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The map label for this gene is moaA [H]

Identifier: 218549655

GI number: 218549655

Start: 2386721

End: 2387710

Strand: Reverse

Name: moaA [H]

Synonym: EFER_2330

Alternate gene names: 218549655

Gene position: 2387710-2386721 (Counterclockwise)

Preceding gene: 218549657

Following gene: 218549654

Centisome position: 52.03

GC content: 53.03

Gene sequence:

>990_bases
ATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTCCG
TTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCC
GGGTTACGCGCGCCTTCGCCAGTCTGGGTACCGAAAAAGTCCGTCTGACGGGCGGAGAGCCGTCTTTGCGTCGTGACTTT
ACCGATATCATCGCCGCTGTGCGAGAAAACGACGCTATTCGCCAGATAGCGGTCACCACCAATGGTTACCGTCTGGAACG
CGACGTGGCGAACTGGCGTGATGCTGGTCTTACTGGTATCAACGTGAGTGTCGATAGCCTCGACGCTCGCCAGTTTCAGG
CTATTACCGGACAGGATAAATTCAATCAGGTCATGGCAGGGATCGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAA
GTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCA
GTTACGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGGAAGCATCACATCTCTGGTCAGGTTCTAC
GTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGATGGTCCCGCGCAAGTCTTTTGTCATCCG
GATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGTGCCACTTGCAACCGCCTGCGCGTTTCCTC
CATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACTTGCGCGATCTGCTGGAAGACGATACCCAGCAAC
AGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAAAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATT
ACGCAAAACTTATCGTACATTGGCGGCTAA

Upstream 100 bases:

>100_bases
GGTCAACTGATACGGTGCTTTGGCCGTGACAATGCTTACAAAGATTGCCACCAGGGCGAAGGAAGAAATGACTTCGCCTC
CCGTATCTGGAAAGGTGTAC

Downstream 100 bases:

>100_bases
AACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAA
TCGTCGCGGTGAAGAAGACG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFQAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG

Sequences:

>Translated_329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFQAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG
>Mature_328_residues
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFT
DIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFQAITGQDKFNQVMAGIDAAFEAGFEKVKV
NTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHPD
YAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGIT
QNLSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=310, Percent_Identity=39.3548387096774, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=39.3548387096774, Blast_Score=221, Evalue=9e-58,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=99.0881458966565, Blast_Score=678, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566331, Length=313, Percent_Identity=36.1022364217252, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=36.3636363636364, Blast_Score=200, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=35.1791530944625, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI28574490, Length=315, Percent_Identity=38.0952380952381, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI28574488, Length=315, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37296; Mature: 37165

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFQAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCCEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFQAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCCEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA