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The map label for this gene is proY [H]

Identifier: 218549938

GI number: 218549938

Start: 2692888

End: 2694261

Strand: Reverse

Name: proY [H]

Synonym: EFER_2623

Alternate gene names: 218549938

Gene position: 2694261-2692888 (Counterclockwise)

Preceding gene: 218549939

Following gene: 218549937

Centisome position: 58.71

GC content: 51.38

Gene sequence:

>1374_bases
ATGGAAAGTAACAACAAGCTAAAGCGTGGGCTCAGTACCCGGCATATTCGCTTTATGGCACTGGGTTCAGCAATCGGCAC
CGGTTTGTTTTACGGTTCAGCCGATGCCATCAAAATGGCCGGTCCCAGCGTACTACTGGCTTATATTATCGGCGGGGTAG
CTGCATATATTATTATGCGTGCACTGGGAGAAATGTCTGTTCACAACCCGGCTGCAAGCTCCTTCTCTCGTTATGCACAG
GAAAACCTCGGCCCGCTGGCGGGTTATATCACTGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGT
GACCGCGTTCGGTATCTATATGGGCGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCA
TTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTTGGTGAGCTTGAGTTCTGGTTCTCGTTCTTTAAAGTCGCCACCATC
ATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATCAATAATCT
TTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTGATGTCGCTGCAAATGGTAATGTTTGCTTACGGCG
GGATCGAAATTATCGGTATTACCGCCGGGGAAGCGAAAGATCCTGAGAAATCGATTCCGCGTGCGATTAACTCCGTGCCG
ATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTATCCGTGGAATCAGGTTGGTACTGCCGG
TAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCTTAACTTTGTTGTGCTGACTGCTT
CGCTGTCGGCAATTAACAGTGACGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGTAGCGCGCCGAAA
ATTTTCAGCAAAACCTCGCGTCGCGGTATTCCGTGGGTCACGGTGCTGGTGATGACTACCGCGCTATTGTTTGCGGTATA
TCTGAACTACATCATGCCGGAAAATGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTATGGATTA
TGATCCTGCTGTCGCAAATTGCTTTCCGTCGCCGTTTGCCACCAGAAGAAGTGAAGGCACTGAAATTTAAAGTACCAGGT
GGGGTAGCAACGACCATCGGCGGTTTGATTTTCCTGCTCTTTATTATCGGATTGATTGGTTATCATCCGGACACACGTAT
CTCGCTGTACGTTGGTTTCGCGTGGATTGTTCTGCTGTTGATTGGCTGGATGATTAAACGCCGCCACGATCGTCAGCTGG
CTGAAAACCAGTAA

Upstream 100 bases:

>100_bases
CAGGTGACCTCCAGCGCTCACTAAATCACTGACAATGTGTTTTTGACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTG
TGTTGATGGGTTAGGAATCG

Downstream 100 bases:

>100_bases
TCCTTGCGTTCTGTAAGCCGGATCAAGGCAAGCCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCC
AAATATCCTTCAGATGATGA

Product: proline-specific permease

Products: Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MESNNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGINNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVLLLIGWMIKRRHDRQLAENQ

Sequences:

>Translated_457_residues
MESNNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGINNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVLLLIGWMIKRRHDRQLAENQ
>Mature_457_residues
MESNNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGINNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVLLLIGWMIKRRHDRQLAENQ

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of proline [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI181337167, Length=338, Percent_Identity=25.1479289940828, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI110347453, Length=351, Percent_Identity=24.2165242165242, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI258645169, Length=432, Percent_Identity=22.4537037037037, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI258614003, Length=430, Percent_Identity=22.093023255814, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI258614005, Length=430, Percent_Identity=22.093023255814, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4507047, Length=364, Percent_Identity=23.9010989010989, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786602, Length=457, Percent_Identity=98.9059080962801, Blast_Score=903, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=453, Percent_Identity=45.0331125827815, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786302, Length=453, Percent_Identity=43.046357615894, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI48994972, Length=437, Percent_Identity=45.766590389016, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790653, Length=460, Percent_Identity=40.4347826086956, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI87081915, Length=400, Percent_Identity=38.25, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87081708, Length=462, Percent_Identity=35.9307359307359, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1788480, Length=406, Percent_Identity=37.192118226601, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789017, Length=428, Percent_Identity=33.8785046728972, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI87081869, Length=176, Percent_Identity=26.7045454545455, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082023, Length=287, Percent_Identity=24.7386759581882, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786694, Length=435, Percent_Identity=23.9080459770115, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=425, Percent_Identity=23.0588235294118, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531343, Length=360, Percent_Identity=24.1666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17533459, Length=401, Percent_Identity=22.9426433915212, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540018, Length=459, Percent_Identity=22.6579520697168, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324061, Length=462, Percent_Identity=32.9004329004329, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6320772, Length=467, Percent_Identity=32.5481798715203, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324990, Length=440, Percent_Identity=32.0454545454545, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324924, Length=407, Percent_Identity=31.6953316953317, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322892, Length=475, Percent_Identity=30.3157894736842, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324059, Length=410, Percent_Identity=33.1707317073171, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319824, Length=432, Percent_Identity=28.0092592592593, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319542, Length=430, Percent_Identity=27.6744186046512, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320251, Length=437, Percent_Identity=27.4599542334096, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321629, Length=479, Percent_Identity=27.1398747390397, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324553, Length=397, Percent_Identity=31.9899244332494, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320717, Length=403, Percent_Identity=28.0397022332506, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319543, Length=402, Percent_Identity=28.1094527363184, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321053, Length=390, Percent_Identity=26.1538461538462, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319608, Length=454, Percent_Identity=25.3303964757709, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322967, Length=395, Percent_Identity=25.3164556962025, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324981, Length=397, Percent_Identity=25.4408060453401, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320364, Length=505, Percent_Identity=24.950495049505, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI221512776, Length=413, Percent_Identity=21.5496368038741, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24666159, Length=413, Percent_Identity=21.5496368038741, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161077963, Length=333, Percent_Identity=25.5255255255255, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24667468, Length=341, Percent_Identity=22.2873900293255, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50145; Mature: 50145

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESNNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMR
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGINNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVLLLIGWMIKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MESNNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMR
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGINNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVLLLIGWMIKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]