Click here to switch to the map view.

The map label for this gene is mdtK [H]

Identifier: 218548742

GI number: 218548742

Start: 1417061

End: 1418434

Strand: Reverse

Name: mdtK [H]

Synonym: EFER_1381

Alternate gene names: 218548742

Gene position: 1418434-1417061 (Counterclockwise)

Preceding gene: 218548744

Following gene: 218548741

Centisome position: 30.91

GC content: 50.58

Gene sequence:

>1374_bases
GTGCAGAAGTACATAAATGAAGCGCGTCAGTTATTAGCGTTAGCTATTCCGGTGATTCTCGCGCAAATCGCCCAAACCGC
AATGGGGTTTGTTGATACGGTCATGGCCGGTGGCTACAGTGCCACGGACATGGCGGCAGTTGCTATTGGTACATCTATCT
GGCTACCCGCCATTTTGTTTGGTCATGGCCTGTTACTGGCATTGACTCCGGTTATCGCGCAGCTTAACGGTTCTGGTCGC
CGTGAGCGTATCGCTCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGATTTGTTTCGCTGTTGATTATGCTGGTGCTATG
GAATGCGGGCTATATCATTCGCGCTATGGATAACATTGATCCTGCTCTGGCAGACAAAGCCGTAGGATACTTACGCGCAC
TGCTCTGGGGTGCGCCAGGTTATCTCTTCTTCCAGGTAGCTCGTAACCAGTGTGAGGGACTCGCCAAAACCAAACCGGGA
ATGGTAATGGGATTTATCGGCTTGCTGGTGAACATCCCCGTGAACTATGTGTTCATTTATGGTCATTTCGGTATGCCGGA
GCTGGGTGGTGTTGGTTGTGGCGTTGCGACTGCGGCCGTTTATTGGGTCATGTTTGTCGCTATGCTCTCTTACGTGAAAA
ACGCGCGCTCTATGCGTGATATCCGTAATCAACAGCATTCAGGTAAACCAGATACAGCGGTAATGAAGCGCCTTGTTCAG
TTAGGTTTGCCTATTGCCCTGGCGTTATTCTTTGAGGTTACACTCTTCGCCGTGGTTGCGTTGTTAGTATCCCCACTCGG
AATTGTCGATGTGGCCGGTCACCAGATTGCCCTGAACTTCAGCTCTCTGATGTTCGTATTACCGCTGTCTTTGTCAGCTG
CTGTGACGATCCGCGTTGGTTACCGTCTGGGACAAGGCTCAACACTTGATGCCCAAACAGCTGCTCGTACCGGACTCATG
GTAGGTGTATGTATGGCGACAGTGACGGCGATCTTTACTGTCTCGTTCCGTGAACAAATTGCCCTGCTGTATAACGATAA
TCCGCAAGTCATCACCCTCGCGGCACATTTAATGTTACTGGCTGCGGTTTATCAAATTTCTGACTCAATTCAGGTTATCG
GTAGCGGTATTCTCCGTGGTTATAAAGATACGCGTTCTATCTTCTTTATCACGTTCACCGCTTACTGGGTGTTGGGATTA
CCGAGTGGATATATTCTCGCCCTGACCGACCTGGTCGTGCAACCTATGGGGCCGGCTGGCTTCTGGATTGGCTTTATTAT
TGGTCTGACTTCAGCGGCCATTATGATGATGCTGCGGATGCGTTACTTACAGCGCCTGCCTTCGGCAATTATTCTGCAGC
GTGCTGCGCGATAA

Upstream 100 bases:

>100_bases
TCGCTACAATAGACTGAAAATTCCTCAGATTCTTCTTTTCGCTGCCTCTCGAGGCGGCTTTTTTAGTCTCTCATATAACA
ACAATATAAAAGGTGTTCAC

Downstream 100 bases:

>100_bases
ACCAGCAATAGCCGCCTGACGGCGGCTATTCTGTCAACATTTTGCATGTTTCCTCCGCAATCGAGTGAAAAACGGAAGAA
AATTACCATTTGCCTCTTGC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYINEARQLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSLLIMLVLWNAGYIIRAMDNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYVFIYGHFGMPELGGVGCGVATAAVYWVMFVAMLSYVKNARSMRDIRNQQHSGKPDTAVMKRLVQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPLSLSAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATVTAIFTVSFREQIALLYNDNPQVITLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVQPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRAAR

Sequences:

>Translated_457_residues
MQKYINEARQLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSLLIMLVLWNAGYIIRAMDNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYVFIYGHFGMPELGGVGCGVATAAVYWVMFVAMLSYVKNARSMRDIRNQQHSGKPDTAVMKRLVQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPLSLSAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATVTAIFTVSFREQIALLYNDNPQVITLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVQPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRAAR
>Mature_457_residues
MQKYINEARQLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSLLIMLVLWNAGYIIRAMDNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYVFIYGHFGMPELGGVGCGVATAAVYWVMFVAMLSYVKNARSMRDIRNQQHSGKPDTAVMKRLVQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPLSLSAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATVTAIFTVSFREQIALLYNDNPQVITLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVQPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRAAR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=445, Percent_Identity=21.7977528089888, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI153792564, Length=424, Percent_Identity=21.2264150943396, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI38261960, Length=460, Percent_Identity=20.8695652173913, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=94.0919037199125, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=458, Percent_Identity=23.3624454148472, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321821, Length=388, Percent_Identity=20.360824742268, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320545, Length=439, Percent_Identity=20.2733485193622, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49459; Mature: 49459

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYINEARQLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSLLIMLVLWNAGYIIRAMDNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYVFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFVAMLSYVKNARSMRDIRNQQHSGKPDTAVMKRLVQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPLSLSAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHHHEEEEEEE
YRLGQGSTLDAQTAARTGLMVGVCMATVTAIFTVSFREQIALLYNDNPQVITLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVQPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MQKYINEARQLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSLLIMLVLWNAGYIIRAMDNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYVFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFVAMLSYVKNARSMRDIRNQQHSGKPDTAVMKRLVQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPLSLSAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHHHEEEEEEE
YRLGQGSTLDAQTAARTGLMVGVCMATVTAIFTVSFREQIALLYNDNPQVITLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVQPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRYLQRLPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA