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The map label for this gene is insG [H]

Identifier: 218549587

GI number: 218549587

Start: 2318576

End: 2319904

Strand: Reverse

Name: insG [H]

Synonym: EFER_2260

Alternate gene names: 218549587

Gene position: 2319904-2318576 (Counterclockwise)

Preceding gene: 218549597

Following gene: 218549585

Centisome position: 50.56

GC content: 53.95

Gene sequence:

>1329_bases
ATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTATGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCT
CGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCTGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGA
TGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTGTGAATCGCCTGGACATCATGCTG
CCGGGCAATCGCCCTTTCGTTGCCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGGGAAGTGAGGCTGTCCGCCGCGT
GTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCAATGGTGCGGCCTGACCCTGCTGGCCATCGATG
GTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACATGCCGGGAACCCGGCGCTC
TACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGCCTTCGGCACGATGAAGAA
CAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAATGGATAAAGGTTATTACT
CACTGGGACTGTTAAATGCCTGGAGCCTGGCGGGAGAACACCGCCACTGGATGATCCCTCTCAGAAAGGGAGCGCAATAT
GAAGAGATCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCACAGGCACGAAAAAAGTGGCCGGG
ACTGGGAAATGAGGTGACAGCCCGCCTGCTGACTGTGACGCGCAAAGGAAAGGTCTGCCATCTGCTGACGTCGATGACGG
ACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATCGAACTGGGATACAGGGAGATA
AAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAGCCGGAGCTTGTGGAGCAAGAGCTGTGGGGTGTCTT
ACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAACATCAGAAAGGTTACTGGCCGAATCAACTGAGTT
TCTCAGAATCATGCGGAATGGTGATGAGAATGCTGATGACATTGCAGGGCGCTTCACCGGGACGTATACCGGAGCTGATG
CGCGATCTTGCAAGTATGGGACAACTTGTGAAATTACCGACAAGAAGGGAAAGGGCCTTCCCGAGAGTGGTAAAGGAGAG
GCCCTGGAAATACCCCACAGCCCCGAAAAAGAGCCAGTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
AGGGCAGCCTGCGCCGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTC
ACTCGTTTCCGGATTTTTTT

Downstream 100 bases:

>100_bases
CTGACTGGCATTACAGCCTGCGCCGCCCTCAGTTTCAGATTGCGCAAATTAGTCTGCGCGGCCCATATAACGGCGTTCTT
CGATATGGATACGAATTTTT

Product: IS4 transposase; KpLE2 phage-like element

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHQKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Sequences:

>Translated_442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHQKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA
>Mature_442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHQKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=442, Percent_Identity=99.0950226244344, Blast_Score=905, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 50364; Mature: 50364

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATP
HHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAA
CCCHHCEEHEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCH
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPG
HHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
CCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEHQKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
PRVVKERPWKYPTAPKKSQSVA
HHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATP
HHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAA
CCCHHCEEHEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQY
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCH
EEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPG
HHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
CCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEHQKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
PRVVKERPWKYPTAPKKSQSVA
HHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]