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The map label for this gene is thiM

Identifier: 218549519

GI number: 218549519

Start: 2235619

End: 2236407

Strand: Reverse

Name: thiM

Synonym: EFER_2190

Alternate gene names: 218549519

Gene position: 2236407-2235619 (Counterclockwise)

Preceding gene: 218549522

Following gene: 218549518

Centisome position: 48.74

GC content: 51.84

Gene sequence:

>789_bases
ATGCAGCCTGACCTGCTAAGCCGCAAAACTGCGGCGTTAACATTACAAAAATTTCACACGCTATCGCCACTTACACACTG
CATGACCAATGATGTAGTGCAAACTTTTACTGCCAACACTTTGCTGGCGCTAGGTGCTTCCCCCGCAATGGTGATTGAAA
CAGAAGAAGCCAGCCAGTTTGCTGCTGTTGCCAGTGCATTGTTAATAAATGTTGGCACTCTCACCCAAGCGCGTGCGCAC
GCAATGCGTGCAGCTGTAGTCCATGCAAAACGGGTAAAAACCCCCTGGACGCTGGACCCGGTAGCTGTTGGTTCGCTTGA
TTATCGACGCCATTTTTGTACAGAGCTTTTATCACTTAATCCTGATGCTATTCGGGGGAATGCCTCCGAAATCATGGCAT
TAGCAGGTGTCAGCAATAGTGGGCGCGGTGTTGATAGCACGGATGTGGTAGCGAACGCGTTACCCGCTGCAGTTATGCTG
GCTCAGGAAACTGGTGCAGTGGTAGTGGTTACTGGCGAGGTTGATTATGTTACCGATGGACGACGAACTGTCGGCGTTGG
CGGCGGTGATCCTTTAATGACCAAGGTTGTGGGAACTGGCTGTGCATTATCGGCAGTGGTTGCTGCCTGTTGCGCATTAC
CGGGCGACAGACTGCTAAATGTTGCTTCTGCATGCAGTTGGATGAAGCAAGCAGGAGAACGAGCCATTGTCAGAAGCCAG
GGGCCGGGCACATTTGTGCCGTATTTTCTCGACGCACTCTGGCAGATGACTCAGGAGGTAGAGGCATGA

Upstream 100 bases:

>100_bases
AAGGCTGAGAAATACCCGTATCACCTGATCTGGATAATGCCAGCGTAGGGAAGTCAGACACTGCTCGTGTCCCTTCTTTC
ACGCGGGCAGGAGCTAAAAT

Downstream 100 bases:

>100_bases
AACGGATTAACGCTCTGACAATTGCAGGCACTGATCCCAGTGGTGGTGCAGGAATTCAGGCAGATCTCAAAACGTTCTCT
GCACTTGGTGCTTATGGTTG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQPDLLSRKTAALTLQKFHTLSPLTHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAVASALLINVGTLTQARAH
AMRAAVVHAKRVKTPWTLDPVAVGSLDYRRHFCTELLSLNPDAIRGNASEIMALAGVSNSGRGVDSTDVVANALPAAVML
AQETGAVVVVTGEVDYVTDGRRTVGVGGGDPLMTKVVGTGCALSAVVAACCALPGDRLLNVASACSWMKQAGERAIVRSQ
GPGTFVPYFLDALWQMTQEVEA

Sequences:

>Translated_262_residues
MQPDLLSRKTAALTLQKFHTLSPLTHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAVASALLINVGTLTQARAH
AMRAAVVHAKRVKTPWTLDPVAVGSLDYRRHFCTELLSLNPDAIRGNASEIMALAGVSNSGRGVDSTDVVANALPAAVML
AQETGAVVVVTGEVDYVTDGRRTVGVGGGDPLMTKVVGTGCALSAVVAACCALPGDRLLNVASACSWMKQAGERAIVRSQ
GPGTFVPYFLDALWQMTQEVEA
>Mature_262_residues
MQPDLLSRKTAALTLQKFHTLSPLTHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAVASALLINVGTLTQARAH
AMRAAVVHAKRVKTPWTLDPVAVGSLDYRRHFCTELLSLNPDAIRGNASEIMALAGVSNSGRGVDSTDVVANALPAAVML
AQETGAVVVVTGEVDYVTDGRRTVGVGGGDPLMTKVVGTGCALSAVVAACCALPGDRLLNVASACSWMKQAGERAIVRSQ
GPGTFVPYFLDALWQMTQEVEA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=262, Percent_Identity=79.7709923664122, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6325042, Length=266, Percent_Identity=33.0827067669173, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_ESCF3 (B7LV66)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002383310.1
- EnsemblBacteria:   EBESCT00000125316
- GeneID:   7121991
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_2190
- GeneTree:   EBGT00050000010804
- HOGENOM:   HBG351126
- ProtClustDB:   PRK09355
- BioCyc:   EFER585054:EFER_2190-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27408; Mature: 27408

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 125-125 BINDING 171-171 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDLLSRKTAALTLQKFHTLSPLTHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHH
AAVASALLINVGTLTQARAHAMRAAVVHAKRVKTPWTLDPVAVGSLDYRRHFCTELLSLN
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCC
PDAIRGNASEIMALAGVSNSGRGVDSTDVVANALPAAVMLAQETGAVVVVTGEVDYVTDG
CHHHCCCHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCEEEEEECCEEEEECC
RRTVGVGGGDPLMTKVVGTGCALSAVVAACCALPGDRLLNVASACSWMKQAGERAIVRSQ
CEEEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECC
GPGTFVPYFLDALWQMTQEVEA
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPDLLSRKTAALTLQKFHTLSPLTHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHH
AAVASALLINVGTLTQARAHAMRAAVVHAKRVKTPWTLDPVAVGSLDYRRHFCTELLSLN
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCC
PDAIRGNASEIMALAGVSNSGRGVDSTDVVANALPAAVMLAQETGAVVVVTGEVDYVTDG
CHHHCCCHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCEEEEEECCEEEEECC
RRTVGVGGGDPLMTKVVGTGCALSAVVAACCALPGDRLLNVASACSWMKQAGERAIVRSQ
CEEEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECC
GPGTFVPYFLDALWQMTQEVEA
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA