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The map label for this gene is wcaM [H]

Identifier: 218549458

GI number: 218549458

Start: 2155812

End: 2157206

Strand: Reverse

Name: wcaM [H]

Synonym: EFER_2127

Alternate gene names: 218549458

Gene position: 2157206-2155812 (Counterclockwise)

Preceding gene: 218549459

Following gene: 218549457

Centisome position: 47.01

GC content: 46.67

Gene sequence:

>1395_bases
ATGCCATTTAAAAAACTCTCCCGACGCACCTTCCTGACGGCAAGCTCCGCGCTTGCCTTCCTCCATACCCCTTTCGCTCG
CGCACTTCCCGCCCGAACAAGCGTTAACATTAACGACTACAACTCTCACGACTGGATCGCCTCATTTAAACAAGCCTTCA
GCGAAGGGCAAACGGTCGTCGTGCCTGCCGGATTGGTTTGTGACAATATCAACACCGGCATCTTCATCCCCCCTGGTAAA
ACGTTACACATCCTTGGAAGCCTGCGCGGTAACGGCAGAGGGCGTTTTGTCTTACAGGACGGCAGCCAGGTGAAAGGGGA
GAAGGGCGGCAGTATGCATAACATCACCCTGGATGTGCGCGGCTCTGACTGCACCATCAAAGGGCTGGCGATGAGCGGCT
TTGGCCCGGTAACGCAGATTTATATTGGCGGCAAAAACAAACGGGTTATGCGCAACCTGACCATCGATAACCTCACCGTT
AGCCACGCTAACTACGCCATCTTACGCCAGGGATTTCATAACCAGATTATCGGTGCCAATATCACCAATTGTAAGTTCAG
CGACTTACAGGGCGACGCCATTGAATGGAATGTGGCGATTAACGACCGTGATATTTTGATCTCCGACCATCTCATCGAGC
GTATCAACTGTACCAACGGCAAAATCAACTGGGGCATCGGCATAGGCCTTGCGGGGAGTACTTACGATAACAACTACCCG
GAAGACCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGACTGTCGGCAGTTGATTCATGTTGAGAACGG
CAAACATTTCGTCATCCGTAACGTAAATGCGAAGAACATAACACCGGATTACAGCAAAAAAGCAGGTATTGATAACGCAA
CGATTGCCATATATGGCTGTGATAATTTCATAATCGATAATATAGATATAGCTAATAGCGCTGGGTTATTAATTGGTTAT
GGCGTAATCAAAGGAAAATACTTATCGATCCCACAAAATTTTAAGCTCAATAACATTCGTTTGGATAATACACAGCTGAC
CAGAAAATTACGCGGAATTCAGATTTCTGCCGGAAATGCGATCTCTTTTGTTGCACTCACCAATATTGAAATGAAACACG
CATCACTGGAACTACATAACCAGCCGCAACACCTTTTTATGAGAAATATCAATGTGATGCAGGAATTTTCTGTTGGGCCT
GCATTGACCATGAACTTCGACTTGCGCCAGGACGTTCGCGGTAAATTCATGGCTAAAAAAGAGACATTGCTATCTCTGGT
TAATGTTCATGCAGTGAATGAAAAAGGGCAAAATTCAGTCGATATCGACAGGATTAATCACCATGTGGTTAATGTGGAGA
AGGTGAGTTTTGAGCTGCCACCACGCATTAGATAG

Upstream 100 bases:

>100_bases
GTCGTCAAACGTGCTCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCCAGCTTATTGCA
GGCTTTATAGAGGAGAACGT

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCCTGGAAAATTAGAACCATACTTAAGATTATGGCTACTGAAATTTAACTGTGAGCGGCGTAACATCA
GTGGCATATCAGCAAAAGTA

Product: colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARTSVNINDYNSHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVKGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNVNAKNITPDYSKKAGIDNATIAIYGCDNFIIDNIDIANSAGLLIGY
GVIKGKYLSIPQNFKLNNIRLDNTQLTRKLRGIQISAGNAISFVALTNIEMKHASLELHNQPQHLFMRNINVMQEFSVGP
ALTMNFDLRQDVRGKFMAKKETLLSLVNVHAVNEKGQNSVDIDRINHHVVNVEKVSFELPPRIR

Sequences:

>Translated_464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARTSVNINDYNSHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVKGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNVNAKNITPDYSKKAGIDNATIAIYGCDNFIIDNIDIANSAGLLIGY
GVIKGKYLSIPQNFKLNNIRLDNTQLTRKLRGIQISAGNAISFVALTNIEMKHASLELHNQPQHLFMRNINVMQEFSVGP
ALTMNFDLRQDVRGKFMAKKETLLSLVNVHAVNEKGQNSVDIDRINHHVVNVEKVSFELPPRIR
>Mature_463_residues
PFKKLSRRTFLTASSALAFLHTPFARALPARTSVNINDYNSHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVKGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNVNAKNITPDYSKKAGIDNATIAIYGCDNFIIDNIDIANSAGLLIGYG
VIKGKYLSIPQNFKLNNIRLDNTQLTRKLRGIQISAGNAISFVALTNIEMKHASLELHNQPQHLFMRNINVMQEFSVGPA
LTMNFDLRQDVRGKFMAKKETLLSLVNVHAVNEKGQNSVDIDRINHHVVNVEKVSFELPPRIR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=460, Percent_Identity=91.5217391304348, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51228; Mature: 51097

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKKLSRRTFLTASSALAFLHTPFARALPARTSVNINDYNSHDWIASFKQAFSEGQTVV
CCHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVKGEKGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHHCCCCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNVNAKNITPDYSKKAGIDNATIAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCCEEEEEEC
DNFIIDNIDIANSAGLLIGYGVIKGKYLSIPQNFKLNNIRLDNTQLTRKLRGIQISAGNA
CCEEEECEEECCCCCEEEEEEEECCEEECCCCCCEECEEEECCHHHHHHHCCEEEECCCE
ISFVALTNIEMKHASLELHNQPQHLFMRNINVMQEFSVGPALTMNFDLRQDVRGKFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHH
ETLLSLVNVHAVNEKGQNSVDIDRINHHVVNVEKVSFELPPRIR
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKKLSRRTFLTASSALAFLHTPFARALPARTSVNINDYNSHDWIASFKQAFSEGQTVV
CHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVKGEKGGSMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECHHHHHCCCCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNVNAKNITPDYSKKAGIDNATIAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCCEEEEEEC
DNFIIDNIDIANSAGLLIGYGVIKGKYLSIPQNFKLNNIRLDNTQLTRKLRGIQISAGNA
CCEEEECEEECCCCCEEEEEEEECCEEECCCCCCEECEEEECCHHHHHHHCCEEEECCCE
ISFVALTNIEMKHASLELHNQPQHLFMRNINVMQEFSVGPALTMNFDLRQDVRGKFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHH
ETLLSLVNVHAVNEKGQNSVDIDRINHHVVNVEKVSFELPPRIR
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]