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The map label for this gene is thiD [H]

Identifier: 218549518

GI number: 218549518

Start: 2234822

End: 2235622

Strand: Reverse

Name: thiD [H]

Synonym: EFER_2189

Alternate gene names: 218549518

Gene position: 2235622-2234822 (Counterclockwise)

Preceding gene: 218549519

Following gene: 218549517

Centisome position: 48.72

GC content: 54.81

Gene sequence:

>801_bases
ATGAAACGGATTAACGCTCTGACAATTGCAGGCACTGATCCCAGTGGTGGTGCAGGAATTCAGGCAGATCTCAAAACGTT
CTCTGCACTTGGTGCTTATGGTTGTTCGGTAATAACCGCCCTGGTAGCGCAAAACACCCGGGGCGTGCAATCGGTGTGTC
GCATAGATCCTGATTTTGTCGCCGCGCAGTTGGATTCGGTGTTCACTGATGTGCGAATCGACACCACTAAAATCGGTATG
TTGGCGGAAACCGATATTGTTGAAGCGGTGGCAGAACGGTTGCAACGTTATCAGATCCAAAACGTGGTACTCGACACCGT
TATGCTGGCAAAAAGCGGCGACCCTCTGCTTTCACCTTCGGCGGTTGCCACGCTGCGCAGTCGATTATTGCCACAGGTTT
CATTAATAACGCCAAACTTGCCCGAAGCAGCCGCATTGCTTGACGCGCCACACGCGCGCAACGAACAGGAAATGCTGGAA
CAAGGACGAGCGCTGTTAGCGATGGGCTGTGGCGCAGTGCTGATGAAAGGCGGTCATCTGGATGATGAGCAAAGCCCGGA
CTGGCTGTTTACCCGAGAGGGGGAACAACGGTTTACTGCACCGCGCATTATGACCAAAAACACTCACGGCACTGGTTGTA
CGCTCTCTGCGGCGTTGGCTGCACTACGCCCGCGCCATACAAACTGGGCTGACACCGTACAGGAGGCAAAAAGTTGGCTT
TCTGCCGCGTTAGCGCAGGCCGACACGCTGGAAGTGGGGCACGGAATTGGTCCGGTTCACCACTTCCACGCCTGGTGGTA
A

Upstream 100 bases:

>100_bases
AGCAAGCAGGAGAACGAGCCATTGTCAGAAGCCAGGGGCCGGGCACATTTGTGCCGTATTTTCTCGACGCACTCTGGCAG
ATGACTCAGGAGGTAGAGGC

Downstream 100 bases:

>100_bases
ACACCGTAATATAGCTGCTTTGGATTTTTTTCAGCCTGCTTGTGCTGAAAATGCAGGGATGAAGCCAATGCAGCTATGGA
TTGCCAGTCGTAAAAGATTT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVCRIDPDFVAAQLDSVFTDVRIDTTKIGM
LAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
SAALAQADTLEVGHGIGPVHHFHAWW

Sequences:

>Translated_266_residues
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVCRIDPDFVAAQLDSVFTDVRIDTTKIGM
LAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
SAALAQADTLEVGHGIGPVHHFHAWW
>Mature_266_residues
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVCRIDPDFVAAQLDSVFTDVRIDTTKIGM
LAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
SAALAQADTLEVGHGIGPVHHFHAWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=97.7443609022556, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=29.6296296296296, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=33.4558823529412, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=34.1911764705882, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28555; Mature: 28555

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVCRIDPDFV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
AAQLDSVFTDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS
HHHHHHHHHHCEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLEQGRALLAMGCGAVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHCCCCC
DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
CCCCCCCEEEECCCCCHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAALAQADTLEVGHGIGPVHHFHAWW
HHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVCRIDPDFV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
AAQLDSVFTDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS
HHHHHHHHHHCEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLEQGRALLAMGCGAVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHCCCCC
DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
CCCCCCCEEEECCCCCHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAALAQADTLEVGHGIGPVHHFHAWW
HHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]