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The map label for this gene is rspB [C]

Identifier: 218549981

GI number: 218549981

Start: 2742594

End: 2743619

Strand: Reverse

Name: rspB [C]

Synonym: EFER_2666

Alternate gene names: 218549981

Gene position: 2743619-2742594 (Counterclockwise)

Preceding gene: 218549982

Following gene: 218549977

Centisome position: 59.79

GC content: 45.61

Gene sequence:

>1026_bases
ATGAAAGTCAAAGCTGTGTACATCAATAAACCTGGTGAAATTGCCACTCGCCTGGTCGATTATCCGGTAAAAAAAGATAA
TGAAGTTTTAATTAAAGTGGATGCAGCGGGGATTTGTGGTTCTGACATCGGTGCATTTCGTGGCACTAACCCACTGGTAA
CTTATCCGCGAATTATTGGACATGAAGTCACCGGGATTGTTCTGGAAACGGGTAAGGGGATGCCAGAAGATATTAAGCAG
GGCGACCGGGTTATTGTAGATCCCTATATTTATTGCGGTCACTGTTATCCATGTTCTGTCGGCAGAACTAATTGCTGTGA
GAACTTAAAAGTCATCGGCGTACATATTGATGGCGCTATGCAAGAAGTGGTTGCGCATCCGGCTCACTTAATTCACAAAA
TTCCTGACAATGTACCGTCAGAAATGGCGCCACTGGCTGAACCCTTAACTATTGCCCTGCACGCATTACATCGGGCGCAA
TTGAAAGCAGGTGAACATGTCGTCATTATTGGTGCTGGTGCCATTGGACTTATGGCGGCACTGAGTGCGTTGCGCTATCA
CGCAACCCCCATTCTGGTTGATATTGTCGATGAACGGTTGCAATACGCACAATCGCTCGGTGTGCCTTACGTAGTTAACT
CCGCAAAAGCTAACGTTGTTGAGGCAGTGAAAGATATTACACAAGGGACGATGGCTCAGGTTGTGGTGGAAGCCTCTGGT
GCAAATAGCGCGATCAGAAGTACCCTGGATCTGGCCTCATTTGCCGGGCGTATTGCCTATACGGGTTGGCCAAAACAGGA
AACATCGTTACCAACTAATTTGATCACCTTTAAAGAACTGGATTTACGCGGTTCCCGTACCAGTGCCGGTGAATTTGAAG
AAGCTCTGCAAATGTTATCAACGCTGAATATTAATCCGGCAGATATCGTGAGTAAAGTCGTGACAATTGATGAAGTTCCT
GAGGCTGTTAAAGAGTTAGATCAATTCCCGGAACGCCATCTGAAAATTAACGTGAAATTCATTTAA

Upstream 100 bases:

>100_bases
AGCGGTAAATATGATTACGCATTCTATCTGGCAGGTGCCATAGCAATTGTTTCAAGTCTGCTGGTATTCGTATTTGTTAA
AAATAAAGGATTGAAAGTCA

Downstream 100 bases:

>100_bases
TTAGCAAAAATCAAAACGCGTAGTGAATTCACTACGCGTTTTTTTAGCGCAGAAAAGGTTAATAACCAATTATTTCTCGC
ACATACTTCAAATGTAGCAG

Product: L-threonine 3-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVLETGKGMPEDIKQ
GDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAMQEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQ
LKAGEHVVIIGAGAIGLMAALSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASG
ANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVP
EAVKELDQFPERHLKINVKFI

Sequences:

>Translated_341_residues
MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVLETGKGMPEDIKQ
GDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAMQEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQ
LKAGEHVVIIGAGAIGLMAALSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASG
ANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVP
EAVKELDQFPERHLKINVKFI
>Mature_341_residues
MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVLETGKGMPEDIKQ
GDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAMQEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQ
LKAGEHVVIIGAGAIGLMAALSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASG
ANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVP
EAVKELDQFPERHLKINVKFI

Specific function: Not Known; Probable Catabolic Enzyme. [C]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=333, Percent_Identity=27.6276276276276, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI71565154, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1787863, Length=340, Percent_Identity=34.1176470588235, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI226510992, Length=285, Percent_Identity=36.4912280701754, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=28.955223880597, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790045, Length=323, Percent_Identity=31.5789473684211, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788407, Length=314, Percent_Identity=27.7070063694268, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790718, Length=320, Percent_Identity=25.3125, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788075, Length=322, Percent_Identity=25.1552795031056, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081918, Length=287, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082125, Length=336, Percent_Identity=26.7857142857143, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786825, Length=228, Percent_Identity=29.3859649122807, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=23.9782016348774, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787572, Length=228, Percent_Identity=27.1929824561404, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=326, Percent_Identity=29.4478527607362, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17562878, Length=324, Percent_Identity=29.0123456790123, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71988145, Length=311, Percent_Identity=24.4372990353698, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562584, Length=298, Percent_Identity=24.8322147651007, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=343, Percent_Identity=30.9037900874636, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322619, Length=331, Percent_Identity=29.3051359516616, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319955, Length=331, Percent_Identity=29.0030211480363, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319258, Length=376, Percent_Identity=28.7234042553192, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319257, Length=351, Percent_Identity=26.7806267806268, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=325, Percent_Identity=25.8461538461538, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=285, Percent_Identity=25.9649122807018, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=264, Percent_Identity=28.7878787878788, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=336, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=267, Percent_Identity=27.3408239700375, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737897, Length=324, Percent_Identity=33.0246913580247, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137530, Length=322, Percent_Identity=31.9875776397516, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17737895, Length=255, Percent_Identity=27.0588235294118, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221457811, Length=178, Percent_Identity=29.2134831460674, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45551930, Length=178, Percent_Identity=29.2134831460674, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550770, Length=178, Percent_Identity=29.2134831460674, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 36810; Mature: 36810

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIG
CCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECHHHHC
HEVTGIVLETGKGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAM
CCCEEEEEECCCCCCHHHHCCCEEEECCEEEECCEEECCCCCCCCCCCCEEEEEEECHHH
QEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKAGEHVVIIGAGAIGLMAA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
LSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCHHHEEEEECCC
ANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLS
CCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
TLNINPADIVSKVVTIDEVPEAVKELDQFPERHLKINVKFI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIG
CCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECHHHHC
HEVTGIVLETGKGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAM
CCCEEEEEECCCCCCHHHHCCCEEEECCEEEECCEEECCCCCCCCCCCCEEEEEEECHHH
QEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKAGEHVVIIGAGAIGLMAA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
LSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCHHHEEEEECCC
ANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLS
CCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
TLNINPADIVSKVVTIDEVPEAVKELDQFPERHLKINVKFI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]