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The map label for this gene is ldhA [H]

Identifier: 218548970

GI number: 218548970

Start: 1663595

End: 1664584

Strand: Direct

Name: ldhA [H]

Synonym: EFER_1618

Alternate gene names: 218548970

Gene position: 1663595-1664584 (Clockwise)

Preceding gene: 218548965

Following gene: 218548971

Centisome position: 36.25

GC content: 46.36

Gene sequence:

>990_bases
ATGAAACTCGCTGTATATAGCACGAAACAGTACGATAAAAAGTATCTGCAACAGGTTAATGAGCACTTTGGCTTTGAACT
CGAATTTTTTGATTTTCTGCTGACTGAAAAAACGGCAAAAACTGCCAACGGCTGTGAAGCCGTGTGTATTTTCGTAAATG
ACGATGGCAGTCGTCCGGTACTGGAAGAACTAAAAAAGCACGGCGTGAAATATATCGCTCTGCGTTGTGCTGGCTTTAAT
AACGTTGATCTCGACGCAGCAAAAGAGCTGGGGCTAAAAGTAGTGCGCGTTCCGGCTTATGACCCGGAAGCTGTTGCTGA
ACACGCCATTGGTATGATGATGACGCTGAACCGTCGTATTCATCGTGCGTATCAACGTACTCGTGACGCCAACTTTTCTC
TTGAAGGACTGACTGGTTTTACCATGTACGGTAAAACAGCAGGTGTTATCGGCACCGGGAAAATCGGTGTGGCAATGCTG
CGTATTCTGAAAGGTTTCGGTATGCGCTTGCTGGCATTTGATCCTTATCCAAGTGCTGCTGCTTTGGAGCTGGGTGTGGA
ATATGTCGATCTGCCGACGCTGTTCGCTGAATCGGACGTTATCTCCCTGCACTGCCCGTTAACACCAGAAAACTATCACC
TGCTTAACCATGCAGCGTTTGAGCAGATGAAAGACGGTGTGATGATTATTAATACCAGTCGTGGGGCATTAATCGACTCG
CAGGCTGCGATTGATGCACTGAAAAATCAGAAAATTGGTTCGCTGGGTATGGATGTCTATGAAAACGAAAGGGATCTGTT
CTTTGAAGACAAATCTAATGATGTTATTCAGGATGACGTCTTCCGTCGTTTGTCTGCCTGTCATAACGTGCTGTTTACCG
GGCACCAGGCATTTCTGACCGCAGAAGCATTAACCAGTATTTCTGAAACCACACTGAGCAATCTCAGCCAACTGGTGAAG
GGCGAAACCTGCCCTAATGAACTAGTGTAA

Upstream 100 bases:

>100_bases
TGCGAAGGTTAATAACGCCATAGCTTTCAATTATTTTTTAGATTTTGTAAAATAATTTTAAGACAATGTTTGTGATTTAA
ACATCACTGGAGAAAGCCTT

Downstream 100 bases:

>100_bases
TCTTATCGCTCCCCACCTGTGGGGAGCATTTTCAGATAACCCCTGAAGACAATTTTTACCACATCATTACGATATCGCTT
TTACTGTTCCAGAGAATAAA

Product: fermentative D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLAVYSTKQYDKKYLQQVNEHFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFAESDVISLHCPLTPENYHLLNHAAFEQMKDGVMIINTSRGALIDS
QAAIDALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISETTLSNLSQLVK
GETCPNELV

Sequences:

>Translated_329_residues
MKLAVYSTKQYDKKYLQQVNEHFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFAESDVISLHCPLTPENYHLLNHAAFEQMKDGVMIINTSRGALIDS
QAAIDALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISETTLSNLSQLVK
GETCPNELV
>Mature_329_residues
MKLAVYSTKQYDKKYLQQVNEHFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFAESDVISLHCPLTPENYHLLNHAAFEQMKDGVMIINTSRGALIDS
QAAIDALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISETTLSNLSQLVK
GETCPNELV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=248, Percent_Identity=31.0483870967742, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI61743967, Length=277, Percent_Identity=30.6859205776173, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4557497, Length=277, Percent_Identity=30.6859205776173, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=30.7407407407407, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=30.7407407407407, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI145580575, Length=266, Percent_Identity=31.203007518797, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI6912396, Length=266, Percent_Identity=29.6992481203008, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=96.9604863221885, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=28.2527881040892, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788660, Length=205, Percent_Identity=26.3414634146341, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=28.4172661870504, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=202, Percent_Identity=26.7326732673267, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=193, Percent_Identity=34.7150259067358, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=300, Percent_Identity=29.3333333333333, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=244, Percent_Identity=31.1475409836066, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=31.8518518518519, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=31.8518518518519, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=31.8518518518519, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=31.8518518518519, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=31.8518518518519, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=27.8008298755187, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28574286, Length=260, Percent_Identity=26.1538461538462, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45552429, Length=201, Percent_Identity=29.3532338308458, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585514, Length=201, Percent_Identity=29.3532338308458, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574282, Length=201, Percent_Identity=29.3532338308458, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574284, Length=201, Percent_Identity=29.3532338308458, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45551003, Length=201, Percent_Identity=29.3532338308458, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571528, Length=267, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36522; Mature: 36522

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDKKYLQQVNEHFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFAESDVISLHCPLTPENYHLLNHAAFEQMKDGVMIINTSRGALIDS
HHHHCCEEECCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEECH
QAAIDALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISETTLSNLSQLVKGETCPNELV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLAVYSTKQYDKKYLQQVNEHFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFAESDVISLHCPLTPENYHLLNHAAFEQMKDGVMIINTSRGALIDS
HHHHCCEEECCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEECH
QAAIDALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISETTLSNLSQLVKGETCPNELV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]