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The map label for this gene is lsrK [H]

Identifier: 218548921

GI number: 218548921

Start: 1598197

End: 1599789

Strand: Direct

Name: lsrK [H]

Synonym: EFER_1566

Alternate gene names: 218548921

Gene position: 1598197-1599789 (Clockwise)

Preceding gene: 218548920

Following gene: 218548923

Centisome position: 34.83

GC content: 51.48

Gene sequence:

>1593_bases
ATGGCTCGACTCTTTACCCCTTCAGAATCAAGGCATTACCTGATGGCGCTGGATGCAGGCACCGGAAGTATCCGGGCTGT
GATTTTCGACCTGGAAGGCAATCAAATCGCGGTAGGACAAGCGGAGTGGCGGCATCTGGCAGTACCGGACGTTCCTGGTT
CTATGGAATTTGATCTCAACAAAAACTGGCAACTGGCGTGTGAGTGTATGCGCCAGGCGCTGCATAATTCTGGTATCGCG
CCGGAATACATTGCTGCCGTTTCGGCATGTTCGATGCGTGAAGGTATTGTTTTATATAACAATGAAGGAACACCGATCTG
GGCCTGCGCCAATGTGGATGCCAGAGCGGCGCGGGAGGTCAGTGAACTAAAAGAACTGCATAACAATACCTTTGAAAACG
AAGTGTATCGCGCGACCGGACAAACGCTGGCTTTAAGTGCCATCCCCAGATTACTTTGGCTGGCGCACCATCGTTCCGAT
ATTTACCGTCAGGCATCAACCATCACCATGATAAGCGACTGGCTGGCCTATATGCTCAGCGGTGAACTGGCAGTCGATCC
ATCGAATGCGGGGACTACCGGGCTTCTCGACCTCTCCACCCGTAACTGGAAACCTGCATTGCTGGATATGGCTGGCCTAC
GGGCCGATATTCTTTCTCCTGTCAAAGAAACCGGCACATTGCTGGGCGTGGTAAGTTCACAAGCGGCGGAACTTTGCGGT
CTGAAGGCGGGCACTCCGGTAGTCGTGGGAGGAGGGGATGTACAACTTGGTTGCCTGGGGTTAGGCGTTGTCCGTCCGAC
ACAAACTGCCGTACTTGGCGGTACATTCTGGCAGCAAGTTGTAAACTTAGCCGCACCAGTGACCGATCCGGAAATGAACG
TACGCGTTAATCCCCATGTTATTCCCGGCATGGTACAAGCTGAATCTATTAGCTTTTTTACCGGACTCACCATGCGCTGG
TTCCGCGATGCATTTTGTGCCGAAGAAAAATTGATTGCCGAGCGCTTAGGTATCGATACTTACACGCTGCTTGAAGAGAT
GGCAAGTCGGGTGCCGCCTGGATCGTGGGGCGTGATGCCGATATTCTCTGACAAAATGCGCTTTAAAACCTGGTATCATG
CTGCGCCTTCCTTTATTAATTTGTCTATTGATCCGGATAAATGTAACAAAGCGACATTGTTCCGTGCGCTGGAAGAAAAT
GCGGCGATTGTATCAGCGTGTAACTTGCAGCAAATTGCTGATTTTTCGAATATTCATCCCACATCGTTGGTCTTTGCCGG
CGGAGGTTCTAAAGGGAAATTATGGAGTCAAATTCTCGCTGATGTCTCGGGATTACCCGTCAATATTCCGGTGGTCAAAG
AAGCTACTGCATTAGGATGTGCCATTGCAGCTGGCGTCGGTGCCGGGATTTTTTCATCACTGGCAGAAGCCGGAGAACGC
CTGGTGCGCTGGGAACGGACGCACACACCAGACCCGGAAAAGCATGAACTTTATCAGGATTCACGCGATAAGTGGCAGGC
TGTTTATCAGGATCAGCTGGGGCTGGTTGATCACGGGCTGACAACATCATTATGGAAAGCGCCGGGATTGTAG

Upstream 100 bases:

>100_bases
AGCGGCGATTTTACGTGGTTAAATTTGCATGACCTTATTACCGGGAATGATGGAGAACGTATAGAGGAACAGACAGAACA
CATAAGCCGAGGACAATCTA

Downstream 100 bases:

>100_bases
TGGCATGATGAAAGCCACATTCTGATGGGTGTGGCTGTTAGTTATGCGTTTGGTCAAACTGGACTTTTACCAAAACCATA
TATCAAACCTAAGTTCAGAT

Product: autoinducer-2 (AI-2) kinase

Products: NA

Alternate protein names: AI-2 kinase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MARLFTPSESRHYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNSGIA
PEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLSTRNWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPTQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDKMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSLAEAGER
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Sequences:

>Translated_530_residues
MARLFTPSESRHYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNSGIA
PEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLSTRNWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPTQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDKMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSLAEAGER
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
>Mature_529_residues
ARLFTPSESRHYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNSGIAP
EYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDI
YRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLSTRNWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCGL
KAGTPVVVGGGDVQLGCLGLGVVRPTQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWF
RDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDKMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENA
AIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSLAEAGERL
VRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Specific function: Catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of 4,5-dihydroxy-2,3- pentanedione

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787789, Length=530, Percent_Identity=97.7358490566038, Blast_Score=1075, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=507, Percent_Identity=26.232741617357, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789138, Length=510, Percent_Identity=24.7058823529412, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1790361, Length=513, Percent_Identity=25.5360623781676, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789168, Length=509, Percent_Identity=23.1827111984283, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789987, Length=510, Percent_Identity=25.4901960784314, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17535599, Length=274, Percent_Identity=26.2773722627737, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018484 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: NA

Molecular weight: Translated: 57530; Mature: 57399

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLFTPSESRHYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNSGIAPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLSTRNWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPTQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEEEEECCCCHHEECHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE
IFSDKMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
EECCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHCCCCCC
TSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSLAEAGER
CEEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
ARLFTPSESRHYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNSGIAPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLSTRNWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPTQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEEEEECCCCHHEECHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE
IFSDKMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
EECCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHCCCCCC
TSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSLAEAGER
CEEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA