Percent_Identity=23.5632183908046, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24581924, Length=361, Percent_Identity=24.0997229916898, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648260, Length=394, Percent_Identity=25.1269035532995, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922652, Length=337, Percent_Identity=25.2225519287834, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF01553 Acyltransferase; PF00501 AMP-binding [H]

EC number: =2.3.1.40; =6.2.1.20 [H]

Molecular weight: Translated: 80489; Mature: 80489

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGFFRKLCQVLYRVQVTGDPAALKGERVLITPNHVSFIDGILLALFLPVRPVFAVYTS
CCHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISQQWYMRWLQSLIDFVPLDPTKPMAIKHLVRLVEQGRPVVIFPEGRITVTGSLMKIYDG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECEEEEECC
AGFVAAKSGATVVPVRIEGAELTHFSRLKGLVKRRLFPKIHLHILPPTHVPMPDAPRARD
CCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
RRKIAGEMLHQIMMEARMAVRPRETLYESLLGAMHRYGGGKNCVEDVNFTPDSYRKLLTK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHH
TLFVGRILEKYSAEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
AEIKTIFTSRQFLDKGKLWHLPEQLTQVRWIYLEDLKADVTLTDKVWIFAHLLMPHLAQV
HHHHHHHHHHHHHHCCCEECCHHHHHHEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHH
KQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTIADFTTNDRFMSALPLFHSFG
HHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
LTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGHYARFANPYDFYR
HHHHHHHHHHCCCEEEEECCCCCEEEEHHHHCCCCCEEEEECHHHHHHHHHHCCCHHHHH
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGM
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DARLLAVPGIEDGGRLQLKGPNIMNGYLRVEKPGVLEVPTAENIQGELERGWYDTGDIVR
CCEEEEECCCCCCCEEEECCCCEEECEEEECCCCEEECCCCCCCCHHHHCCCCCCCCEEE
FDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFT
ECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEE
TDSELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEPEKHNE
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHCCCCCCCCC
>Mature Secondary Structure
MLFGFFRKLCQVLYRVQVTGDPAALKGERVLITPNHVSFIDGILLALFLPVRPVFAVYTS
CCHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISQQWYMRWLQSLIDFVPLDPTKPMAIKHLVRLVEQGRPVVIFPEGRITVTGSLMKIYDG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECEEEEECC
AGFVAAKSGATVVPVRIEGAELTHFSRLKGLVKRRLFPKIHLHILPPTHVPMPDAPRARD
CCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
RRKIAGEMLHQIMMEARMAVRPRETLYESLLGAMHRYGGGKNCVEDVNFTPDSYRKLLTK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHH
TLFVGRILEKYSAEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
AEIKTIFTSRQFLDKGKLWHLPEQLTQVRWIYLEDLKADVTLTDKVWIFAHLLMPHLAQV
HHHHHHHHHHHHHHCCCEECCHHHHHHEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHH
KQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTIADFTTNDRFMSALPLFHSFG
HHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
LTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGHYARFANPYDFYR
HHHHHHHHHHCCCEEEEECCCCCEEEEHHHHCCCCCEEEEECHHHHHHHHHHCCCHHHHH
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGM
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DARLLAVPGIEDGGRLQLKGPNIMNGYLRVEKPGVLEVPTAENIQGELERGWYDTGDIVR
CCEEEEECCCCCCCEEEECCCCEEECEEEECCCCEEECCCCCCCCHHHHCCCCCCCCEEE
FDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFT
ECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEE
TDSELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEPEKHNE
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA