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The map label for this gene is bioF [H]

Identifier: 218549661

GI number: 218549661

Start: 2393065

End: 2394219

Strand: Reverse

Name: bioF [H]

Synonym: EFER_2337

Alternate gene names: 218549661

Gene position: 2394219-2393065 (Counterclockwise)

Preceding gene: 218549662

Following gene: 218549659

Centisome position: 52.18

GC content: 58.35

Gene sequence:

>1155_bases
ATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTACTGCCGATGCCCTGCGTCGCCGTTATCCAGTGGC
GCAAGGGGCCGGTCGCTGGCTGGTGGCGGATGAATGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCC
ATCATCCGGACATCATTCGTGCATGGAAGCAGGGTGCGGAGCAATTTGGCGTCGGTAGCGGCGGTTCCGGTCACGTCAGC
GGTTATAGCGTGGCGCATCAGGCGCTGGAAGAAGAACTGGCCGAGTGGCTGGGCTATTCGCGGGCACTGCTGTTTATCTC
TGGTTTTGCCGCTAACCAGGCAGTCATTACCGCGATGATGGCAAAAGAGGACCGCATTGTTGCCGACCGGCTGAGCCATG
CCTCCTTGCTGGAAGCTGCCAGTTTAAGCCCCGCGCAGCTTCGCCGTTTTACTCATAACGATGTCGCTCATCTGGCGCGA
CTGCTTGCTTCCCCCTGTCCGGGGCAGCAGCTGGTGGTGACAGAAGGTGTATTCAGCATGGACGGCGATAGGGCACCACT
GGCGGAAATCCAGCAGGTAACGCAACAGCACAATGGCTGGTTGGTGGTCGACGATGCCCACGGCACGGGCGTGATCGGTG
AACAGGGGCGTGGTAGCTGCTGGCAACAGCAGGTGAAACCGGAACTGCTGGTGGTCACTTTTGGCAAAGGATTTGGCGTC
AGCGGGGCAGCGGTGCTTTGCTCCAATACAGTGGCGGATTATCTGCTGCAATTCGCCCGCCATCTTATCTACAGCACCAG
TATGCCGCCCGCTCAGGCGCAGGCATTACGCGCGTCGCTGGCGGTCATTCGCGGTGAAGAGGGGGACGCTCGGCGCGAAA
AACTGGCGGTACTCATTACGCGTTTTCGTGCCGGATTGCAGGGTTTGCCGTTTACCCTTGCAGATTCACGTAGCGCCATT
CAGCCATTAATCGTTGGCGATAACGCTCGTGCATTACATCTGGCAGAAAAACTGCGTCAGCAAGGCTGTTGGGTCACGGC
AATTCGCCCACCAACCGTACCCGCAGGCACTGCGCGATTGCGCCTGACCTTAACCGCCGCACATGAAACGCAGGATATCG
ACCTGCTGCTGGAGGTGCTGCATGGCAACGGTTAA

Upstream 100 bases:

>100_bases
AGCAAACCGCCGTGCTGGCGGGCGATAACGAACAACAGCAACGTCTGGAGCAGGCACTAATGACCCCGGACACCGACGAA
TATTACAACGCGGCAGCACT

Downstream 100 bases:

>100_bases
TAAACAAGCCATTGCCGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACACGCAGATCTACAGCGTCAGAGTGCTG
ACGCCTTACTGGCAATGCTC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADECQYLNFSSNDYLGLSHHPDIIRAWKQGAEQFGVGSGGSGHVS
GYSVAHQALEEELAEWLGYSRALLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLAR
LLASPCPGQQLVVTEGVFSMDGDRAPLAEIQQVTQQHNGWLVVDDAHGTGVIGEQGRGSCWQQQVKPELLVVTFGKGFGV
SGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRGEEGDARREKLAVLITRFRAGLQGLPFTLADSRSAI
QPLIVGDNARALHLAEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHETQDIDLLLEVLHGNG

Sequences:

>Translated_384_residues
MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADECQYLNFSSNDYLGLSHHPDIIRAWKQGAEQFGVGSGGSGHVS
GYSVAHQALEEELAEWLGYSRALLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLAR
LLASPCPGQQLVVTEGVFSMDGDRAPLAEIQQVTQQHNGWLVVDDAHGTGVIGEQGRGSCWQQQVKPELLVVTFGKGFGV
SGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRGEEGDARREKLAVLITRFRAGLQGLPFTLADSRSAI
QPLIVGDNARALHLAEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHETQDIDLLLEVLHGNG
>Mature_383_residues
SWQEKINAALDARRTADALRRRYPVAQGAGRWLVADECQYLNFSSNDYLGLSHHPDIIRAWKQGAEQFGVGSGGSGHVSG
YSVAHQALEEELAEWLGYSRALLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLARL
LASPCPGQQLVVTEGVFSMDGDRAPLAEIQQVTQQHNGWLVVDDAHGTGVIGEQGRGSCWQQQVKPELLVVTFGKGFGVS
GAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRGEEGDARREKLAVLITRFRAGLQGLPFTLADSRSAIQ
PLIVGDNARALHLAEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHETQDIDLLLEVLHGNG

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI284448556, Length=343, Percent_Identity=32.9446064139942, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI7657118, Length=343, Percent_Identity=32.9446064139942, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI83977442, Length=375, Percent_Identity=34.1333333333333, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI83977440, Length=375, Percent_Identity=34.1333333333333, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI83977444, Length=372, Percent_Identity=33.6021505376344, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4502025, Length=389, Percent_Identity=31.3624678663239, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI40316939, Length=389, Percent_Identity=31.3624678663239, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI119220554, Length=358, Percent_Identity=30.1675977653631, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4758668, Length=356, Percent_Identity=28.6516853932584, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI5454084, Length=192, Percent_Identity=28.125, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786993, Length=384, Percent_Identity=92.7083333333333, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1790046, Length=352, Percent_Identity=34.9431818181818, Blast_Score=205, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71994529, Length=358, Percent_Identity=32.122905027933, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32566772, Length=381, Percent_Identity=29.3963254593176, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17560914, Length=361, Percent_Identity=27.9778393351801, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560912, Length=361, Percent_Identity=27.9778393351801, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71982617, Length=294, Percent_Identity=25.8503401360544, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982625, Length=294, Percent_Identity=25.8503401360544, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320438, Length=409, Percent_Identity=26.8948655256724, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320267, Length=356, Percent_Identity=28.6516853932584, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=187, Percent_Identity=31.5508021390374, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662918, Length=339, Percent_Identity=32.1533923303835, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137420, Length=341, Percent_Identity=31.0850439882698, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136286, Length=367, Percent_Identity=28.0653950953678, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24653280, Length=269, Percent_Identity=24.5353159851301, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653276, Length=269, Percent_Identity=24.5353159851301, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653278, Length=269, Percent_Identity=24.5353159851301, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 41575; Mature: 41444

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADECQYLNFSSNDYLGLSHHPDIIR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCCCCEECCCCCHHHHH
AWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRALLFISGFAANQAVITAMM
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHH
AKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLARLLASPCPGQQLVVTEGVFSM
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
DGDRAPLAEIQQVTQQHNGWLVVDDAHGTGVIGEQGRGSCWQQQVKPELLVVTFGKGFGV
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCC
SGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRGEEGDARREKLAVLIT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
RFRAGLQGLPFTLADSRSAIQPLIVGDNARALHLAEKLRQQGCWVTAIRPPTVPAGTARL
HHHHCCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
RLTLTAAHETQDIDLLLEVLHGNG
EEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SWQEKINAALDARRTADALRRRYPVAQGAGRWLVADECQYLNFSSNDYLGLSHHPDIIR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCCCCEECCCCCHHHHH
AWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRALLFISGFAANQAVITAMM
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHH
AKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLARLLASPCPGQQLVVTEGVFSM
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
DGDRAPLAEIQQVTQQHNGWLVVDDAHGTGVIGEQGRGSCWQQQVKPELLVVTFGKGFGV
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCC
SGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRGEEGDARREKLAVLIT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
RFRAGLQGLPFTLADSRSAIQPLIVGDNARALHLAEKLRQQGCWVTAIRPPTVPAGTARL
HHHHCCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
RLTLTAAHETQDIDLLLEVLHGNG
EEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA