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The map label for this gene is sbmA [H]

Identifier: 218549961

GI number: 218549961

Start: 2715309

End: 2716529

Strand: Reverse

Name: sbmA [H]

Synonym: EFER_2646

Alternate gene names: 218549961

Gene position: 2716529-2715309 (Counterclockwise)

Preceding gene: 218549963

Following gene: 218549960

Centisome position: 59.2

GC content: 48.81

Gene sequence:

>1221_bases
ATGTTTAAGTCTTTTTTCCCAAAGCCTGGGAAATTTTTTCTTTCGGCATTGATATGGGCACTGTTTGCCGTGCTTTTCTG
GCAGGCTGGCGGTGGCGCCTGGATCGCCAGTCTTACGGGAGCCTCAGATAAATTGCCGATTAGCGCCGCCCGTTTCTGGT
CGCTGAATTTTGCTATTTTCTATGCTTATTACATTGTTTGTGTCGGCCTGTTTGCTCTGTTCTGGTTTATCTATAGCCCG
CATCGCTGGCAATACTGGTCGATTCTTGGAACAGCGCTGATTATTTTTGTCACCTGGTTTTTGGTGGAAGTCGGCGTTGC
CATTAACGCATGGTATGCGCCGTTCTATGACTTAATCCAGCAGGCACTTAGCGCACCGCATAAAGTTAAGATTGAACAGT
TTTACCATGAGATAGGCGTATTTCTCGGCATTGCGTTTATCGCGGTAGTGGTGAGTGTGCTGAACAACTTCTTCGTAAGC
CACTATGTTTTTCGCTGGCGTACTGCGATGAACGAATATTACATGGCGAACTGGCAGCAGCTGCGTCATATTGAAGGGGC
CGCGCAACGTGTGCAGGAAGATACCATGCGTTTCGCCTCCACGGTGGAAAGCATGGGCGTGAGTTTACTCAATGCTGTAA
TGACGCTGATCGCCTTTTTGCCGGTTCTGGTGGCACTCTCCGTGCATGTTCCTGAGCTACCGATTGTCGGGCATATTCCG
TATGGTCTGGTGATTGCCGCCATTGTATGGTCTATTGTCGGCACTGGCATTTTGGCAATTGTCGGTATCAAACTTCCTGG
GCTGGAGTTTAACAATCAGCGTGTCGAAGCGGCCTACCGTAAAGAACTGGTATTTGGTGAGGATGATCCTTCTCGCGCCA
CACCACCGACAGTGCGTGAGCTGTTTAGCGCGGTGCGGAAAAACTATTTTCGCCTCTATTTTCACTATATGTACTTCAAT
ATTGCACGAATTTTGTATCTGCAACTCGATAATGTTTTCGGCGTGTTCTTGCTGTTTCCGTCGATTGTTTCTGCCACCAT
TACGCTGGGCTTACTGACGCAAATTACGAATATTTTCGACCGGGTGCGTGGGGCTTTCCAGTATCTGATCACCTCGTGGA
CGACACTGGTGGAATTGATGTCTATCTATAAGCGTCTGCGCAGTTTTGAGCGAGAGCTGGATGGTCAGGAGATTCAGGAG
GTCACGCACACCCTGAGCTAA

Upstream 100 bases:

>100_bases
TCTATTTCGGTCACAATGGGCGGTTTCGCCGGGGCGTTGCAGGCGTGCCGGTTAACACACAGCAGCTTTTAAAACGATAA
GAAGTGAGCAGGAGATCAGC

Downstream 100 bases:

>100_bases
AAGGAAATGTAATGTTGAGGGCAATACGTTTTTCTTCTTATTCACTGCTTGCTGTACTGGTGTTGGCGGGGTGTAGTAGT
AACACCCCGCAGCCAGCTAA

Product: transport protein

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFKSFFPKPGKFFLSALIWALFAVLFWQAGGGAWIASLTGASDKLPISAARFWSLNFAIFYAYYIVCVGLFALFWFIYSP
HRWQYWSILGTALIIFVTWFLVEVGVAINAWYAPFYDLIQQALSAPHKVKIEQFYHEIGVFLGIAFIAVVVSVLNNFFVS
HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTVESMGVSLLNAVMTLIAFLPVLVALSVHVPELPIVGHIP
YGLVIAAIVWSIVGTGILAIVGIKLPGLEFNNQRVEAAYRKELVFGEDDPSRATPPTVRELFSAVRKNYFRLYFHYMYFN
IARILYLQLDNVFGVFLLFPSIVSATITLGLLTQITNIFDRVRGAFQYLITSWTTLVELMSIYKRLRSFERELDGQEIQE
VTHTLS

Sequences:

>Translated_406_residues
MFKSFFPKPGKFFLSALIWALFAVLFWQAGGGAWIASLTGASDKLPISAARFWSLNFAIFYAYYIVCVGLFALFWFIYSP
HRWQYWSILGTALIIFVTWFLVEVGVAINAWYAPFYDLIQQALSAPHKVKIEQFYHEIGVFLGIAFIAVVVSVLNNFFVS
HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTVESMGVSLLNAVMTLIAFLPVLVALSVHVPELPIVGHIP
YGLVIAAIVWSIVGTGILAIVGIKLPGLEFNNQRVEAAYRKELVFGEDDPSRATPPTVRELFSAVRKNYFRLYFHYMYFN
IARILYLQLDNVFGVFLLFPSIVSATITLGLLTQITNIFDRVRGAFQYLITSWTTLVELMSIYKRLRSFERELDGQEIQE
VTHTLS
>Mature_406_residues
MFKSFFPKPGKFFLSALIWALFAVLFWQAGGGAWIASLTGASDKLPISAARFWSLNFAIFYAYYIVCVGLFALFWFIYSP
HRWQYWSILGTALIIFVTWFLVEVGVAINAWYAPFYDLIQQALSAPHKVKIEQFYHEIGVFLGIAFIAVVVSVLNNFFVS
HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTVESMGVSLLNAVMTLIAFLPVLVALSVHVPELPIVGHIP
YGLVIAAIVWSIVGTGILAIVGIKLPGLEFNNQRVEAAYRKELVFGEDDPSRATPPTVRELFSAVRKNYFRLYFHYMYFN
IARILYLQLDNVFGVFLLFPSIVSATITLGLLTQITNIFDRVRGAFQYLITSWTTLVELMSIYKRLRSFERELDGQEIQE
VTHTLS

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=406, Percent_Identity=87.6847290640394, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI1787772, Length=314, Percent_Identity=24.5222929936306, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 46475; Mature: 46475

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]