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The map label for this gene is manZ [H]

Identifier: 218548623

GI number: 218548623

Start: 1293781

End: 1294641

Strand: Reverse

Name: manZ [H]

Synonym: EFER_1257

Alternate gene names: 218548623

Gene position: 1294641-1293781 (Counterclockwise)

Preceding gene: 218548624

Following gene: 218548622

Centisome position: 28.21

GC content: 51.57

Gene sequence:

>861_bases
GTGAGCGAAATGGTTGATACAACTCAAAATACCACCGAGAAAAAACTCACTCAGAGTGATATTCGTGGCGTCTTCCTGCG
TTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCGTTGGGCTTCTGCTTCTCTATGGTACCGGCTATTC
GCCGTCTCTACCCTGAGAACAACGATGCCCGTAAACAAGCGATTAAACGTCACCTGGAATTCTTTAACACCCAACCCTTC
GTGGCTGCGCCAATTCTGGGCGTAACCCTGGCACTGGAAGAGCAGCGTGCTAATGGTGCAGAGATCGACGATGGCGCAAT
CAACGGTATCAAAGTTGGTCTGATGGGGCCGCTGGCTGGTGTGGGCGATCCAATCTTCTGGGGTACTGTACGTCCGGTAT
TTGCAGCTCTGGGCGCGGGTATCGCTATGAGCGGAAGCCTGTTAGGGCCGTTGCTGTTCTTTATCCTGTTTAACCTGGTA
CGTCTGGCAACCCGTTACTATGGCGTGGCATATGGTTACAGTAAAGGACTTGATATCGTTAAAGATATGGGCGGTGGCTT
CCTGCAAAAACTGACGGAAGGGGCGTCCATACTCGGCCTGTTTGTCATGGGGGCGCTAGTTAACAAGTGGACGCACGTTA
ATATCCCGATGGTTGTCTCGCGCATCACTGACCAGACGGGTAAAGAACATGTGACGACTGTTCAAACTATTCTTGATCAG
TTAATGCCGGGCCTTGTACCGTTGCTGCTGACTTTCGCCTGTATGTGGCTGCTGCGCAAGAAAGTGAATCCGCTGTGGAT
CATTGTTGGCTTCTTTGTCATTGGTATCGCCGGTTACGCTTGCGGCCTGCTGGGACTGTAA

Upstream 100 bases:

>100_bases
GGTGCTCTACATTCAGCTTAGCCCGAAATATAACCGCGTCGCCGGTGCGCCAGTTCAGGCTGCTGGTAACAACGATCTCG
ACAACGAACTGGACTAACAG

Downstream 100 bases:

>100_bases
TACTGTTGTACACTACCGGGGCCTTTAGGCCCCGTTTTTTTATCTGGAGGATTAATGACAATCACGGAACTGGTACTGAT
TCTCTTCATTGCCGCGTTAC

Product: PTS system mannose-specific transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSEMVDTTQNTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNDARKQAIKRHLEFFNTQPF
VAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLV
RLATRYYGVAYGYSKGLDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPMVVSRITDQTGKEHVTTVQTILDQ
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL

Sequences:

>Translated_286_residues
MSEMVDTTQNTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNDARKQAIKRHLEFFNTQPF
VAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLV
RLATRYYGVAYGYSKGLDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPMVVSRITDQTGKEHVTTVQTILDQ
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
>Mature_285_residues
SEMVDTTQNTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNDARKQAIKRHLEFFNTQPFV
AAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVR
LATRYYGVAYGYSKGLDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPMVVSRITDQTGKEHVTTVQTILDQL
MPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=286, Percent_Identity=98.2517482517483, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789529, Length=275, Percent_Identity=34.5454545454545, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 31292; Mature: 31161

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMVDTTQNTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPEN
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
NDARKQAIKRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHCCCCC
VGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGLDIV
CCCCCHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHH
KDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPMVVSRITDQTGKEHVTTVQTILDQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEMVDTTQNTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPEN
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
NDARKQAIKRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHCCCCC
VGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGLDIV
CCCCCHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHH
KDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPMVVSRITDQTGKEHVTTVQTILDQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]