Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 218549706

GI number: 218549706

Start: 2442447

End: 2443370

Strand: Reverse

Name: gcvA [H]

Synonym: EFER_2383

Alternate gene names: 218549706

Gene position: 2443370-2442447 (Counterclockwise)

Preceding gene: 218549707

Following gene: 218549696

Centisome position: 53.25

GC content: 43.61

Gene sequence:

>924_bases
ATGACTTTCAGAGATTGGCATATGAGAGGAAAAATACCGAAAACAGAACTTCTGATCGCTTTTGAGGCGGTGGCCCGCTA
TGAAAGCTATACCCGGGCGGCAGAAGATCTGGCATTGACACAGAGTGCCGTGTTCCGTCAGATTACTGCTCTGGAAGAGT
TTTTGTGCACACCACTGTTTGACCATGCGAAAAAGCGTATTACCCTGAATTCTGCTGGAAAATATTATCTCGGTATTATC
AAAGAAACACTTCATAAGATGGAGCGCGATACGCATGCCATTATGAGTTGGCAACCGAAAGTGCAGGTAATAGAACTGGC
AGTCAATCCAACTTTTAGTACCCACTGGTTAATACCCAGTCTGCGGGAGTTCAGCGAACTTAACCCGGATATTATTGTTA
ATATATATTCACTTTCGAGTGTGGATGATTTTCTTCATCGGGAATATGACGCGGCAATTATGCGTGAAGATTTTTGTACC
CACTGGGCAGAAGTCGAATTTCTTTTTGAAGAGGAAATTCTACCTGTATGCAGCAGTACACTACTTGCGCCTGAAGATCA
AAAATTATCAGTAAAACAGTTGGTTAGTGATATACAGTTGTTGCATCAAAGCACTCGTATGAATGCATGGCAGGAGTGGT
TTGCGCTTTCAGGTATTATGCATTCAGGTATTAATAAAGGCCCACGTTATGATTTATTATCCATGTTGATTGCGGCCGTG
CGTTCCAATCTTGGCGTGGCGTTGCTTCCCCGCTTTGCAATTCAGCGAGATCTTGATAATGGCGAAATGATTATTCCCTG
TGATGTACCGATTCGCACCGGAAATCGATTTGTTTTGACGTGGCGGGAAGAGAAAAATAAATCCCGCCATTTGCAGATTT
TTCGTGACTGGCTGCGGGAAAAGTCGCAAACCACCATGCTTTGA

Upstream 100 bases:

>100_bases
ATTCTTTTGCGAGTTTGTTTTAATTAAACCATCGGGCTGGTTGGTAGCTTTAAAATAACACGCGGTGGTGAAGGGATATC
ATCCGGCTTGCAGAAAATAA

Downstream 100 bases:

>100_bases
GCATCTTTTATTTGCCTGCCAGACAACTGTTGCAATGGCATGACTGCTCAAACTGACTAAAGAAGTTATTTCCTTTGTCA
TCCACCAACAGAAAAGCAGG

Product: LysR-like transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTFRDWHMRGKIPKTELLIAFEAVARYESYTRAAEDLALTQSAVFRQITALEEFLCTPLFDHAKKRITLNSAGKYYLGII
KETLHKMERDTHAIMSWQPKVQVIELAVNPTFSTHWLIPSLREFSELNPDIIVNIYSLSSVDDFLHREYDAAIMREDFCT
HWAEVEFLFEEEILPVCSSTLLAPEDQKLSVKQLVSDIQLLHQSTRMNAWQEWFALSGIMHSGINKGPRYDLLSMLIAAV
RSNLGVALLPRFAIQRDLDNGEMIIPCDVPIRTGNRFVLTWREEKNKSRHLQIFRDWLREKSQTTML

Sequences:

>Translated_307_residues
MTFRDWHMRGKIPKTELLIAFEAVARYESYTRAAEDLALTQSAVFRQITALEEFLCTPLFDHAKKRITLNSAGKYYLGII
KETLHKMERDTHAIMSWQPKVQVIELAVNPTFSTHWLIPSLREFSELNPDIIVNIYSLSSVDDFLHREYDAAIMREDFCT
HWAEVEFLFEEEILPVCSSTLLAPEDQKLSVKQLVSDIQLLHQSTRMNAWQEWFALSGIMHSGINKGPRYDLLSMLIAAV
RSNLGVALLPRFAIQRDLDNGEMIIPCDVPIRTGNRFVLTWREEKNKSRHLQIFRDWLREKSQTTML
>Mature_306_residues
TFRDWHMRGKIPKTELLIAFEAVARYESYTRAAEDLALTQSAVFRQITALEEFLCTPLFDHAKKRITLNSAGKYYLGIIK
ETLHKMERDTHAIMSWQPKVQVIELAVNPTFSTHWLIPSLREFSELNPDIIVNIYSLSSVDDFLHREYDAAIMREDFCTH
WAEVEFLFEEEILPVCSSTLLAPEDQKLSVKQLVSDIQLLHQSTRMNAWQEWFALSGIMHSGINKGPRYDLLSMLIAAVR
SNLGVALLPRFAIQRDLDNGEMIIPCDVPIRTGNRFVLTWREEKNKSRHLQIFRDWLREKSQTTML

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=30.5084745762712, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=27.2108843537415, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=25.9649122807018, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=22.9235880398671, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35702; Mature: 35570

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRDWHMRGKIPKTELLIAFEAVARYESYTRAAEDLALTQSAVFRQITALEEFLCTPLF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
DHAKKRITLNSAGKYYLGIIKETLHKMERDTHAIMSWQPKVQVIELAVNPTFSTHWLIPS
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
LREFSELNPDIIVNIYSLSSVDDFLHREYDAAIMREDFCTHWAEVEFLFEEEILPVCSST
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAPEDQKLSVKQLVSDIQLLHQSTRMNAWQEWFALSGIMHSGINKGPRYDLLSMLIAAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RSNLGVALLPRFAIQRDLDNGEMIIPCDVPIRTGNRFVLTWREEKNKSRHLQIFRDWLRE
HHCCCHHHHHHHHHHHCCCCCCEEEECCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHH
KSQTTML
HHCCCCC
>Mature Secondary Structure 
TFRDWHMRGKIPKTELLIAFEAVARYESYTRAAEDLALTQSAVFRQITALEEFLCTPLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
DHAKKRITLNSAGKYYLGIIKETLHKMERDTHAIMSWQPKVQVIELAVNPTFSTHWLIPS
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
LREFSELNPDIIVNIYSLSSVDDFLHREYDAAIMREDFCTHWAEVEFLFEEEILPVCSST
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAPEDQKLSVKQLVSDIQLLHQSTRMNAWQEWFALSGIMHSGINKGPRYDLLSMLIAAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RSNLGVALLPRFAIQRDLDNGEMIIPCDVPIRTGNRFVLTWREEKNKSRHLQIFRDWLRE
HHCCCHHHHHHHHHHHCCCCCCEEEECCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHH
KSQTTML
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]