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The map label for this gene is fruB [H]

Identifier: 218549583

GI number: 218549583

Start: 2314893

End: 2316023

Strand: Reverse

Name: fruB [H]

Synonym: EFER_2256

Alternate gene names: 218549583

Gene position: 2316023-2314893 (Counterclockwise)

Preceding gene: 218549585

Following gene: 218549582

Centisome position: 50.47

GC content: 51.11

Gene sequence:

>1131_bases
ATGTTTCAGTTATCTGTACAAGATATTCATCCGGGCCAACAGGCCGGAAACAAAGAAGATGCCATTCGACACATTGCCGC
TGCGCTGGTACAGGCCGGTAACGTTGCTGAGGGTTACGTCAATGGGATGCTGGCACGCGAACAGCAGACCTCCACGTTCC
TGGGTAATGGTATTGCTATTCCCCATGGCACAACTGACACGCGTGATCAGGTATTGAAAACCGGTGTTCAGGTGTTTCAA
TTCCCGGAAGGTGTTACCTGGGGCGAAGGGCAGACCGCATATGTGGCGATTGGTATAGCGGCAAGTTCTGATGAACATCT
CGGCTTGCTACGTCAGTTAACGCACGTATTGAGTGATGACTCTGTTGCTGGTCAACTTAAATCAGCCACGACAGCGGAAG
AGCTGCGGGCATTGCTGATGGGTGAAAAGCAAAGCGAACAGCTGAAATTAGATAATGACATGCTGATGCTGGATGTTGCT
GCAAATGATCTGGTCACCTTACAGGCGTTGAATGCTGCACGTCTGAAAGAGGCTGGCGCTGTTGATGCCGCGTTCGTTGC
TAAAGCTATCAATGAATCTCCGATGCATCTCGGACAGGGGATTTGGCTCAATGATGGTGTTGAAGGCAACCTGGCAAGCG
CTGTTGCGGTCAGCCGTGCAGCGAATGCATTTGAAGTGGAAGGGGATCCTGCCGCATTACTGCTGACTGTCGCCATGAAT
GACGAACAGCCGTTAGCCGTCCTGAAGCGTGTTGCCGATCTGCTGCTCGATAACAAAGCAGATCGTCTGCTGAAAGCAGA
TTCAGCGACATTGCTGGCGCTGCTGACCAGCGATGATGCGCCGACTGACGACGTGTTAAGTGCGGAATTTGTAGTGCGTA
ATGAACACGGTTTGCACGCCCGTCCTGGCACAATGTTGGTGAACACGATTAAACAATTTAACAGTGAAATCACGGTGACA
AACCTTGATGGCACTGGAAAACCTGCGAACGGAAGGAGCTTGATGAAAGTCGTAGCGTTAGGAGTTAAGAAAGGTCATCG
TCTACGCTTTACCGCACAAGGTGAAGATGCTGAACAGGCGCTGAATGCTATTGGCGATGCAATCGCAGCAGGTCTTGGGG
AGGGTGTGTAA

Upstream 100 bases:

>100_bases
TGAAACGTTTCAGCGCTATCGTATTATCAACCAAGTGATCGAATGGCGCTTTTTTTCTCATGTTGGCTGAATCGTTTCAA
TTCAGCGGGAGAGGAGAACC

Downstream 100 bases:

>100_bases
TGAGCAGACGTGTTGCCACTATCACCCTTAACCCGGCTTATGATCTGGTCGGTTTCTGCCCGGAAATTGAACGTGGTGAA
GTTAATCTGGTGAAAACCAC

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFQLSVQDIHPGQQAGNKEDAIRHIAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAGQLKSATTAEELRALLMGEKQSEQLKLDNDMLMLDVA
ANDLVTLQALNAARLKEAGAVDAAFVAKAINESPMHLGQGIWLNDGVEGNLASAVAVSRAANAFEVEGDPAALLLTVAMN
DEQPLAVLKRVADLLLDNKADRLLKADSATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSEITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAEQALNAIGDAIAAGLGEGV

Sequences:

>Translated_376_residues
MFQLSVQDIHPGQQAGNKEDAIRHIAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAGQLKSATTAEELRALLMGEKQSEQLKLDNDMLMLDVA
ANDLVTLQALNAARLKEAGAVDAAFVAKAINESPMHLGQGIWLNDGVEGNLASAVAVSRAANAFEVEGDPAALLLTVAMN
DEQPLAVLKRVADLLLDNKADRLLKADSATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSEITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAEQALNAIGDAIAAGLGEGV
>Mature_376_residues
MFQLSVQDIHPGQQAGNKEDAIRHIAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAGQLKSATTAEELRALLMGEKQSEQLKLDNDMLMLDVA
ANDLVTLQALNAARLKEAGAVDAAFVAKAINESPMHLGQGIWLNDGVEGNLASAVAVSRAANAFEVEGDPAALLLTVAMN
DEQPLAVLKRVADLLLDNKADRLLKADSATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSEITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAEQALNAIGDAIAAGLGEGV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=375, Percent_Identity=89.6, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=44.8275862068966, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39574; Mature: 39574

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLSVQDIHPGQQAGNKEDAIRHIAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAGQLKSATTAEELRALLMGEKQSEQLKLDNDMLMLDVAANDLVTLQALNAARLKEAGA
HHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCEEEEEHHHHHHHHHCCC
VDAAFVAKAINESPMHLGQGIWLNDGVEGNLASAVAVSRAANAFEVEGDPAALLLTVAMN
HHHHHHHHHHCCCCHHHCCCCEECCCCCCCHHHHHHHHHCCCEEEECCCCEEEEEEEEEC
DEQPLAVLKRVADLLLDNKADRLLKADSATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSEITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAEQA
CCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LNAIGDAIAAGLGEGV
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFQLSVQDIHPGQQAGNKEDAIRHIAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAGQLKSATTAEELRALLMGEKQSEQLKLDNDMLMLDVAANDLVTLQALNAARLKEAGA
HHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCEEEEEHHHHHHHHHCCC
VDAAFVAKAINESPMHLGQGIWLNDGVEGNLASAVAVSRAANAFEVEGDPAALLLTVAMN
HHHHHHHHHHCCCCHHHCCCCEECCCCCCCHHHHHHHHHCCCEEEECCCCEEEEEEEEEC
DEQPLAVLKRVADLLLDNKADRLLKADSATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSEITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAEQA
CCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LNAIGDAIAAGLGEGV
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]