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The map label for this gene is menE [H]

Identifier: 218548289

GI number: 218548289

Start: 924255

End: 925610

Strand: Direct

Name: menE [H]

Synonym: EFER_0908

Alternate gene names: 218548289

Gene position: 924255-925610 (Clockwise)

Preceding gene: 218548288

Following gene: 218548290

Centisome position: 20.14

GC content: 57.23

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTCAATGATGAGCA
ACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTAGCCTCCGGCTTTGCGGCGCAGGGCGTGGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTCTCCTGGCTGGCGCTACTGCAATGCGGAGCGCGGGTGCTG
CCCGTGAATCCACAGTTGCCGCAACCGTTGCTTGAGGAGTTGTTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
AGGGGAAAACACATTTTCGGCGTTAACGTCGCTGCACATTCAGCTGGTTGAAGGCGCACATGCTGCGGCGTGGCAGCCAA
CTCGTCTGTGCTCAATGACGTTGACCTCGGGTTCTACCGGATTGCCGAAAGCGGCTGTCCATACTTATCAAGCTCATCTT
GCCAGCGCCGAAGGGGTGTTATCGCTGATTCCATTTGGCGAGCACGATGACTGGTTGCTCTCTTTGCCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTACGTGATAAACAGCCGTTGGATCACA
TGCTGGCAGGCTGTACTCACGCATCGCTGGTGCCGACGCAGCTCTGGCGTTTGCTGGTTAACCGCAGTTCCGTTTCCCTG
AAAGCAGTGTTGCTTGGCGGCGCGGCGATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGTATTCGTTGCTTTTG
TGGCTATGGTCTGACCGAGTTTGCTTCCACGGTGTGTGCGAAAGAAGCCGACGGTCTGGCGGACGTCGGTTCGCCGCTGC
CCGGTCGGGAAGTTAAAATCGTTAACGATGAAGTGTGGCTGCGGGCTGCCAGCATGGCAGAAGGTTACTGGCGTAACGGA
CAACTGGTGCCGCTGCTCAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGCGAGATGGATAACGGTAAACTGACCAT
TGTCGGGCGCTTAGATAATCTGTTCTTCAGTGGTGGCGAGGGTATTCAGCCGGAAGAGGTGGAGCGGGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTCCCCGTCGCGGACAAGGAGTTTGGTCATCGACCGGTAGCGGTGGTGGAGTAT
GACCATCAAAGCGTTGATCTCAGCGAATGGGTGAAAGATAAGCTGGCCCGTTTTCAACAACCGGTGCGCTGGCTGACTTT
GCCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAATGGGTGCAACGTCAGCAATAA

Upstream 100 bases:

>100_bases
CGGGGCTGGATACGCTGGATCTGATGCAGGCGCAGCAGGTTCGTCGCTGGCCGGGTAGCACGCTGCCTTTTGTCGACGTT
GATGCACTGGAGCGGTTGTT

Downstream 100 bases:

>100_bases
TTATTGGCTCTCCATTTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATGAAGAGTTTATCTGTTAATCTGTAAATTA
ATATGAAAACTCTTAGCAAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTLLSWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFSALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDHMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QLVPLLNDEGWYATRDRGEMDNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DHQSVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTLLSWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFSALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDHMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QLVPLLNDEGWYATRDRGEMDNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DHQSVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTLLSWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFSALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDHMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QLVPLLNDEGWYATRDRGEMDNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DHQSVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=96.4523281596452, Blast_Score=851, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=350, Percent_Identity=26.8571428571429, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI221142682, Length=492, Percent_Identity=23.5772357723577, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786810, Length=508, Percent_Identity=23.4251968503937, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.9886363636364, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789201, Length=271, Percent_Identity=27.6752767527675, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=461, Percent_Identity=25.5965292841649, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=474, Percent_Identity=25.9493670886076, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=496, Percent_Identity=24.5967741935484, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=500, Percent_Identity=24.4, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=357, Percent_Identity=25.2100840336134, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=399, Percent_Identity=23.5588972431078, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=26.4406779661017, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996755, Length=346, Percent_Identity=23.6994219653179, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=23.0038022813688, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648260, Length=394, Percent_Identity=25.6345177664975, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50097; Mature: 50097

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCEEECCCEEEEEC
NTPQTLLSWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPEGENTFSALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHH
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGEHDDWLL
HEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDHMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQLVPLLNDEGWYATRDRGEMDNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCEEEEEEECCEEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDHQSVDLSEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCEEECCCEEEEEC
NTPQTLLSWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPEGENTFSALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHH
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGEHDDWLL
HEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDHMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQLVPLLNDEGWYATRDRGEMDNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCEEEEEEECCEEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDHQSVDLSEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]