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The map label for this gene is dnaB [H]

Identifier: 218549397

GI number: 218549397

Start: 2106745

End: 2108121

Strand: Reverse

Name: dnaB [H]

Synonym: EFER_2067

Alternate gene names: 218549397

Gene position: 2108121-2106745 (Counterclockwise)

Preceding gene: 218549398

Following gene: 218549395

Centisome position: 45.94

GC content: 52.36

Gene sequence:

>1377_bases
ATGAAACAGGATATCGAAGCGAGCGTTATCGGTGGCCTGCTGATTGGTGGATTAACACCAACTGCCAGTGACGTTCTGGC
AACGCTTGAGCCGGAAGCGTTTTCAATTCCGCTCTACCGGAAAGCCTTCGAGGTTATCCGCAAGCAGGCGCGAAACAGAA
ACCTAATCGACGCGCTGATGGTTGCCGAGGCGTGCGGAGAGGAGCATTTCACGTCAATCCTGATGACCAGCAAAAACTGC
CCGAGTGCCGCAAACCTGAAGGGATATGCCGGAATGGTCGCGGATAACTATCACCGCCGTCTGGTGCTGGAAATCATGGA
TGAAATGCGTGAACCAATTCAGAGCGGAACCATCGACGCATCGAGTCAGGCGATGGATGAACTTGTAAAGCGTCTCTCAG
CCATCAGAAAGCCCCGTGACGAGGTTAAACCTGTACGGTTGGGTGAAATCATCACTGACTACACTGACACGCTTGACAGG
CGTCTGAGGAACGGAGAAGAGTCCGATACCCTGAAGACCGGAATCGAAGAACTTGATGCCATCACCGGAGGGATGAACGC
GGAAGACCTGGTGATAATCGCTGCTCGTCCTGGTATGGGTAAAACCGAGCTGGCGCTGAAGATTGCCGAAGGCGTTGCAA
GCCGCGTTATTCCTGGTTCTGACGTCCGGCGAGGAGTGTTGATTTTCTCGATGGAAATGAGCGCATTGCAGATTGCAGAA
CGAAGCATTGCCAACGCCGGGAGGATGTCGGTTAGCGTACTGCGAAATCCTGCATCGATGGATGACGAGGGCTGGGCACG
TGTTGCTAACGGCATGAGTCAGCTTGCGGATTTGGATGTATGGGTAGTCGATGCCTCGCGGTTATCGGTCGAAGAAATAC
GCTCAATCGCAGAACGGCATAAACAGGAAAATCCAAACCTGTCACTCATCATGGCGGATTATCTTGGCCTGATTGAGAAG
CCGAAAGCAGATCGCAACGACCTCGCAATTGCTCACATCTCCGGAAGCCTGAAGGCGATGGCGAAAGACCTGAAAACGCC
TGTTATCTCCCTAAGTCAGCTTTCGCGCGATGTTGAGAAGCGACCAAACAAACGCCCGACAAACGCAGATTTGCGTGATT
CAGGAAGCATTGAACAGGACGCAGACTCAATCATCATGCTCTATCGGGAAGCTGTATATGACGAGAACAGTAGCGCCGCG
CCATTTGCTGAAATCATCGTGACGAAAAACCGTTTTGGCTCGCTTGGTACGGTTTACCAGCAGTTCTGCAACGGACACTT
TGTTGCATGTGACCAGGATGAAGCCAGACAGATTTGCACAACATCAAATGCACCCGGTGCACGTGGCAGACGATATGCAC
AAGGGGCTGACGTATGA

Upstream 100 bases:

>100_bases
GAGACAACGACACTGGCTGGACGGCCGATTTTGATTACCTGCTGAGAGAAGATTCGTTAACGGGAGTTCGTGAAGGGAAG
TTTGCAGACAGGGGGATTGC

Downstream 100 bases:

>100_bases
CCATCTACATCACTGAGCTAATAACAGGGGCTATTTACACAGTAGCCCTTTTTTATTGGATTAAGAACGAGGGGGATCCT
GATGGACACCGTTAACGGAA

Product: Replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQQFCNGHFVACDQDEARQICTTSNAPGARGRRYAQGADV

Sequences:

>Translated_458_residues
MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQQFCNGHFVACDQDEARQICTTSNAPGARGRRYAQGADV
>Mature_458_residues
MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQQFCNGHFVACDQDEARQICTTSNAPGARGRRYAQGADV

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=35.2144469525959, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50111; Mature: 50111

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALM
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
VAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDA
HHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
SSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDRRLRNGEESDTLKTGIEELDA
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHH
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
KQENPNLSLIMADYLGLIEKPKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEK
HCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAAPFAEIIVTKNRFGSLGTVYQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHHHH
QFCNGHFVACDQDEARQICTTSNAPGARGRRYAQGADV
HHHCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALM
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
VAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDA
HHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
SSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDRRLRNGEESDTLKTGIEELDA
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHH
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
KQENPNLSLIMADYLGLIEKPKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEK
HCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAAPFAEIIVTKNRFGSLGTVYQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHHHH
QFCNGHFVACDQDEARQICTTSNAPGARGRRYAQGADV
HHHCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]