Click here to switch to the map view.

The map label for this gene is ybfF [H]

Identifier: 218549745

GI number: 218549745

Start: 2486467

End: 2487231

Strand: Direct

Name: ybfF [H]

Synonym: EFER_2422

Alternate gene names: 218549745

Gene position: 2486467-2487231 (Clockwise)

Preceding gene: 218549742

Following gene: 218549746

Centisome position: 54.19

GC content: 51.37

Gene sequence:

>765_bases
ATGAAATTGAATATCCGCACGCAAACTGCACAAAACCAGCACAATAATTCTCCCATCGTCCTCGTACACGGCCTGTTTGG
CAGCCTCGACAACCTTGGCGTACTGGCTCGCGATCTGGTAAACGATCACAATATCATCCAGGTTGATATGCGTAACCACG
GTCTTTCACCGAGAGATCCGGTGATGAGTTACCCGGCGATGGCCCAGGATCTTGTTGACACTCTGGATGCACAGCAGATC
GATAAGGCAACATTTATTGGACACTCAATGGGCGGTAAAGCGGTGATGGCACTGACTGCACTGGCCCCCGATCGCATCGA
TAAACTGGTGGCGATTGATATCGCGCCGGTCGACTATCACGTACGCCGTCATGATGAAATTTTCGCGGCTATCAACGCAG
TCAGTGAATCCGACGCGCAAACTCGCCAGCAAGCAGCGGCAGTAATGCGCCAGCATCTTAATGAAGAAGGGGTGATTCAG
TTTCTGTTGAAATCTTTTGTTGACGGGGAGTGGCGCTTTAACGTGCCGGTATTGTGGGATCAGTATCCGCATATTGTGGG
GTGGGAGAAAATCCCGGCATGGGATCACCCTGCCCTGTTTATCCCTGGTGGCAATTCTCCGTATGTTAGCGAGCAGTATC
GTGATGATTTACTGACACAATTTCCACAGGCACGAGCGCATGTGATTGCTGGCGCGGGTCACTGGGTCCATGCTGAAAAA
CCGGATGCGGTATTACGCGCTATCCGTCGCTATCTCAATGATTAA

Upstream 100 bases:

>100_bases
CTTGCAGAAAAAATCGGAGTAACCCGTATTTGTTGCAGCCCTGTGGCACAATGGTCAACATAATACCCTAACCCGACAAC
GCGAAAAAAAGAACTTTGCC

Downstream 100 bases:

>100_bases
CGTAACTGATTAAAAACCCAGCGTCCGCGTCGCTTTCCACGCGTGGGCGTTGGCGTGACTCATCGCGCTGATGTATTATG
GCGCGCTATCCATCCGGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKLNIRTQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMSYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAVMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLTQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND

Sequences:

>Translated_254_residues
MKLNIRTQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMSYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAVMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLTQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND
>Mature_254_residues
MKLNIRTQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMSYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAVMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLTQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=34.9593495934959, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=34.9593495934959, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI223941839, Length=239, Percent_Identity=30.5439330543933, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786902, Length=254, Percent_Identity=98.0314960629921, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17508535, Length=254, Percent_Identity=33.8582677165354, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17507063, Length=256, Percent_Identity=27.34375, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321468, Length=261, Percent_Identity=31.0344827586207, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640348, Length=272, Percent_Identity=27.9411764705882, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646055, Length=274, Percent_Identity=24.8175182481752, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28467; Mature: 28467

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNIRTQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDP
CEEEEECCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VMSYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEECCCCHH
VRRHDEIFAAINAVSESDAQTRQQAAAVMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLTQFPQARAHVIAGAGHWVHAEK
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDAVLRAIRRYLND
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKLNIRTQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDP
CEEEEECCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VMSYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEECCCCHH
VRRHDEIFAAINAVSESDAQTRQQAAAVMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLTQFPQARAHVIAGAGHWVHAEK
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDAVLRAIRRYLND
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]